S. Sumarsih
Faculty of Animal Agriculture, Diponegoro University, Tembalang Campus, Semarang-50275, Indonesia
B. Sulistiyanto
Faculty of Agriculture Technology, Gadjah Mada University, Jl. Teknika Utara, Bulaksumur, Yogyakarta-55281, Indonesia
C.I. Sutrisno
Faculty of Agriculture Technology, Gadjah Mada University, Jl. Teknika Utara, Bulaksumur, Yogyakarta-55281, Indonesia
E.S. Rahayu
Faculty of Agriculture Technology, Gadjah Mada University, Jl. Teknika Utara,
Bulaksumur, Yogyakarta-55281, Indonesia
ABSTRACT
Characteristics properties of Lactobacillus such as their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt are used in vitro to evaluate their potential as probiotic agent. Objectives of this research are to obtain lactic acid bacteria isolates from Pengging duck intestines and to screen their ability as a probiotic agent. The result showed that the Number of Isolate I11 showed its resistant to low acid (pH 1,5) until 120 min. Some of the strains showed their ability on pH 2,5 during 120 min (No. of isolate I1, I2, I4, I8, I9 and I10). The other isolates decreased their viability to survive at low pH, even they still able to survive at pH 3.5 during 120 min. The result based on the isolates resistance to bile salt showed that isolates could grow at media with 2% of bile salt. Their growth was inhibited with the increasing bile salt concentration. Number of Isolate I11 showed its survival in environment contain 6% bile salt until 120 min. Most isolates had antagonistic against pathogenic bacteria (Salmonella pullorum and Escherichia coli). Based on the characteristics properties. it can be conclude that the 12 isolates of lactic acid bacteria from Pengging duck's caecum were genus Lactobacillus. Isolate number I11 was potentially used as probiotic. Isolate number I11 was identified as Lactobacillus salivarius.
PDF References Citation
How to cite this article
S. Sumarsih, B. Sulistiyanto, C.I. Sutrisno and E.S. Rahayu, 2014. Characteristic of Lactobacillus Isolated from Pengging Duck's Intestines as Probiotics. International Journal of Poultry Science, 13: 47-51.
DOI: 10.3923/ijps.2014.47.51
URL: https://scialert.net/abstract/?doi=ijps.2014.47.51
DOI: 10.3923/ijps.2014.47.51
URL: https://scialert.net/abstract/?doi=ijps.2014.47.51
REFERENCES
- Choi, J.Y., P.L. Shinde, I.K. Kwon, Y.H. Song and B.J. Chae, 2009. Effect of wood vinegar on the performance, nutrient digestibility and intestinal microflora in weanling pigs. Asian-Aust. J. Anim. Sci., 22: 267-274.
Direct Link - Chen, H.L., Y.H. Fan, M.E. Chen and Y. Chan, 2005. Unhydrolyzed and hydrolyzed konjac glucomannans modulated cecal and fecal microflora in Balb/c mice. Nutrition, 21: 1059-1064.
CrossRef - Erdogrul, O. and F. Erbilir, 2006. Isolation and characterization of Lactobacillus bulgaricus and Lactobacillus casei from various foods. Turk. J. Biol., 30: 39-44.
Direct Link - Fuller, R., 2001. The chicken gut microflora and probiotic supplements. J. Poult. Sci., 38: 189-196.
Direct Link - Huang, M.K., Y.J. Choi, R. Houde, J.W. Lee, B. Lee and X. Zhao, 2004. Effects of Lactobacilli and an acidophilic fungus on the production performance and immune responses in broiler chickens. Poult. Sci., 83: 788-795.
CrossRefPubMedDirect Link - Lade, H.S., M.P. Chitanand, G. Gyananath and T. Kadam, 2006. Studies on some properties of bacteriocins produced by Lactobacillus species isolated from agro-based waste. Internet J. Microbiol., Vol. 2, No. 1.
Direct Link - Murry, A.C., A. Hinton and H. Morrision, 2004. Inhibition of growth of Eschericia coli, Salmonella typhimureum, Clostridia perfrigens on chicken feed media by Lactobacillus salivarius and Lactobacillus plantarum. Int. J. Poult. Sci., 3: 603-607.
CrossRefDirect Link - Prasad, J., H. Gill, J. Smart and P.K. Gopal, 1998. Selection and characterisation of Lactobacillus and Bifidobacterium strains for use as probiotics. Int. Dairy J., 8: 993-1002.
CrossRefDirect Link - Saarela, M., G. Mogensen, R. Fonden, J. Matto and T. Mattila-Sandholm, 2000. Probiotic bacteria: Safety, functional and technological properties. J. Biotechnol., 84: 197-215.
CrossRefDirect Link - Shen, W.Y., W.F. Li, Q. Liang, B. Deng, N.N. Chen and X.X. Zhou, 2011. Effects of dietary Bacillus subtilis on growth performance immunity and antioxidant function of grass carp (Ctenopharyngodon idellus). Chin. J. Anim. Nutr., 23: 881-886.
Direct Link - Songisepp, E., J. Kals, T. Kullisaar, R. Mandar, P. Hutt, M. Zilmer and M. Mikelsaar, 2005. Evaluation of the functional efficacy of an antioxidative probiotic in healthy volunteers. Nutr. J., Vol. 4.
CrossRefDirect Link