Nkechi V. Offiah
School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago
Abiodun A. Adesiyun
Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, South Africa
ABSTRACT
Antimicrobial agents are used for the prevention or treatment of diseases in animals but concerns have been raised that tissues of food animals contaminated with antimicrobial residues may cause adverse side effects in consumers. In Trinidad and Tobago, neither are withdrawal periods following treatment of food animals with antimicrobial agents enforced nor the practice of routine testing of meats for antimicrobial residues. This study determined the frequency of detection of three antimicrobial agents commonly used in the poultry industry in Trinidad and Tobago with the Charm II test and related it to processing plants and sale outlets. For chicken muscles, the prevalence of antimicrobial agents was 18.1, 1.7 and 0.0% for tetracycline, β-lactams and macrolides, respectively and the differences were statistically significantly different (p<0.05; X2) while the corresponding prevalence for chicken liver samples was 52.9, 0.0 and 14.9% (p<0.05; X2). Overall, the frequency of detection of antimicrobial agents in chicken liver (17.8%) was significantly (p<0.05; X2) higher than was found in chicken muscles (6.4%). Although the frequency of detection of the three antimicrobial agents in both chicken muscle and liver tissues was higher in supermarket samples (11.3%) than in those from poultry depot and other sources (6.3%), the difference was not significant (p>0.05; X2). For the 48 tetracycline-positive (muscle and liver) samples, 41 (85.4%) originated from supermarkets while only 14.6% were collected from other sources (p<0.05; X2). The frequency of detection of antimicrobial agents was not significantly (p>0.05; X2) affected by the processing plants from which the samples originated. Tetracycline residues appear to pose the highest health risk (allergic reactions and development of antimicrobial resistance), a finding in agreement with the fact that of the three antimicrobial agents tested, tetracycline was the most commonly used by broiler chicken producers in the country.
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How to cite this article
Nkechi V. Offiah and Abiodun A. Adesiyun, 2015. Detection of Antimicrobial Residues in Chicken Muscle and Liver Sold at Retail Outlets in Trinidad. International Journal of Poultry Science, 14: 456-462.
DOI: 10.3923/ijps.2015.456.462
URL: https://scialert.net/abstract/?doi=ijps.2015.456.462
DOI: 10.3923/ijps.2015.456.462
URL: https://scialert.net/abstract/?doi=ijps.2015.456.462
REFERENCES
- Mumtaz, A., J.A. Awan and M. Athar, 2000. Rational use of drugs in broiler meat production. Int. J. Agric. Biol., 2: 269-272.
Direct Link - Adesiyun, A.A., L.A. Webb and H.T. Romain, 1997. Relatedness of Staphylococcus aureus strains isolated from milk and human handlers in dairy farms in Trinidad. J. Vet. Med. Ser. B, 44: 551-560.
CrossRefDirect Link - Adesiyun, A., N. Offiah, N. Seepersadsingh, S. Rodrigo, V. Lashley, L. Musai and K. Georges, 2005. Microbial health risk posed by table eggs in Trinidad. Epidemiol. Infect., 133: 1049-1056.
CrossRefDirect Link - Agunos, A., D. Leger and C. Carson, 2012. Review of antimicrobial therapy of selected bacterial diseases in broiler chickens in Canada. Can. Vet. J., 53: 1289-1300.
PubMedDirect Link - Al-Ghamdi, M.S., Z.H. Al-Mustafa, F. El-Morsy, A. Al-Faky, I. Haider and H. Essa, 2000. Residues of tetracycline compounds in poultry products in the Eastern Province of Saudi Arabia. Public Health, 114: 300-304.
CrossRefDirect Link - Al-Mazeedi, H.M., A.B. Abbas, H.F. Alomirah, W.Y. Al-Jouhar, S.A. Al-Mufty, M.M. Ezzelregal and R.A. Al-Owaish, 2010. Screening for tetracycline residues in food products of animal origin in the State of Kuwait using charm II radio-immunoassay and LC/MS/MS methods. Food Addit. Contaminants: Part A, 27: 291-301.
CrossRefDirect Link - Berrada, H., F. Borrull, G. Font and R.M. Marce, 2008. Determination of macrolide antibiotics in meat and fish using pressurized liquid extraction and liquid chromatography-mass spectrometry. J. Chromatogr. A, 1208: 83-89.
CrossRefDirect Link - Chauvin, C., S. Le Bouquin-Leneveu, A. Hardy, D. Haguet, J.P. Orand and P. Sanders, 2005. An original system for the continuous monitoring of antimicrobial use in poultry production in France. J. Vet. Pharmacol. Therapeut., 28: 515-523.
CrossRefDirect Link - Chopra, I. and M. Robert, 2001. Tetracycline antibiotics: Mode of action, applications, molecular biology and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev., 65: 232-260.
CrossRefPubMedDirect Link - Darwish, W.S., E.A. Eldaly, M.T. El-Abbasy, Y. Ikenaka, S. Nakayama and M. Ishizuka, 2013. Antibiotic residues in food: The African scenario. Jpn. J. Vet. Res., 61: S13-S22.
CrossRefPubMedDirect Link - De Wasch, K., L. Okerman, H. De Brabander, J. Van Hoof, S. Croubels and P. De Backer, 1998. Detection of residues of tetracycline antibiotics in pork and chicken meat: Correlation between results of screening and confirmatory tests. Analyst, 123: 2737-2741.
CrossRefDirect Link - Dong, C.Y., Y.Y. Liu and Y. Liu, 2012. Determination of sparfloxacin concentrations in chicken serums and residues in chicken tissues and manures using self-ordered ring fluorescence microscopic imaging technique. Spectrosc. Spectral Anal., 32: 2759-2764.
CrossRefDirect Link - Donoghue, D.J., 2003. Antibiotic residues in poultry tissues and eggs: Human health concerns? Poult. Sci., 82: 618-621.
CrossRefPubMedDirect Link - Er, B., F.K. Onurdag, B. Demirhan, S.O. Ozgacar, A.B. Oktem and U. Abbasoglu, 2013. Screening of quinolone antibiotic residues in chicken meat and beef sold in the markets of Ankara, Turkey. Poult. Sci., 92: 2212-2215.
CrossRefDirect Link - Gilchrist, M.J., C. Greko, D.B. Wallinga, G.W. Beran, D.G. Riley and P.S. Thorne, 2007. The potential role of concentrated animal feeding operations in infectious disease epidemics and antibiotic resistance. Environ. Health Perspect., 115: 313-316.
CrossRefPubMedDirect Link - Jiang, W., R.C. Beier, Z. Wang, Y. Wu and J. Shen, 2013. Simultaneous screening analysis of 3-methyl-quinoxaline-2-carboxylic acid and quinoxaline-2-carboxylic acid residues in edible animal tissues by a competitive indirect immunoassay. J. Agric. Food Chem., 61: 10018-10025.
CrossRefDirect Link - Kabir, J., V.J. Umoh, E. Audu-Okoh, J.U. Umoh and J.K.P. Kwaga, 2004. Veterinary drug use in poultry farms and determination of antimicrobial drug residues in commercial eggs and slaughtered chicken in Kaduna State, Nigeria. Food Control, 15: 99-105.
CrossRefDirect Link - Kowalski, P., 2008. Capillary electrophoretic method for the simultaneous determination of tetracycline residues in fish samples. J. Pharmaceut. Biomed. Anal., 47: 487-493.
CrossRefDirect Link - Lolo, M., A.S. Pedreir, J.M. Miranda, B.I. Vazquez, C.M. Franco, A.A. Ceped and C. Fente, 2006. Effect of cooking on enrofloxacin residues in chicken tissue. Food Addit. Contam., 23: 988-993.
CrossRefDirect Link - McCracken, R.J., J.A. van Rhijn and D.G. Kennedy, 2005. The occurrence of nitrofuran metabolites in the tissues of chickens exposed to very low dietary concentrations of the nitrofurans. Food Addit. Contam., 22: 567-572.
CrossRefDirect Link - Macarov, C.A., L. Tong, M. Martinez-Huelamo, M.P. Hermo and E. Chirila et al., 2012. Multi residue determination of the penicillins regulated by the European Union, in bovine, porcine and chicken muscle, by LC-MS/MS. Food Chem., 135: 2612-2621.
CrossRefDirect Link - Mensah, S.E.P., H.Y. Ahissou, O.D. Koudande, S. Salifou, G.A. Mensah and F.A. Abiola, 2011. Detection of antibiotics residues in meat of reformed and marketed laying hens in Southern Benin. Int. J. Biol. Chem. Sci., 5: 2195-2204.
Direct Link - Miranda, J.M., J.A. Rodriguez and C.A. Galan-Vidal, 2009. Simultaneous determination of tetracyclines in poultry muscle by capillary zone electrophoresis. J. Chromatogr. A, 1216: 3366-3371.
CrossRefDirect Link - Olusola, A.V., B.E. Diana and O.I. Ayoade, 2012. Assessment of tetracycline, lead and cadmium residues in frozen chicken vended in Lagos and Ibadan, Nigeria. Pak. J. Biol. Sci., 15: 839-844.
CrossRefPubMedDirect Link - Pavlov, A.I., L. Lashev, I. Vachin and V. Rusev, 2008. Residues of antimicrobial drugs in chicken meat and offals. Trakia J. Sci., 6: 23-25.
Direct Link - Reig, M. and F. Toldra, 2008. Veterinary drug residues in meat: Concerns and rapid methods for detection. Meat Sci., 78: 60-67.
CrossRefDirect Link - Tajick, M.A. and B. Shohreh, 2006. Detection of antibiotics residue in chicken meat using TLC. Int. J. Poult. Sci., 5: 611-612.
CrossRefDirect Link - Weiss, C., A. Conte, C. Milandri, G. Scortichini, P. Semprini, R. Usberti and G. Migliorati, 2007. Veterinary drugs residue monitoring in Italian poultry: Current strategies and possible developments. Food Control., 18: 1068-1076.
CrossRefDirect Link