P. Ponnusamy
Department of Veterinary Microbiology, Madras Veterinary College, Chennai-600 007, India
J. John Kirubaharan
Department of Veterinary Microbiology, Madras Veterinary College, Chennai-600 007, India
Andrew Chandramohan
Department of Veterinary Microbiology, Madras Veterinary College, Chennai-600 007, India
ABSTRACT
The HN and F genes of the D58 isolate have coding senquence of 577 and 553 amino acids respectively. The HN gene has stalk (1-143) and globular head (123-571) regions with transmembrane domain at positions 25-45, sialic acid residue at 234-239, glycosylation sites at 119, 341, 433, 481 and 538 and cysteine residues at 172, 186, 196, 238, 247, 251, 344, 455, 461, 465, 531 and 542. The glycosylation site at 508 specific for virulent strains and cysteine residue at 123 were absent in D58 isolate. The F gene has transmembrane domains at positions 15-25 and 501-523 and glycosylation sites at 85, 191, 366, 447, 471 and 541. The fusion protein cleavage site has amino acids GRQGRL and fusion peptide starts with leucine. The HN and F genes have 26 and 23 B cell epitopes respectively. The D58 isolate was grouped with less virulent strains in phylogenetic analysis.
PDF References Citation
How to cite this article
P. Ponnusamy, J. John Kirubaharan and Andrew Chandramohan, 2009. Sequence Analysis of HN and F Genes of a less Virulent Newcastle Disease Virus Isolated from Unvaccinated Village Chicken. International Journal of Poultry Science, 8: 985-994.
DOI: 10.3923/ijps.2009.985.994
URL: https://scialert.net/abstract/?doi=ijps.2009.985.994
DOI: 10.3923/ijps.2009.985.994
URL: https://scialert.net/abstract/?doi=ijps.2009.985.994
REFERENCES
- Ananth, R., J. John-Kirubaharan, M.L.M. Priyadarshini and A. Albert, 2008. Isolation of newcastle disease viruses of high virulence in unvaccinated healthy village chickens in South India. Int. J. Poult. Sci., 7: 368-373.
CrossRefDirect Link - Bates, P.A. and M.J. Sternberg, 1999. Model building by comparison at CASP3: Using expert knowledge and computer automation. Proteins, 3: 47-54.
Direct Link - Buckland, R., E. Malvoisin and P. Beauverger, 1992. A leucine zipper structure present in the measles virus fusion protein is not required for its tetramerization but is essential for fusion. J. General Virol., 73: 1703-1707.
Direct Link - Contreras-Moreira, B. and P.A. Bates, 2002. Domain fishing: A first step in protein comparative modelling. Bioinformatics, 18: 1141-1142.
Direct Link - Cserzo, M., E. Wallin, I. Simon, G. von Heijne and A. Elofsson, 1997. Prediction of transmembrane alpha-helices in procariotic membrane proteins: The dense alignment surface method. Prot. Eng., 10: 673-676.
Direct Link - Deng, R., A.M. Mirza, P.J. Mahon and R.M. Iorio, 1997. Functional chimeric HN glycoproteins derived from Newcastle disease virus and human parainfluenza virus-3. Arch. Virol. Suppl., 13: 115-130.
Direct Link - Gravel, A.K. and T.G. Morrison, 2003. Interacting domains of HN and F genes of Newcastle disease viruses. J. Virol., 77: 11040-11049.
Direct Link - Huang, Z., S. Krishnamurthy, A. Panda and S.K. Samal, 2003. Newcastle disease virus V protein is associated with viral pathogenesis and functions as an alpha interferon antagonist. J. Virol., 77: 8676-8685.
CrossRefDirect Link - Lei-Tao, T., X. Huai-Ying, W. You-Ling, Q. Zhuo-Ming, L. Sun and C. Zhi-Zhong, 2008. Molecular characterization of 3 new virulent Newcastle disease virus variants isolated in China. J. Clin. Microbiol., 46: 750-753.
Direct Link - Yu, L., Z. Wang, Y. Jiang, L. Chang and J. Kwank, 2001. Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan. J. Clin. Microbiol., 39: 3512-3519.
CrossRefPubMedDirect Link - McGinnes, L.W., K. Gravel and T.G. Morrison, 2002. Newcastle disease virus HN protein alters the conformation of the F protein at cell surfaces. J. Virol., 76: 12622-12633.
CrossRefDirect Link - Romer-Oberdorfer, A., O. Werner, J. Veits, T. Mebatsion and T.C. Mettenleiter, 2003. Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J. General Virol., 84: 3121-3129.
CrossRefDirect Link - Olav, S., O.S. Deleeuw, G.L. Hantog, N. Ravenshost and B.P. Peeters, 2005. Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein. J. Gen. Virol., 86: 1759-1769.
CrossRefDirect Link - Peeters, B.P., O.S. Deleeuw, I. Verstigen, G. Koch and A.L. Gielkens, 2001. Generation of a recombinant chimeric Newcastle disease virus vaccine that allows serological differentiation between vaccinated and infected animals. Vaccine, 19: 1616-1627.
Direct Link - Pitt, J.J., E. da Silva and J. Gorman, 2000. Determination of the disulfide bond arrangement of Newcastle disease virus Haemagglutinin-neuraminidase Correlation with a beta-sheet propeller structural fold predicted for Paramyxoviridae attachment proteins. J. Biol. Chem., 275: 6469-6478.
Direct Link - Reitter, J., T. Sergel and T.G. Morrison, 1995. Mutational analysis of the leucine zipper motif in the Newcastle disease virus fusion protein. J. Virol., 69: 5995-6004.
Direct Link - Sakaguchi, M., H. Nakamura, K. Sonoda, F. Hamada and K. Hirai, 1996. Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine, 14: 747-752.
CrossRefDirect Link - Sakaguchi, T., T. Toyoda, B. Gotoh, N.M. Inocencio, K. Kuma, T. Miyata and Y. Nagai, 1989. Newcastle disease virus evolution I Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. Virology, 169: 260-272.
Direct Link - Tan, W.S., C.H. Lau, B.K. Ng, A.L. Ibrahim and K. Yusoff, 1995. Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease virus. DNA Sequencing, 6: 47-50.
Direct Link