Wilson Abrao Saad
Basic Institute of Biosciences, UNITAU, Taubate, SP, Brazil
Department of Exact and Natural Science, UNIARA, Araraquara SP Brazil, Department of Physiology and Pathology, School of Dentistry
Laura Izabel Gutierrez
Basic Institute of Biosciences, UNITAU, Taubate, SP, Brazil
Regina Celia Vendramini
Department of Physiology and Pathology, School of Dentistry,
Paulista State University, UNESP, Araraquara, SP Brazil
Andre Henrique Freiria de Oliveira
Department of Physiology and Pathology, School of Dentistry,
Paulista State University, UNESP, Araraquara, SP Brazil
Luiz Antonio de Arruda Camargo
Department of Physiology and Pathology, School of Dentistry,
Paulista State University, UNESP, Araraquara, SP Brazil
Gustavo Garcia
Department of Physiology and Pathology, School of Dentistry,
Paulista State University, UNESP, Araraquara, SP Brazil
ABSTRACT
Present studies have focused on the effect of intraperitonially i.p. injection of angiotensin II (ANG II) and pilocarpine on the salivary secretion, salivary amylase, saliva total proteins, saliva calcium sodium and potassium. Rats were anesthetized with urethane (1.25 g kg -1 b. wt.). The amount of saliva secretion was studied over a 5 min period. Male Holtzman rats (250-300 g) were housed in individual metabolic cages, with free access to food pellets and tap water ad libitum. The basal salivary flow after i.p saline 0.15 M NaCl as control was 17±1 mg/5 min. Angiotensin II (120 ng/0.5 mL), increased the salivary flow. The previous application of losartan (AT 1 ANG II receptors antagonist blocked the sialogogue effect of ANG II. PD123319 (AT 2ANG II receptors antagonist) blocked with smaller intensity the effect of ANG II. Animals treated with ANG II showed no change in the concentration of total protein, salivary amylase and calcium. The same happened when treated with losartan or PD123319. ANG II increased the concentration of sodium and decreased the concentration of potassium. Losartan blocked the effect of ANG II on sodium and potassium concentration. Pilocarpine increased the salivary flow. Losartan and PD123319 produced no change in salivary flow stimulated by pilocarpine. Pilocarpine did not alter the total protein, salivary amylase and calcium. Previous application of losartan and PD123319 produced no change in this pilocarpine effect. Pilocarpine decreased sodium and potassium concentration. Losartan and PD123319 produced no change in these effects of pilocarpine. In conclusion the results of the present study showed the importance of ANG II and pilocarpine in the control of the mechanism of salivary secretion and in the sodium and potassium-saliva concentration.
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How to cite this article
Wilson Abrao Saad, Laura Izabel Gutierrez, Regina Celia Vendramini, Andre Henrique Freiria de Oliveira, Luiz Antonio de Arruda Camargo and Gustavo Garcia, 2005. Effect of Pilocarpine and Angiotensin II on Salivary Flow, Total Protein and Electrolyte Concentrations of Saliva. International Journal of Pharmacology, 1: 190-194.
DOI: 10.3923/ijp.2005.190.194
URL: https://scialert.net/abstract/?doi=ijp.2005.190.194
DOI: 10.3923/ijp.2005.190.194
URL: https://scialert.net/abstract/?doi=ijp.2005.190.194
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