M. A.K. Azad
Laboratory of Forest Products, Department of Forest Science,
Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan
M.N. Amin
Laboratory of Forest Products, Department of Forest Science,
Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan
F. Begum
Laboratory of Forest Products, Department of Forest Science,
Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan
ABSTRACT
The proliferating axillary shoot cultures were established on MS medium supplemented with different concentrations of cytokinins and auxins, using nodal explants from the field grown mature plant of A. vasica. In vitro response of the explants to multiple shoot regeneration varied greatly with the position of the explanting branch on the donor plant. Highest frequencies of shoot formation and maximum number of shoots per explant were obtained on MS medium supplemented with 0.5 mg/l BA. The elongated shoots were rooted successfully on half strength of MS medium with 0.1-0.2 mg/l IBA or without any auxin. The complete plantlets thus regenerated in vitro were successfully transferred to the field.
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How to cite this article
M. A.K. Azad, M.N. Amin and F. Begum, 2003. Rapid Clonal Propagation of a Medicinal Plant - Adhatoda Vasica Nees. Using Tissue Culture Technique. Journal of Biological Sciences, 3: 172-182.
DOI: 10.3923/jbs.2003.172.182
URL: https://scialert.net/abstract/?doi=jbs.2003.172.182
DOI: 10.3923/jbs.2003.172.182
URL: https://scialert.net/abstract/?doi=jbs.2003.172.182
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