O. Amarachukwu Igwe
College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria
S. Wilfred Ezema
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
C. Didacus Eze
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
O.A. John Okoye
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
ABSTRACT
Information about the pathogenesis of Newcastle disease (ND) is still limited in many avian species including some poultry birds. Four weeks old cockerels and guinea fowls were inoculated with a local Nigerian velogenic ND (VND) Virus (VNDV) strain, Kudu-113, intramuscularly. The main clinical signs in chickens were severe depression and diarrhoea while the guinea fowls showed mainly leg paralysis. Weight loss was significant in the infected birds in both species from days 3-21 post inoculation (PI). The total mortalities in the guinea fowls and chickens were 22.2 and 94.6%, respectively. The guinea fowls showed no proventricular haemorrhage, intestinal ulcers, haemorrhages and swelling of the caecal tonsil which were all prominent in the cockerels. Gross congestion of the brain was observed in the guinea fowls only. But lesions in the lymphoid organs and microscopic changes in the brain were similar in both species. The antibody response to the viral inoculation was higher in the chickens than the guinea fowls. The above observations confirm experimentally that chickens are more susceptible to VND than guinea fowls. Furthermore, the Kudu-113 infection was viscerotropic in chickens but neurotropic in guinea fowls.
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How to cite this article
O. Amarachukwu Igwe, S. Wilfred Ezema, C. Didacus Eze and O.A. John Okoye, 2014. Experimental Velogenic Newcastle Disease Can Be Very Severe and Viscerotropic in Chickens but Moderate and Neurotropic in Guinea Fowls. International Journal of Poultry Science, 13: 582-590.
DOI: 10.3923/ijps.2014.582.590
URL: https://scialert.net/abstract/?doi=ijps.2014.582.590
DOI: 10.3923/ijps.2014.582.590
URL: https://scialert.net/abstract/?doi=ijps.2014.582.590
REFERENCES
- Brown, C., D.J. King and B.S. Seal, 1999. Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence. Vet. Pathol., 36: 125-132.
CrossRefPubMedDirect Link - Echeonwu, G.O.N., C.U. Iroegbu and A.C. Emeruwa, 1993. Recovery of velogenic newcastle disease virus from dead and healthy free‐roaming birds in Nigeria. Avian Pathol., 22: 383-387.
CrossRefDirect Link - Ezema, W.S., J.O. Okoye and J.A. Nwanta, 2009. LaSota vaccination may not protect against the lesions of velogenic Newcastle disease in chickens. Trop. Anim. Health Prod., 41: 477-484.
CrossRefPubMedDirect Link - Galindo-Muniz, F., N.L. Calderon, M.N. Charles, I.G. Tellez and T.I. Fortoul, 2001. Haematological and histological findings in experimental newcastle disease. Acta Vet. Brno., 70: 185-189.
CrossRefDirect Link - Hamid, H., R.S.F. Campbell and L. Parede, 1991. Studies of the pathology of velogenic Newcastle disease: Virus infection in non-immune and immune birds. Avian Pathol., 20: 561-575.
CrossRefPubMedDirect Link - Haruna, E.S., D. Shamaki, G.O. Echeonwu, K.A. Majiyagbe, Y. Shuaibu and D.R. Du, 1993. A natural outbreak of Newcastle disease in guinea-fowl (Numida meleagris galeata) in Nigeria. Rev. Sci. Technol., 12: 887-893.
Direct Link - Hassan, M.K., M.A. Afify and M.M. Aly, 2004. Genetic resistance of Egyptian chickens to infectious bursal disease and Newcastle disease. Trop. Anim. Health Prod., 36: 1-9.
CrossRefDirect Link - Ibu, J.O., J.O.A. Okoye, D. Eze, F.O. Fasina, E.P. Aba- Adulugba, A.S. Abechi and Y.T. Woma, 2008. Antigenic relatedness among Newcastle disease virus isolates from Nigerian feral birds and the Lasota strain. Arch. Vet. Sci., 13: 79-84.
Direct Link - Ibu, O.J., J.O.A. Okoye, E.P. Adulugba, K.F. Chah and S.V.O. Shoyinka et al., 2009. Prevalence of Newcastle disease viruses in wild and captive birds in Central Nigeria. Int. J. Poult. Sci., 8: 574-578.
CrossRefDirect Link - Jorgensen, P.H., K.J. Handberg, P. Ahrens, H.C. Hansen, R.J. Manvell and D.J. Alexander, 1999. An outbreak of Newcastle disease in free-living pheasants (Phasianus colchicus). Zentralbl. Veterinarmed. B, 46: 381-387.
PubMed - Kapczynski, D.R. and D.J. King, 2005. Protection of chickens against overt clinical disease and determination of viral shedding following vaccination with commercially available Newcastle disease virus vaccines upon challenge with highly virulent virus from the California 2002 exotic Newcastle disease outbreak. Vaccine, 23: 3424-3433.
CrossRefDirect Link - Kawamura, M., K. Nagata-Matsubara, K. Nerome, N. Yamane and H. Kida et al., 1987. Antigenic variation of Newcastle disease viruses isolated from wild ducks in Japan. Microbiol. Immunol., 31: 831-835.
PubMed - Kommers, G.D., D.J. King, B.S. Seal and C.C. Brown, 2001. Virulence of pigeon-origin Newcastle disease virus isolates for domestic chickens. Avian Dis., 45: 906-921.
Direct Link - Kommers, G.D., D.J. King, B.S. Seal and C.C. Brown, 2003. Virulence of six heterogeneous-origin Newcastle disease virus isolates before and after sequential passages in domestic chickens. Avian Pathol., 32: 81-93.
CrossRefDirect Link - Kuiken, T., G. Wobeser, F.A. Leighton, D.M. Haines and B. Chelack et al., 1999. Pathology of Newcastle disease in double-crested cormorants from Saskatchewan, with comparison of diagnostic methods. J. Wildlife Dis., 35: 8-23.
CrossRefDirect Link - Lin, M.Y., H.J. Liu and G.M. Ke, 2003. Genetic and antigenic analysis of Newcastle disease viruses from recent outbreaks in Taiwan. Avian Pathol., 32: 345-350.
CrossRefDirect Link - Mai, H.M., O.D. Ogunsola and O.L. Obasi, 2004. Serological survey of the newcastle disease and infectious bursal disease in local ducks and local guinea fowls in Jos, Plateau State, Nigeria. Rev. Elev. Med. Vet. Pays. Trop., 57: 41-44.
Direct Link - Mathivanan, B., K. Kumanan and A.M. Nainar, 2004. Characterization of a Newcastle disease virus isolated from apparently normal guinea fowl (Numida melagridis). Vet. Res. Commun., 28: 171-177.
Direct Link - Mishra, S., J.M. Kataria, R.L. Sah, K.C. Verma and J.P. Mishra, 2001. Studies on the pathogenicity of Newcastle disease virus isolates in guinea fowl. Trop. Anim. Health Prod., 33: 313-320.
PubMedDirect Link - Nakamura, K., Y. Ohta, Y. Abe, K. Imai and M. Yamada, 2004. Pathogenesis of conjunctivitis caused by Newcastle disease viruses in specific-pathogen-free chickens. Avian Pathol., 33: 371-376.
CrossRefDirect Link - Ojok, L. and C. Brown, 1996. An immunohistochemical study of the pathogenesis of virulent viscerotropic Newcastle disease in chickens. J. Comp. Pathol., 115: 221-227.
PubMed - Okoye, J.O.A., A.O. Agu, C.N. Chineme and G.O.N. Echeonwu, 2000. Pathological characterization in chickens of a velogenic Newcastle disease virus isolated from guinea fowl. Revue d Elevage et de Medicine Veterinaire des Pays Tropicaux, 53: 325-330.
Direct Link - Okoye, J.O.A. and M. Uzoukwu, 1990. Pathogenesis of infectious bursal disease in embryonally bursectomised chickens. Avian Pathol., 19: 555-569.
CrossRefDirect Link - Oladele, S.B., P. Abdu, A.J. Nok, N.D.G. Ibrahim and K.A.N. Esievo, 2008. Pathogenesis of Newcastle disease virus Kudu 113 strain in relation to neuraminidase production in chickens. Vet. Res, 2: 3-8.
Direct Link - Parede, L. and P.L. Young, 1990. The pathogenesis of velogenic newcastle disease virus infection of chickens of different ages and different levels of immunity. Avian Dis., 34: 803-808.
Direct Link - Piacenti, A.M., D.J. King, B.S. Seal, J. Zhang and C.C. Brown, 2006. Pathogenesis of newcastle disease in commercial and specific pathogen-free Turkeys experimentally infected with isolates of different virulence. J. Vet. Pathol., 43: 168-178.
Direct Link - Sa'idu, L., L.B. Tekdek and P.A. Abdu, 2004. Prevalence of Newcastle disease antibodies in domestic and semi-domestic birds in Zaria, Nigeria. Veterinarski Arhiv, 74: 309-317.
Direct Link - Wan, H., L. Chen, L. Wu and X. Liu, 2004. Newcastle disease in geese: Natural occurrence and experimental infection. Avian Pathol., 33: 216-221.
CrossRefDirect Link - Baba, S.S., Iheanacho C.C., J.M. Idris and A.D. El- 2006. Food-based newcastle disease V 4 vaccine in guinea fowl (Numida meleagris galeata pallas) in Trop. Vet., 24: 37-45.
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