S. Murtini
Department of Animal Diseases and Veterinary Public Health, Bogor Agriculture University, Bogor, Indonesia
R. Murwani
Laboratory of Nutritional Biochemistry, Department of Nutrition and Feed Science, Faculty of Animal Agriculture, Diponegoro University, Semarang, Indonesia
F. Satrija
Department of Animal Diseases and Veterinary Public Health, Bogor Agriculture University, Bogor, Indonesia
E. Handaryani
Department of Clinical Reproduction and Pathology, Bogor Agriculture University, Bogor, Indonesia
ABSTRACT
S. oortiana extract has been previously shown to have anti-tumour and antiviral activity in vitro. This study was carried out to investigate an antiviral effect of S. oortiana stem extract against Mareks Disease Virus (MDV) in ovo. The antiviral effect was studied in embryonated chicken eggs (n = 5 per treatment) by inoculating 0, 0.1, 0.2, or 0.4 mg extract/egg before or after infection with MDV. In the before treatment, the extract was administered on day 9 with MDV infection on day 12. In the after treatment, the extract was administered on day 12 after infection with MDV on day 9. Histopathological examinations were performed on day 20 to determine the number of inclusion bodies, pock formation, macrophage infiltration and immunohistochemistry was used to determine the frequency and intensity of viral antigen expression. Inoculation of the extract before MDV infection significantly (p<0.05) reduced the frequency and intensity of viral antigen expression. There was also a significantly lower level of epithelial destruction on chorioallantoic membrane of the groups given 0.2 and 0.4 mg extract/egg than the control group given no extract. This study provides the first evidence of anti MDV viral activity of S. oortiana extract in vivo and may provide an alternative method to prevent or alleviate Mareks disease.
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How to cite this article
S. Murtini, R. Murwani, F. Satrija and E. Handaryani, 2010. Anti Mareks Disease Virus Activity of Scurrula oortiana (Tea Mistletoe) Stem Extract in Embryonated Chicken Eggs*. International Journal of Poultry Science, 9: 879-885.
DOI: 10.3923/ijps.2010.879.885
URL: https://scialert.net/abstract/?doi=ijps.2010.879.885
DOI: 10.3923/ijps.2010.879.885
URL: https://scialert.net/abstract/?doi=ijps.2010.879.885
REFERENCES
- Calabrese, E.J. and L.A. Baldwin, 1999. Reevaluation of the fundamental dose-response relationship. Bioscience, 49: 725-734.
Direct Link - Cho, K.O., K. Ohashi and M. Onuma, 1999. Electron microscopic and immunohistochemical localization of Marek's Disease (MD) herpesvirus particles in MD skin lymphomas. Vet. Pathol., 36: 314-320.
CrossRef - Kusumoto, I.T., I. Shimada, N. Kakiuchi, M. Hattori, T. Namba and S. Supriyatna, 1992. Inhibitory effects of Indonesian plant extracts on reverse transcriptase of an RNA tumour virus (I). Phytother. Res., 6: 241-244.
CrossRefDirect Link - Murwani, R., 2003. Indonesian tea mistletoe (Scurrula oortiana) stem extract increases tumour cell sensitivity to tumour necrosis factor alpha (TNF-α). Phytother. Res., 17: 407-409.
CrossRef - Ohashi, K., H. Winarno, M. Mukai, M. Inoue, M.S. Prana, P. Simanjuntak and H. Shibuya, 2003. Indonesian medicinal plants XXV cancer cell invasion inhibitory effects of chemical contituents in the parasitic plant Scurrula atropurpurea (Loranthaceae). Chem. Pharm. Bull., 51: 343-345.
Direct Link - Rosner, K., C. Ropke, V. Pless and G.L. Skovgaard, 2006. Late type apoptosis and apoptosis free lethal effect of quercetin in human melanoma. J. Biosci. Biotechnol. Biochem., 70: 2169-2177.
Direct Link - Schat, K.A., 2004. Understanding marek's disease immunity: A continuing challenge. Int. J. Poult. Sci., 3: 89-95.
CrossRefDirect Link - Veckenstedt, A. and R. Pusztai, 1981. Mechanism of antiviral action of quercetin against cardiovirus infection in mice. J. Antiviral Res., 4: 249-261.
CrossRef - Witter, R.L., 2001. Marek's Disease Vaccines-Past, Present and Future (Chicken vs virus-a Battle of the Centuries). In: Current Progress on Marek's Disease Research, Schat, K.A., R.W. Morgan, M.S. Parcells and J.L. Spencer (Eds.). American Association of Avian Pathologists, Kennett Square, Pennsylvania.
- Xing, Z. and K.A. Schat, 2000. Inhibitory effects of nitric oxide and gamma interferon on in vitro and in vivo replication of Marek's disease virus. J. Virol., 74: 3605-3612.
Direct Link - Xing, Z. and K.A. Schat, 2000. Expression of cytokine genes in Marek's disease virus-infected chickens and chicken embryo fibroblast cultures. Immunology, 100: 70-76.
CrossRef