M. de Beer
University of Arkansas, Center of Excellence for Poultry Science,Fayetteville, AR 72701, USA
C. N. Coon
University of Arkansas, Center of Excellence for Poultry Science,Fayetteville, AR 72701, USA
ABSTRACT
Two experiments were conducted to determine effects of Everyday (ED) or Skip-a-day (SK) feeding and dietary L-carnitine on lipid metabolism and stress in broiler breeders. In Experiment 1 a 2x2 factorial design was used to compare feeding regimens (ED vs SK) and L-carnitine supplementation (0 vs 50 mg/kg). L-carnitine supplementation began at d 1 and lasted throughout the 45 weeks experimental period. SK programs were implemented from 28 days of age to 5% production. Parameters measured included in vitro Lipogenesis (IVL), Heterophil/Lymphocyte ratio (H/L) and yolk IgY content. Liver and blood samples were taken 1 h after feeding, at various intervals during the rearing and production periods. Both SK feeding and L-carnitine increased liver wts during rearing but differences dissipated after onset of lay. Part of the increase in liver weight in SK birds was due to higher lipid contents. L-carnitine tended to reduce liver lipid during rearing. IVL was increased by SK feeding during the rearing period. L-carnitine and SK feeding interacted to increase IVL at 20, 22 and 27 weeks. H/L was elevated at 7 weeks in SK birds, but no differences were observed after that. Neither L-carnitine nor feeding regimens affected maternal IgY transfer to egg yolks. In Experiment 2, the same effects were tested but a low density grower diet was used from 4-18 weeks. The grower diet had 9% less energy and 7% less protein than in experiment 1. Liver wt was increased in SK and L-carnitine supplemented birds up to 20 weeks. By 40 weeks, ED birds had higher liver weights than SK. Liver fat was generally higher in SK birds than ED during rearing. SK feeding increased IVL but unlike Experiment 1, L-carnitine did not. H/L ratio was elevated in SK up to 20 weeks of age after which no differences occurred. L-carnitine did not affect H/L. In conclusion, feeding regimens and L-carnitine can alter hepatic lipid synthesis. Feeding regimens like SK, incorporating lengthy periods without feed can result in elevated H/L ratios but birds are generally able to adapt to such regimens over time
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How to cite this article
M. de Beer and C. N. Coon, 2009. The Effect of Different Feed Restriction Programs and Dietary L-Carnitine
Supplementation on Hepatic Lipogenesis, Plasma Heterophil to Lymphocyte Ratio
and Yolk IgY Content of Broiler Breeder Hens. International Journal of Poultry Science, 8: 328-341.
DOI: 10.3923/ijps.2009.328.341
URL: https://scialert.net/abstract/?doi=ijps.2009.328.341
DOI: 10.3923/ijps.2009.328.341
URL: https://scialert.net/abstract/?doi=ijps.2009.328.341
REFERENCES
- Akiba, Y., L.S. Jensen and C.X. Mendonca, 1983. Laboratory model wuth chicks for assay of nutritional factors affecting hepatic lipid accumulation in laying hens. Poult. Sci., 62: 143-151.
PubMedDirect Link - Bollen, L.S. and J. Hau, 1997. Immunoglobulin G in the developing oocytes of the domestic hen and immunospecific antibody response in serum and corresponding egg yolk. In Vivo, 11: 395-398.
PubMedDirect Link - Buyse, J., G.P.J. Janssens and E. Decuypere, 2001. The effects of dietary L-carnitine supplementation on the performance, organ weights and circulating hormone and metabolite concentrations of broiler chickens reared under a normal or low temperature schedule. Br. Poult. Sci., 42: 230-241.
CrossRefPubMedDirect Link - Craig, S.R. and D.M. Gatlin, 1998. Effects of carnitine and lysine on weight gain, body composition and lipogenic enzyme activity in juvenile red drum (Sciaenops ocellatus). Proceedings of the 8th International Symposium on Nutrition and Feeding of Fish, (ISNFF`98), Las Palmas de Gran Canaria, Spain, pp: 97-99.
- De Beer, M. and C.N. Coon, 2007. The effect of different feed restriction programs on reproductive performance, efficiency, frame size and uniformity in broiler breeder hens. Poult. Sci., 86: 1927-1939.
CrossRefDirect Link - De Beer, M., R.W. Rosebrough, B.A. Russell, S.M. Poch, M.P. Richards and C.N. Coon, 2007. An examination of the role of feeding regimens in reglating metabolism during the broiler breeder grower period. 1. Hepatic lipid metabolism. Poult. Sci., 86: 1726-1738.
Direct Link - De Beer, M., J.P. McMurtry, D.M. Brocht and C.N. Coon, 2008. An examination of the role of feeding regimens in regulating metabolism during the broiler breeder grower period. 2. Plasma hormones and metabolites. Poult. Sci., 87: 264-275.
Direct Link - Deng, K., C.W. Wong and J.V. Nolan, 2006. Long-term effects of early-life dietary L-carnitine on lymphoid organs and immune responses in lrghorn-type chickens. J. Anim. Phys. Anim. Nutr., 90: 81-86.
Direct Link - Dias, J., J. Arzel, G. Corraze and J. Kaushik, 2001. Effects of dietary L-carnitine supplementation on growth and lipid metabolism in European seabass (Dicentrarchus labrax). Aquac. Res., 32: 206-213.
Direct Link - Du, R., J. Qin, J. Wang, Q. Pang, C. Zhang and J. Jiang, 2005. Effect of supplementary dietary L-carnitine and yeast chromium on lipid metabolism of laying hens. Asian-Aust. J. Anim. Sci., 18: 235-240.
Direct Link - Dyck, J.R., A.J. Barr, R.L. Barr and P.E. Kolattukudy, 1998. Characterization of cardiac malonyl-CoA decarboxylase and its putative role in regulating fatty acid oxidation. Am. J. Physiol. Heart Cric. Physiol., 44: H2122-H2129.
Direct Link - Fattori, T.R., H.R. Wilson, R.H. Harms and R.D. Miles, 1991. Response of broiler breeder females to feed restriction below recommended levels. 1. Growth and reproductive performance. Poult. Sci., 70: 26-36.
CrossRefPubMedDirect Link - Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
CrossRefPubMedDirect Link - Friedman, S. and G. Fraenkel, 1955. Reversible enzymatic acetylation of carnitine. Arch. Biochem. Biophys., 59: 491-501.
PubMedDirect Link - Gross, W.B. and H.S. Siegel, 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis., 27: 972-979.
CrossRefPubMedDirect Link - Gross, W.B. and P.B. Siegel, 1986. Effect of initial and second periods of fasting on heterophil/ lymphocyte ratios and body weight. Avian Dis., 30: 345-346.
Direct Link - Hamal, K.R., S.C. Burgess, I.Y. Pevzner and G.F. Erf, 2006. Maternal antibody transfer from dams to their egg yolks, egg whites and chicks in meat lines of chickens. Poult. Sci., 85: 1364-1372.
CrossRefDirect Link - Hanks, J.H. and R.E. Wallace, 1949. Relation of oxygen and temperature in the preservation of tissues by refrigeration. Proc. Soc. Exp. Biol. Med., 71: 196-200.
PubMedDirect Link - Hermier, D., 1997. Lipoprotein metabolism and fattening in poultry. J. Nutr., 127: S805-S808.
Direct Link - Hill, J.O., S. Thacker, D. Newby, M.N. Sykes and M. Digirolamo, 1988. Influence of food restriction coupled with weight cycling on carcass energy restoration during ad-libitum refeeding. Int. J. Obesity, 12: 547-555.
PubMedDirect Link - Hillgartner, F.B., L.M. Salati and A.G. Goodridge, 1995. Physiological and molecular mechanisms involved in nutritional regulation of fatty acid synthesis. Physiol. Rev., 75: 47-76.
Direct Link - Hillgartner, F.B., T. Charron and K.A. Chesnut, 1996. Alterations in nutritional status regulate acetyl-CoA carboxylase expression in avian liver by a transcriptional mechanism. Biochem. J., 319: 263-268.
Direct Link - Hocking, P.M., M.H. Maxwell and M.A. Mitchell, 1993. Welfare assessment of broiler breeder and layer females subjected to food restriction and limited access to water during rearing. Br. Poult. Sci., 34: 443-458.
CrossRefDirect Link - Hocking, P.M., M.H. Maxwell and M.A. Mitchell, 1996. Relationships between the degree of food restriction and welfare indices in broiler breeder females. Br. Poult. Sci., 37: 263-278.
CrossRefDirect Link - Hocking, P.M., M.H. Maxwell, G.W. Robertson and M.A. Mitchell, 2001. Welfare assessment of modified rearing programs for broiler breeders. Br. Poult. Sci., 42: 424-432.
CrossRefDirect Link - Hocking, P.M., D. Waddington, M.A. Walker and A.B. Gilbert, 1987. Ovarian follicular structure of white leghorns fed ad libitum and dwarf and normal broiler breeders fed ad libitum or restricted to point of lay. Br. Poult. Sci., 28: 493-506.
CrossRefDirect Link - Hocking, P.M., D. Waddington, M.A. Walker and A.B. Gilbert, 1989. Control of the development of the ovarian follicular hierarchy in broiler breeder pullets by food restriction during rearing. Br. Poult. Sci., 30: 161-174.
CrossRefDirect Link - Katanbaf, M.N., E.A. Dunnington and P.B. Siegel, 1989. Restricted feeding in early and late feathering chickens. 3. Organ size and carcass composition. Poult. Sci., 68: 359-368.
PubMedDirect Link - Katanbaf, M.N., E.A. Dunnington and P.B. Siegel, 1989. Restricted feeding in early and late-feathering chickens. 1. Growth and physiological responses. Poult. Sci., 68: 344-351.
CrossRefDirect Link - Leslie, G.A. and W.L. Clem, 1969. Phylogeny of immunoglobulin structure and function. III. Immunoglobulins of the chicken. J. Exp. Med., 130: 1337-1352.
CrossRefPubMedDirect Link - Leveille, G.A., 1966. Glycogen metabolism in meal-fed rats and chicks and the time sequence of lipogenic and enzymatic adaptive changes. J. Nutr., 90: 449-460.
Direct Link - Lien, T.F. and Y.M. Horng, 2001. The effect of supplementary dietary L-carnitine on the growth performance, serum component, carcass traits and enzyme activities in relation to fatty acid β-oxidation of broiler chicks. Br. Poult. Sci., 42: 92-95.
Direct Link - Mast, J., J. Buyse and B.M. Goddeeris, 2000. Dietary L-carnitine supplementation increases antigen-specific immunoglobulin G production in broiler chickens. Br. J. Nutr., 83: 161-166.
Direct Link - McDaniel, G.R., J. Brake and R.D. Bushlong, 1981. Factors affecting broiler breeder performance. 1. Relationship of daily feed intake level to reproductive performance of pullets. Poult. Sci., 60: 307-312.
Direct Link - Muiruri, K.L., D.R. Romsos and G.A. Leveille, 1975. Influence of meal frequency on in vivo hepatic fatty acid synthesis, lipogenic enzyme activity and glucose tolerance in the chicken. J. Nutr., 105: 963-971.
Direct Link - Hea, E.K. and G.A. Leveille, 1969. Lipid biosynthesis and transport in the domestic chick (Galus domesticus). Comp. Biochem. Physiol., 30: 149-159.
PubMedDirect Link - Son, A.P., 1990. Isolation of IgY from the yolks of eggs by a chloroform polyethylene glycol procedure. Immunol. Invest. J. Mol. Cell. Immunol., 19: 253-258.
CrossRefDirect Link - Renema, R.A. and F.E. Robinson, 2004. Defining normal: Comparison of feed restriction and full feeding of female broiler breeders. World`s Poult. Sci. J., 60: 508-522.
Direct Link - Richards, M.P., S.M., Poch, C.N. Coon, R.W. Rosebrough, C.M. Ashwell and J.P. McMurtry, 2003. Feed restriction significantly alters lipogenic gene expression in broiler chicken. J. Nutr., 133: 707-715.
Direct Link - Robinson, F.E., J.L. Wilson, M.W. Yu, G.M. Fasenko and R.T. Hardin, 1993. The relationship between body weight and reproductive efficiency in meat-type chickens. Poult. Sci., 72: 912-922.
CrossRefDirect Link - Roncero, C. and A.G. Goodridge, 1992. Regulation of the malic enzyme and fatty acid synthase genes in chick embryo hepatocytes in culture: Corticosterone and carnitine regulate responsiveness to triiodothyronine. Arch. Biochem. Biophys., 2: 258-267.
Direct Link - Rosebrough, R.W., 2000. Dietary protein levels and the responses of broilers to single or repeated cycles of fasting and refeeding. Nutr. Res., 20: 877-886.
Direct Link - Rosebrough, R.W. and N.C. Steele, 1987. Measurement of glucose and lipid metabolism in avian liver explants. Comp. Biochem. Physiol., 88: 1041-1049.
PubMedDirect Link - Rosebrough, R.W., J.P. McMurtry, A.D. Mitchell and N.C. Steele, 1988. Chicken hepatic metabolism in vitro. Protein and energy relations in the broiler chicken-VI. Effect of dietary protein and energy restrictions on in vitro carbohydrate and lipid metabolism and metabolic hormone profiles. Comp. Biochem. Physiol. Part B: Comp. Biochem., 90: 311-316.
CrossRefPubMedDirect Link - Rosebrough, R.W., S.M. Poch, B.A. Russell and M.P. Richards, 2002. Dietary protein regulates in vitro lipogenesis and lipogenic gene expression in broilers. Comp. Biochem. Physiol. A: Mol. Integrat. Physiol., 132: 423-431.
CrossRef - Santulli, A. and V. D'Amelio, 1986. Effects of supplemental dietary carnitine on growth and lipid metabolism of hatchery-reared sea bass (Dicentrarchus labrax L.). Aquaculture, 59: 177-186.
Direct Link - Savory, C.J., E. Seawright and A. Watson, 1992. Stereotyped behavior in broiler breeders in relation to husbandry and opioid receptor blockade. Applied Anim. Behav. Sci., 32: 349-360.
Direct Link - Xu, Z.R., M.Q. Wang, H.X. Mao, X.A. Zhan and C.H. Hu, 2003. Effects of L-carnitine on growth performance, carcass composition and metabolism of lipids in male broilers. Poult. Sci., 82: 408-413.
CrossRefDirect Link - Yeh, Y. and G.A. Leveille, 1971. In vitro and in vivo restoration of hepatic lipogenesis in fasted chicks. J. Nutr., 101: 803-810.
Direct Link - Yeh, Y. and G.A. Leveille, 1972. Influence of intermittent fasting or protein-free feeding on lipid metabolism in young cockerels. J. Nutr., 102: 733-740.
Direct Link - Yu, M.W., F.E. Robinson, R.G. Charles and R. Weingardt, 1992. Effect of feed allowance during rearing and breeding on female broiler breeders. 2. Ovarian morphology and production. Poult. Sci., 71: 1750-1761.
PubMedDirect Link - Zammit, V.A., 1999. Carnitine acltransferases: Functional significance of subcellular distribution and membrane topology. Prog. Lipid Res., 38: 199-224.
PubMedDirect Link - Zuidhof, M.J., F.E. Robinson, J.J.R. Feddes, R.T. Hardin, J.L. Wilson, R.I. Mckay and M. Newcombe, 1995. The effects of nutrient dilution on the well-being and performance of female broiler breeders. Poult. Sci., 74: 441-456.
PubMedDirect Link - Zulkifli, I., E.A. Dunnington, A.S. Larsen, A. Martin and P.B. Siegel, 1993. Responses of dwarf and normal chickens to feed restriction, Eimeria tenella infection and sheep red blood cell antigen. Poult. Sci., 72: 1630-1640.
PubMedDirect Link - Kita, K., S. Kato, M. Anan Yaman, J. Okumura and H. Yokota, 2002. Dietary L-carnitine increase plasma insulin-like growth factor-I concentration in chicks fed a diet with adequate dietary protein level. Br. Poult. Sci., 43: 117-121.
CrossRef