Sarah Sgavioli
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Vitor Rosa de Almeida
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Maria Fernanda F.M. Praes
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Tamiris Iara Vicentini
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Joao Batista Matos
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Viviane de Souza Morita
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Euclides Braga Malheiros
Department of Exact Sciences, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
Isabel Cristina Boleli
Department of Animal Morphology and Physiology, Faculty of Agricultural and Veterinary Sciences, Sao Paulo State University, UNESP, Brazil
ABSTRACT
This study examined whether preincubation injection of vitamin C intra-eggs influences the eggshell mineral absorption, embryo mortality and hematological characteristics of chicks from eggs incubated at usual or hot temperatures. Five hundred fertile eggs from broiler breeder (Cobb®) were used in an experiment consisting of a 5 x 2 factorial arrangement (five treatments: no injection or injection of 0%, 2, 4, or 6% vitamin C/100 μL water; two incubation temperatures: 37.5°C and 39.0°C). The percentages of vitamin C injected into the eggs did not influence the hematological characteristics of the chicks when compared to non-injected controls. However, chicks from eggs injected with 4% vitamin C had a higher total number of Red Blood Cells (RBCs) and levels of Hematocrit (HCT) and Hemoglobin (HGB) when incubation occurred at hot temperatures. The lowest percentage of Phosphorus (P) from the eggshells incubated at hot temperatures was observed with 2% vitamin C and the highest with 6% vitamin C. Embryo mortality was higher in eggs injected with 2 and 4% of vitamin C than in eggs not injected with vitamin C. The data indicate that intra-eggs injected vitamin C influences the phosphorus absorption from the shell and injection of 4% vitamin C improves potential gas transport in the blood of chicks from eggs incubated at hot temperatures but increases the embryo mortality.
PDF References
How to cite this article
Sarah Sgavioli, Vitor Rosa de Almeida, Maria Fernanda F.M. Praes, Tamiris Iara Vicentini, Joao Batista Matos, Viviane de Souza Morita, Euclides Braga Malheiros and Isabel Cristina Boleli, 2013. Effects of Intra-Egg Injection of Vitamin C on the Eggshell Mineral Absorption,
Embryo Mortality and Hematological Variables in Chicks at Hot Incubation Temperature. International Journal of Poultry Science, 12: 456-463.
DOI: 10.3923/ijps.2013.456.463
URL: https://scialert.net/abstract/?doi=ijps.2013.456.463
DOI: 10.3923/ijps.2013.456.463
URL: https://scialert.net/abstract/?doi=ijps.2013.456.463
REFERENCES
- Belay, T. and R.G. Teeter, 1993. Broiler water balance and thermobalance during thermoneutral and high ambient temperature exposure. Poult. Sci., 72: 116-124.
CrossRefDirect Link - Borges, S.A., A. Maiorka, A.C. Laurentiz, A.V. Fischer da Silva, E. Santini and J. Ariki, 2002. Balanco eletrolitico para frangos de corte na primeira semana de idade [Electrolyte balance for broiler in first week of age]. Rev. Brasileira de Ciencia Avicola, 4: 149-153.
Direct Link - Borges, S.A., A.V.F. da Silva, J. Ariki, D.M. Hooge and K.R. Cummings, 2003. Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat-stress environments. Poult. Sci., 82: 428-435.
Direct Link - Finkler, M.S., J.B. van Orman and P.R. Sotherland, 1998. Experimental manipulation of egg quality in chickens: Influence of albumen and yolk on the size and body composition of near-term embryos in a precocial bird. J. Comp. Physiol., 168(B): 17-24.
PubMedDirect Link - French, N.A., 1997. Modeling incubation temperature: The effects of incubator design, embryonic development and egg size. Poult. Sci., 76: 124-133.
CrossRefDirect Link - Ghonim, A.I.A., A.L. Awad, M.H.A. Fattouh and A.M. El-Shhat, 2009. Comparative study of ascorbic acid treatment methods on hatchability traits and growth performance of ducklings. Egypt. Poult. Sci. J., 29: 1085-1099.
Direct Link - Mahmoud, K.Z., F.W. Edens, E.J. Eisen and G.B. Havenstein, 2004. Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers (Gallus gallus domesticus) subjected to cyclic heat stress. Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 137: 35-42.
CrossRefDirect Link - Mohammed, K.A., A. El-Boghdady, M.A.H. Soliman, M.A. Abd Al-Galil and N.M. Abd Al-Aleem, 2011. The effect of both pre-incubation dipping eggs in vitamin C and cooling eggs during incubation period on embryonic and hatchability parameters in two local chicken strains. Egypt. Poult. Sci., 31: 379-392.
Direct Link - Molenaar, R., I. van den Anker, R. Meijerhof, B. Kemp and H. van den Brand, 2011. Effect of eggshell temperature and oxygen concentration during incubation on the developmental and physiological status of broiler hatchlings in the perinatal period. Poult. Sci., 90: 1257-1266.
CrossRefPubMedDirect Link - Mushtaq, T., M. Sarwar, H. Nawaz, M.A. Mirza and T. Ahmad, 2005. Effect and interactions of dietary sodium and chloride on broiler starter performance (hatching to twenty-eight days of age) under subtropical summer conditions. Poult. Sci., 84: 1716-1722.
CrossRefPubMedDirect Link - Nowaczewski, S., H. Kontecka and S. Krystianiak, 2012. Effect of in ovo injection of vitamin C during incubation on hatchability of chickens and ducks. Folia Biologica, 60: 93-97.
CrossRefDirect Link - Pardue, S.L. and J.P. Thaxton, 1986. Ascorbic acid in poultry: A review. World's Poult. Sci. J., 42: 107-123.
CrossRefDirect Link - Rahn, H., V.L. Christensen and F.W. Edens, 1981. Changes in shell conductance, pores and physical dimensions of egg and shell during the first breeding cycle of turkey hens. Poult. Sci., 60: 2536-2541.
CrossRefDirect Link - Richards, S.A., 1971. The significance of changes in the temperature of the skin and body core of the chicken in the regulation of heat loss. J. Physiol., 216: 1-10.
CrossRefPubMedDirect Link - Robbins, K.R., H.W. Norton and D.H. Baker, 1979. Estimation of nutrient requirements from growth data. J. Nutr., 109: 1710-1714.
Direct Link - Tzschentke, B. and A. Plagemann, 2006. Imprinting and critical periods in early development. World's Poult. Sci. J., 62: 626-637.
CrossRef - Tzschentke, B., 2007. Attainment of thermoregulation as affected by environmental factors. Poult. Sci., 86: 1025-1036.
CrossRefDirect Link - Zakaria, A.H. and M.A. Al-Anezi, 1996. Effect of ascorbic acid and cooling during egg incubation on hatchability, culling, mortality and the body weights of broiler chickens. Poult. Sci., 75: 1204-1209.
CrossRefPubMedDirect Link