B.R. Landers
Division of Animal Science, University of Missouri, Columbia 65211, USA
R.A. Murarolli
Division of Animal Science, University of Missouri, Columbia 65211, USA
R.E. Gelven
Division of Animal Science, University of Missouri, Columbia 65211, USA
L.M. Brand
Division of Animal Science, University of Missouri, Columbia 65211, USA
E.H. Kobashigawa
Division of Animal Science, University of Missouri, Columbia 65211, USA
D.R. Ledoux
Division of Animal Science, University of Missouri, Columbia 65211, USA
A.J. Bermudez
Department of Veterinary Pathobiology, University of Missouri, Columbia 65211, USA
G.E. Rottinghaus
Department of Biomedical Science, University of Missouri, Columbia 65211, USA
ABSTRACT
An experiment was conducted with Pekin ducks to determine the toxicity of melamine (MEL) and cyanuric acid (CYA) in ducks fed treatments from day 3 to day 21 of age. Two hundred and twenty three three-day-old male ducks were assigned to one of 10 treatment groups. Treatments included: (1) a basal diet (BD) containing no MEL or CYA; (2) BD+0.5% MEL; (3) BD+1.0% MEL; (4) BD+1.5% MEL; (5) BD+0.5% CYA; (6) BD+1.0% CYA; (7) BD+1.5% CYA; (8) BD+0.5% MEL+0.5% CYA; (9) BD+1.0% MEL+1.0% CYA and (10) BD+1.5% MEL+1.5% CYA. Control and treatments fed MEL alone or MEL+CYA were fed to 5 pens of 5 ducks each. Treatments fed CYA alone were fed to 4 pens of 4 ducks each. Compared to controls, birds fed > 1.0% MEL had lower (P<0.05) feed intake (FI) and body weight gain (BWG), heavier (P<0.05) relative kidney weights and higher (P<0.05) mortality. No mortality was observed in birds fed CYA alone or MEL+CYA combinations. No differences in FI, BWG, relative kidney weights or mortality were noted among controls and ducks fed CYA alone or MEL+CYA combinations. Melamine crystals were only observed in the bile of ducks fed>1.0% MEL alone. Renal histopathology included mild dilation of the embryonal nephrons and collecting tubules. Eosinophilic to basophilic casts, some containing spherical eosinophilic crystals were also present in the embryonal nephrons and collecting tubules. Histopathology results suggested that >1.00 % MEL in the diet of ducks could cause severe renal pathology and mortality due to renal failure. The renal pathology observed in ducks was similar to that seen in other poultry species fed toxic concentrations of MEL. CYA alone up to 1.5% of the diet was not toxic to ducks and CYA reduced the toxicity of MEL when the two compounds were fed in combination.
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How to cite this article
B.R. Landers, R.A. Murarolli, R.E. Gelven, L.M. Brand, E.H. Kobashigawa, D.R. Ledoux, A.J. Bermudez and G.E. Rottinghaus, 2013. Individual and Combined Effects of Melamine and Cyanuric Acid in Young Pekin
Ducks. International Journal of Poultry Science, 12: 192-201.
DOI: 10.3923/ijps.2013.192.201
URL: https://scialert.net/abstract/?doi=ijps.2013.192.201
DOI: 10.3923/ijps.2013.192.201
URL: https://scialert.net/abstract/?doi=ijps.2013.192.201
REFERENCES
- Lv, X., J. Wang, L. Wu, J. Qiu, J. Li, Z. Wu and Y. Qin, 2010. Tissue deposition and residue depletion in lambs exposed to melamine and cyanuric acid-contaminated diets. J. Agric. Food Chem., 58: 943-948.
CrossRefPubMedDirect Link - Baynes, R.E. and J.E. Riviere, 2010. Risks associated with melamine and related triazine contamination of food. Emerging Health Threats J., Vol. 3.
CrossRefDirect Link - Brand, L.M., R.A. Murarolli, R.E. Gelven, D.R. Ledoux and B.R. Landers et al., 2012. Effects of melamine in young broiler chicks. Poult. Sci., 91: 2022-2029.
CrossRefDirect Link - Brown, C.A., K.S. Jeong, R.H. Poppenga, B. Puschner and D.M. Miller et al., 2007. Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007. J. Vet. Diagn. Invest., 19: 525-531.
CrossRefPubMedDirect Link - Buur, L.J., E.R. Baynes and E.J. Riviere, 2008. Estimating meat withdrawal times in pigs exposed to melamine contaminated feed using a physiologically based pharmacokinetic model. Regul. Toxicol. Pharm., 51: 324-331.
CrossRefPubMedDirect Link - Conover, W.J. and R.L. Iman, 1981. Rank transformations as a bridge between parametric and nonparametric statistics. Am. Statistician, 35: 124-129.
CrossRefDirect Link - Ding, X.M., S.P. Bai, K.Y. Zhang, L. Wang and C.M. Wu et al., 2012. Tissue deposition and residue depletion in broiler exposed to melamine-contaminated diets. J. Integrat. Agric., 11: 109-115.
CrossRefDirect Link - Dobson, R.L.M., S. Motlagh, M. Quijano, R.T. Cambron and T.R. Baker et al., 2008. Identification and characterization of toxicity of contaminants in pet food leading to an outbreak of renal toxicity in cats and dogs. Toxicol. Sci., 106: 251-262.
CrossRefPubMedDirect Link - Filigenzi, M.S., E.R. Tor, R.H. Poppenga, L.A. Aston and B. Puschner, 2007. The determination of melamine in muscle tissue by liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 21: 4027-4032.
PubMed - Gao, C.Q., S.G. Wu, H.Y. Yue, F. Ji and H.J. Zhang et al., 2010. Toxicity of dietary melamine to laying ducks: Biochemical and histopathological changes and residue in eggs. J. Agric. Food Chem., 58: 5199-5208.
CrossRefDirect Link - Jutzi, K., A.M. Cook and R. Hutter, 1982. The degradative pathway of s-triazine melamine: The steps to ring cleavage. Biochem. J., 208: 679-684.
PubMed - Karbiwnyk, C.M., W.C. Andersen, S.B. Turnipseed, J.M. Storey and M.R. Madson et al., 2009. Determination of cyanuric acid residues in catfish, trout, tilapia, salmon and shrimp by liquid chromatography-tandem mass spectrometry. Anal. Chim. Acta, 637: 101-111.
CrossRefPubMedDirect Link - Brand, L.M., R.A. Murarolli, R.E. Gelven, D.R. Ledoux and B.R. Landers et al., 2012. Effects of melamine in young broiler chicks. Poult. Sci., 91: 2022-2029.
CrossRefDirect Link - Lipschitz, W.L. and E. Stockey, 1945. The mode of action of three new diuretics: Melamine, adenine and formoguanamine. J. Pharmacol. Exp. Therapeutics., 83: 235-249.
Direct Link - Lu, M.B., L. Yan, J.Y. Guo, Y. Li, G.P. Li and V. Ravindran, 2009. Melamine residues in tissues of broilers fed diets containing graded levels of melamine. Poult. Sci., 88: 2167-2170.
CrossRefDirect Link - NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Direct Link - Perdigao, L.M.A., N.R. Champness and P.H. Beton, 2006. Surface self-assembly of the cyanuric acid-melamine hydrogen bonded network. Chem. Commun.
CrossRefDirect Link - Reimschuessel, R., C.M. Gieseker, R.A. Miller, J. Ward and J. Boehmer et al., 2008. Evaluation of the renal effects of experimental feeding of melamine and cyanuric acid to fish and pigs. Am. J. Vet. Res., 69: 1217-1228.
CrossRef - Stine, C.B., R. Reimschuessel, C.M. Gieseker, E.R. Evans and T.D. Mayer et al., 2011. A No Observable Adverse Effects Level (NOAEL) for pigs fed melamine and cyanuric acid. Regul. Toxicol. Pharmacol., 60: 363-372.
CrossRef - Tyan, Y.C., M.H. Yang, S.B. Jong, C.K. Wang and J. Shiea, 2009. Melamine contamination. Anal. Bioanal. Chem., 395: 729-735.
CrossRefDirect Link - Wackett, L.P., M.J. Sadowsky, B. Martinez and N. Shapir, 2002. Biodegradation of atrazine and related s-triazine compounds: From enzymes to field studies. Applied Microbiol. Biotech., 58: 39-45.
PubMed - Yan, L., J. Guo, Z. Sun, S. Zhu, J. Rong, L. Sui and M. Lu, 2009. Melamine residues in tissues of ducks fed diets containing graded levels of melamine. China Poult., 13: 16-19.
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