Adriana Kolesarova
Department of Animal Physiology, Slovak University of Agriculture in Nitra,Tr. A. Hlinku 2, SK-949 76, Slovak Republic
M. Capcarova
Department of Animal Physiology, Slovak University of Agriculture in Nitra,Tr. A. Hlinku 2, SK-949 76, Slovak Republic
A. Sirotkin
Department of Genetics and Reproduction,Animal Production Research Centre, Nitra, 95141, Luzianky, Slovak Republic
P. Massanyi
Department of Animal Physiology, Slovak University of Agriculture in Nitra,Tr. A. Hlinku 2, SK-949 76, Slovak Republic
ABSTRACT
The general objective of this in vitro study was to examine the secretory activity of ovarian granulosa cells of hens after lead (Pb) and molybdenum (Mo) administrations. In this study the effect of various doses of Pb and Mo on the insulin-like growth factor-I (IGF-I) and progesterone (P4) release by ovarian granulosa cells was demonstrated. Ovarian granulosa cells were incubated with lead acetate/ammonium molybdate- group A 0.09 mg/mL, group B 0.17 mg/mL, group C 0.33 mg/mL and the control group without Pb and Mo administrations for 18 h. The IGF-I and progesterone release by granulosa cells of hens was assessed by RIA. It was demonstrated that isolated ovarian cells were able to survive in culture and release hormones IGF-I and P4 after experimental Pb and Mo administrations. Our observations demonstrated that the secretory activities of granulosa cells of hens were dependent on Pb and Mo doses. Lower Pb concentrations (0.09 mg/mL and 0.17 mg/mL) induced IGF-I release but higher dose (0.33 mg/mL) stimulated P4 release by ovarian granulosa cells of hens. Higher Mo concentration (0.33 mg/mL) reduced of IGF-I release while doses 0.17 mg/mL and 0.33 mg/mL stimulated P4 release by ovarian cells of hens. These findings contribute to our knowledge of the effect of lead and molybdenum on ovarian function of hens.
PDF References Citation
How to cite this article
Adriana Kolesarova, M. Capcarova, A. Sirotkin and P. Massanyi, 2009. Insulin-Like Growth Factor-I and Progesterone Release by Ovarian Granulosa Cells of Hens after Experimental Lead and Molybdenum Administrations in vitro. International Journal of Poultry Science, 8: 890-895.
DOI: 10.3923/ijps.2009.890.895
URL: https://scialert.net/abstract/?doi=ijps.2009.890.895
DOI: 10.3923/ijps.2009.890.895
URL: https://scialert.net/abstract/?doi=ijps.2009.890.895
REFERENCES
- Armstrong, D.G. and C.O. Hogg, 1996. Insulin-like growth factor I (IGF-I), IGF-II and type-I IGF receptor gene expression in the ovary of the laying hen. J. Reprod. Fert., 106: 101-106.
Direct Link - Arnhold, I.J., A. Lofrano-Porto and A.C. Latronico, 2009. Inactivating mutations of luteinizing hormone beta-subunit or luteinizing hormone receptor cause oligo-amenorrhea and infertility in women. Hormone Res., 71: 75-82.
Direct Link - Arpasova, H., N. Lukac, P. Massanyi, A. Kalafova, M. Capcarova, J. Kovacik and G. Formicki, 2007. Nickel induced alternation of hen body weight, egg production and egg quality after an experimental peroral administration. J. Environ. Sci. Health Part B, 42: 913-918.
Direct Link - Arpasova, H., V. Petrovic, M. Mellen, M. Kacaniova, K. Cobanova and L. Leng, 2009. The effects of supplementing sodium selenite and selenized yeast to the diet for laying hens on the quality and mineral content of eggs. J. Anim. Feed Sci., 18: 90-100.
Direct Link - Bersenyi, A., E. Berta, I. Kadar, R. Glavits, M. Szilagyi and S.G. Fekete, 2008. Effects of high dietary molybdenum in rabbits. Acta Vet. Hung., 56: 41-55.
Direct Link - Bires, J., I. Maracek, P. Bartko, M. Biresova and T. Weissova, 1995. Accumulation of trace elements in sheep and the effects upon qualitative and quantitative ovarian changes. Vet. Hum. Toxicol., 37: 349-356.
Direct Link - Capcarova, M., A. Kolesarova, H. Arpasova, P. Massanyi and N. Lukac et al., 2008. Blood biochemical dynamics and correlations in laying hens after experimental nickel administration. Int. J. Poult. Sci., 7: 538-547.
CrossRefDirect Link - Fortoul, T.I., C.R. Salgado, S.G. Moncada, I.G. Sanchez, I.E. Lopez and G. Espejel, 1999. Ultrastructural findings in the murine nonciliated bronchiolar cells (NCBC) after subacute inhalation of lead acetate. Acta Vet. Brno, 68: 51-55.
Direct Link - Grado-Ahuir, J.A., P.Y. Aad, G. Ranzenigo, F. Caloni, F. Cremonesi and L.J. Spicer, 2009. Microarray analysis of insulin-like growth factor-I-induced changes in messenger ribonucleic acid expression in cultured porcine granulosa cells: Possible role of insulin-like growth factor-I in angiogenesis. J. Anim. Sci., 87: 1921-1933.
CrossRef - Grasselli, F., G. Basini, S. Bussolati and F. Bianco, 2005. Cobalt chloride, a hypoxia-mimicking agent, modulates redox status and functional parameters of cultured swine granulosa cells. Reprod. Fert. Dev., 17: 715-720.
Direct Link - Haywood, S., Z. Dincer, B. Jasani and M.J. Loughran, 2004. Molybdenum-associated pituitary endocrinopathy in sheep treated with ammonium tetrathiomolybdate. J. Comp. Pathol., 130: 21-31.
CrossRefDirect Link - Henson, M.C. and P.J. Chedrese, 2004. Endocrine disruption by cadmium, a common environmental toxicant with paradoxical effects on reproduction. Exp. Biol. Med., 229: 383-392.
CrossRefDirect Link - Iavicoli, I., G. Carelli, E.J. Stanek, N. Castellino and E.J. Calabrese, 2004. Effects of low doses of dietary lead on puberty onset in female mice. Reprod. Toxicol., 19: 35-41.
Direct Link - Ishaq, M., M.A. Khan, F.A. Jan and I. Ahmad, 2009. Heavy metals in brick kiln located area using atomic absorption spectrophotometer: A case study from the city of Peshawar, Pakistan. Environ. Monit. Assess.
Direct Link - Järup, L., 2003. Hazards of heavy metal contamination. Br. Med. Bull., 68: 167-182.
CrossRefDirect Link - Mendel, R.R. and F. Bittner, 2006. Cell biology of molybdenum. Biochim. Biophys. Acta, 1763: 621-635.
Direct Link - Mlynarcikova, A., M. Fickova and S. Scsukova, 2005. Ovarian intrafollicular processes as a target for cigarette smoke components and selected environmental reproductive disruptors. Endocr. Regul., 39: 21-32.
Direct Link - Parry, N.M., M. Phillippo, M.D. Reid, B.A. McGaw, D.J. Flint and N. Loveridge, 1993. Molybdenum-induced changes in the epiphyseal growth plate. Calcif Tissue Int., 53: 180-186.
Direct Link - Pine, M.D., J.K. Hiney, R.K. Dearth, G.R. Bralton and W.L. Dees, 2006. IGF-1 administration to prepubertal female rats can overcome delayed puberty caused by maternal Pb exposure. Reprod. Toxicol., 21: 104-109.
Direct Link - Priya, P.N., A. Pillai and S. Gupta, 2004. Effect of simultaneous exposure to lead and cadmium on gonadotropin binding and steroidogenesis on granulosa cells: An in vitro study. Indian J. Exp. Biol., 42: 143-148.
Direct Link - Sirotkin, A.V. and R. Grossmann, 2007. The role of ghrelin and some intracellular mechanisms in controlling the secretory activity of chicken ovarian cells. Comp. Physiol. A Mol. Integrat. Physiol., 147: 239-246.
Direct Link - Sirotkin, A.V., R. Grossmann, M.T. Maria-Peon, J. Roa, M. Tena-Sempere and S. Klein, 2006. Novel expression and functional role of ghrelin in chicken ovary. Mol. Cell Endocrinol., 257: 15-25.
CrossRefPubMedDirect Link - Tosca, L., C. Chabrolle, S. Crochet, S. Tesseraud and J. Dupont, 2008. IGF-1 receptor signaling pathways and effects of AMPK activation on IGF-1-induced progesterone secretion in hen granulosa cells. Domestic Anim. Endocrinol., 34: 204-216.
Direct Link - Uzumcu, M. and R. Zachow, 2007. Developmental exposure to environmental endocrine disruptors: Consequences within the ovary and on female reproductive function. Reprod. Toxicol., 23: 337-352.
Direct Link - Wang, C., Y. Zhang, J. Liang, G. Shan, Y. Wang and Q. Shi, 2006. Impacts of ascorbic acid and thiamine supplementation at different concentrations on lead toxicity in testis. Clin. Chim. Acta, 370: 82-88.
Direct Link