Mohsen Amini
Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Morteza Pirali- Hamedani
Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Hossein Ali Ebrahimi
Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Majid Darabi
Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Abbas Shafiee
Pharmaceutical Sciences Research Center, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
ABSTRACT
A HPLC method for determination of rofecoxib in human serum was presented. The method is based on pre-column derivatization of analyte to a phenanthrene derivative of drug. Rofecoxib and internal standard were extracted from serum using liquid-liquid extraction. Upon exposure to UV light, drug was found to undergo a phtocyclization reaction giving a species with high absorbance. Validation of the method has been studied in the concentration range of 2-100 ng mL-1.
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How to cite this article
Mohsen Amini, Morteza Pirali- Hamedani, Hossein Ali Ebrahimi, Majid Darabi and Abbas Shafiee, 2005. Determination of Rofecoxib in Serum with Pre-column Derivatization and Fluorescence
Detection. International Journal of Pharmacology, 1: 332-335.
DOI: 10.3923/ijp.2005.332.335
URL: https://scialert.net/abstract/?doi=ijp.2005.332.335
DOI: 10.3923/ijp.2005.332.335
URL: https://scialert.net/abstract/?doi=ijp.2005.332.335