Dafaalla M. Mekki
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Department of Animal Science, Faculty of Natural Resources and Environmental Studies, Sudan
Bian Liang- yong
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Wang Jin- yu
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Yang Yan
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Yu Yabo
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
Hassan H. Musa
Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
ABSTRACT
Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to beta-actin as an internal control were used. The expression level of E2A was significantly (p< 0.05) higher in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected. The association between E2A relative expression level and body weight was found to be positive and moderate for the genetically lean breed (Gao you), while it was negative and has low values for genetically heavy breed (Kun shan).
How to cite this article
Dafaalla M. Mekki, Bian Liang- yong, Wang Jin- yu, Yang Yan, Yu Yabo and Hassan H. Musa, 2006. Avian E2A Expression Level Variation Associated
with Phenotypic Variation of Two Chinese Indigenous Duck Breeds, (Anas
platyrhynchos). International Journal of Poultry Science, 5: 1023-1028.
DOI: 10.3923/ijps.2006.1023.1028
URL: https://scialert.net/abstract/?doi=ijps.2006.1023.1028
DOI: 10.3923/ijps.2006.1023.1028
URL: https://scialert.net/abstract/?doi=ijps.2006.1023.1028