M.H. Tamzil
Faculty of Animal Sciences, Mataram University, Jl. Majapahit Mataram, Lombok, West Nusa Tenggara-83125, Indonesia
R.R. Noor
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
P.S. Hardjosworo
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
W. Manalu
Faculty of Veterinary Medicine, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
C. Sumantri
Faculty of Animal Sciences, Bogor Agricultural University, Jl. Agatis IPB Campus, Darmaga, Bogor, West Java-16680, Indonesia
ABSTRACT
An experiment was conducted to study the response of three chicken lines with different HSP 70 genotypes to acute heat stress. Twenty eight kampong chicken (native chicken, with seven genotypes i.e., AA, AB, AC, CC, AD, DD and BC) and twenty four Arabic chicken (with six genotypes i.e., AA, AB, AC, CC, AD and BC) and four commercial chickens (with one genotype i.e., DD) were used and randomly allocated in a factorial arrangement into groups which their HSP 70 genotypes had been identified. Acute heat stress was exposed at 40°C for 0.5, 1.0 and 1.5 h, respectively, using chamber in 33 x 33 x 75 cm3. Parameters measured were the onset of panting (minute), panting frequency (times/minute), feed consumption (g/minute), water consumption (mL/minute), manure water content (%), rectal temperature (°C), serum corticosterone concentration (μg/dL) and HSP 70 expression (copy mRNA). The result of this study showed that there was an interaction between chicken lines and acute heat stress exposure on water consumption and manure water content. Chicken lines affected panting frequencies and manure water content but it did not affect the onset of panting, feed and water consumptions, rectal temperature, serum corticosterone concentration and expression of HSP 70. Acute heat stress increased panting frequency, drinking water consumption, manure water content, rectal temperature, serum corticosterone concentration, HSP 70 expression and it decreased feed consumption. The highest response on panting frequency, rectal temperature, serum corticosterone concentration and expression of HSP 70 was found in the DD genotype and the lowest in AD genotype. The most rapid onset of panting occurred in DD genotype and the slowest in AD genotype. The study revealed interaction between chicken lines and HSP 70 genotypes in heat resistance. Kampong chicken had the highest heat resistance as compared to Arabic and commercial chickens but HSP 70 genotypes that was the most tolerant to high ambient temperature was AD genotype where as the lowest tolerant was DD genotype.
PDF References Citation
How to cite this article
M.H. Tamzil, R.R. Noor, P.S. Hardjosworo, W. Manalu and C. Sumantri, 2013. Acute Heat Stress Responses of Three Lines of Chickens with Different Heat Shock Protein (HSP)-70 Genotypes. International Journal of Poultry Science, 12: 264-272.
DOI: 10.3923/ijps.2013.264.272
URL: https://scialert.net/abstract/?doi=ijps.2013.264.272
DOI: 10.3923/ijps.2013.264.272
URL: https://scialert.net/abstract/?doi=ijps.2013.264.272
REFERENCES
- Ajakaiye, J.J., J.O. Ayo and S.A. Ojo, 2010. Effects of heat stress on some blood parameters and egg production of Shika Brown layer chickens transported by road. Biol. Res., 43: 183-189.
CrossRefDirect Link - Al-Aqil, A. and I. Zulkifli, 2009. Changes in heat shock protein 70 expression and blood characteristics in transported broiler chickens as affected by housing and early age feed restriction. Poult. Sci., 88: 1358-1364.
CrossRefDirect Link - Al-Fataftah, A.R.A. and Z.H.M. Abu-Dieyeh, 2007. Effect of chronic heat stress on broiler performance in Jordan. Int. J. Poult. Sci., 6: 64-70.
CrossRefDirect Link - Zahraa and H. Al-Ghamdi, 2008. Effects of commutative heat stress on immunoresponses in broiler chickens reared in closed system. Int. J. Poult. Sci., 7: 964-968.
CrossRefDirect Link - Altan, O., A. Altan, M. Cabuk and H. Bayraktar, 2000. Effects of heat stress on some blood parameters in broilers. Turk. J. Vet. Anim. Sci., 24: 145-148.
Direct Link - Collier, R.J., J.L. Collier, R.P. Rhoads and L.H. Baumgard, 2008. Invited review: Genes involved in the bovine heat stress response. J. Dairy Sci., 91: 445-454.
CrossRefDirect Link - Cooper, M.A. and K.W. Washburn, 1998. The relationships of body temperature to weight gain, feed consumption and feed utilization in broilers under heat stress. Poult. Sci., 77: 237-242.
CrossRefPubMedDirect Link - Fumihito, A., T. Miyake, M. Takada, R. Shingu and M.T. Endo et al., 1996. Monophyletic origin and unique dispersal patterns of domestic fowls. Proc. Natl. Acad. Sci. USA., 93: 6792-6795.
Direct Link - Gabriel, J.E., J.A. Ferro, R.M.P. Stefani, M.I.T. Ferro, S.L. Gomes and M. Macari, 1996. Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers. Br. Poult. Sci., 37: 443-449.
CrossRefPubMedDirect Link - Garriga, C., R.R. Hunter, C. Amat, J.M. Planas, M.A. Mitchell and M. Moreto, 2006. Heat stress increases apical glucose transport in the chicken jejunum. Am. J. Physio. Regul. Integer. Comp. Physiol., 290: 195-201.
PubMedDirect Link - Gaviol, H.C.T., E. Gasparino, A.J. Prioli and M.A. Soares, 2008. Genetic evaluation of the HSP70 protein in the Japanese quail (Coturnix japonica). Genet. Mol. Res., 7: 133-139.
PubMed - Lin, H., H.F. Zhang, R. Du, X.H. Gu, Z.Y. Zhang, J. Buyse and E. Decuypere, 2005. Thermoregulation responses of broiler chickens to humidity at different ambient temperatures. II. Four weeks of age. Poult. Sci., 84: 1173-1178.
CrossRefPubMedDirect Link - Mazzi, C.M., J.A. Ferro, M.I.T. Ferro, V.J.M. Savino, A.A.D. Coelho and M. Macari, 2003. Polymorphism analysis of the hsp70 stress gene in Broiler chickens (Gallus gallus) of different breeds. Genet. Mol. Biol., 26: 275-281.
CrossRefDirect Link - Mahmoud, K.Z., F.W. Eden, E.J. Eisen and G.B. Havenstein, 2004. The effect of dietary phosphorus on heat shock protein mRNAs during acute heat stress in male broiler chickens (Gallus gallus). Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol., 137: 11-18.
CrossRef - Nishida, T., Y. Hayashi, K. Nozawa, T. Hashiguchi and S.S. Mansjoer, 1988. Morphological studies on the Indonesian native chicken. Jap. J. Zootechn. Sci., 59: 1047-1058.
CrossRefDirect Link - Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chickens 1. Response parameters. Poult. Sci., 79: 363-369.
CrossRefDirect Link - Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chickens 3. Temporal patterns of response. Poult. Sci., 79: 377-382.
CrossRefPubMedDirect Link - Sohail, M.U., A. Ijaz, M.S. Yousaf, K. Ashraf, H. Zaneb, M. Aleem and H. Rehman, 2010. Alleviation of cyclic heat stress in broilers by dietary supplementation of mannan-oligosaccharide and Lactobacillus-based probiotic: Dynamics of cortisol, thyroid hormones, cholesterol, C-reactive protein and humoral immunity. Poult. Sci., 89: 1934-1938.
CrossRefPubMedDirect Link - Spasojevi, N., L. Gavrilovi, I. Kovacevi and S. Dronjak, 2007. Endocrinological and behavioural effects of chronic fluxilan administration in rats. JMB, 26: 274-279.
CrossRef - Sullivan, D.A. and C.R. Wira, 1979. Sex hormone and glucocorticoid receptors in the bursa of Fabricius of immature chicks. J. Immunol., 122: 2617-2623.
Direct Link - Vahdatpour, T., K.N. Adl, Y.E. Nezhad, N.M. Sis, S.R. Riyazi and S. Vahdatpour, 2009. Effects of corticosterone intake as stress-alternative hormone on broiler chickens: Performance and blood parameters. Asian J. Anim. Vet. Adv., 4: 16-21.
CrossRefDirect Link - Gabriel, J.E., J.A. Ferro, R.M.P. Stefani, M.I.T. Ferro, S.L. Gomes and M. Macari, 1996. Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers. Br. Poult. Sci., 37: 443-449.
CrossRefPubMedDirect Link - Yu, J. and E. Bao, 2008. Effect of acute heat stress on heat shock protein 70 and its corresponding mRNA expression in the heart, liver and kidney of broilers. Asian-Aust. J. Anim. Sci., 21: 1116-1126.
CrossRefDirect Link - Zhen, F.S., H.L. Du, H.P. Xu, Q.B. Luo and X.Q. Zhang, 2006. Tissue and allelic-specific expression of HSP 70 gene in chickens: Basal and heat-stress-induced mRNA level quantified with real-time reverse transcriptase polymerase chain reaction. Br. Poul. Sci., 47: 449-455.
CrossRef - Zulkifli, I., A. Al-Aqil, A.R. Omar, A.Q. Sazili and M.A. Rajion, 2009. Crating and heat stress influence blood parameters and heat shock protein 70 expression in broiler chickens showing short or long tonic immobility reactions. Poult. Sci., 88: 471-476.
CrossRefDirect Link