Dafaalla M. Mekki
Department of Animal Genetics, Breeding and Reproduction,
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China 2 Department of Animal Science, Faculty of Natural Resources and Environmental Studies, Sudan
Bian Liang- yong
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
Wang Jin- yu
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
Yang Yan
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
Li lin- chuan
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
Yu Yabo
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
Hassan H. Musa
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
ABSTRACT
Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to β-actin as an internal control were used. The expression level of E2A was significantly higher (p< 0.05) in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected.
How to cite this article
Dafaalla M. Mekki, Bian Liang- yong, Wang Jin- yu, Yang Yan, Li lin- chuan, Yu Yabo and Hassan H. Musa, 2006. Differential Expression Levels Assessment of
Avian E2A Gene in Two Chinese Indigenous Duck Breeds (Anas platyrhynchos)
by Semi-Quantitative RT-PCR. International Journal of Poultry Science, 5: 895-899.
DOI: 10.3923/ijps.2006.895.899
URL: https://scialert.net/abstract/?doi=ijps.2006.895.899
DOI: 10.3923/ijps.2006.895.899
URL: https://scialert.net/abstract/?doi=ijps.2006.895.899