Paola Sacerdote
Department of Pharmacology, University of Milan, via Vanvitelli 32, 20129 Milano, Italy
Mauro Bianchi
Department of Pharmacology, University of Milan, via Vanvitelli 32, 20129 Milano, Italy
ABSTRACT
It has been established that nonsteroidal anti-inflammatory drugs (NSAIDs) have additional mechanisms of action, besides the inhibition of prostanoid production. We evaluated the ability of lornoxicam, a novel NSAID of the oxicam family, to interfere with the migration of human polymorphonuclear cells (PMN) in vitro. PMN were obtained from healthy donors. Chemotaxis was assessed by a Boyden microchemotaxis chamber and was stimulated with the bacterial chemoattractant formyl-met-leu-phe (fMLP, 0.1 μM), the chemokine Interleukin-8 (25 ng mL -1, 0.003 μM) or the neuropeptide substance P (0.1 μM). Lornoxicam was used at the concentrations of 0.01, 1.0 and 100 μM. This NSAID inhibited fMLP and Substance P chemotaxis starting from the concentration of 1.0 μM, whereas Interleukin-8-induced chemotaxis was significantly reduced also at 0.01 μM. As these concentrations can be easily reached in plasma and in the synovial fluid after the administration of this drug in vivo, present data suggest that the inhibition of neutrophil migration is involved in the anti-inflammatory action of lornoxicam.
PDF References
How to cite this article
Paola Sacerdote and Mauro Bianchi, 2005. Lornoxicam Inhibits Human Polymorphonuclear Cell Migration Induced by fMLP, Interleukin-8 and Substance P. International Journal of Pharmacology, 1: 180-184.
DOI: 10.3923/ijp.2005.180.184
URL: https://scialert.net/abstract/?doi=ijp.2005.180.184
DOI: 10.3923/ijp.2005.180.184
URL: https://scialert.net/abstract/?doi=ijp.2005.180.184
REFERENCES
- Ben-Baruch, A., D.F. Michiel and J.J. Oppenheim, 1995. Signals and receptors involved in recruitment of inflammatory cells. J. Biol. Chem., 270: 11703-11706.
PubMed - Diaz-Gonzales, F. and F. Sanchez-Madrid, 1998. Inhibition of leukocyte adhesion: An alternative mechanism of action of anti-inflammatory drugs. Immunol. Today, 4: 169-172.
Direct Link - Visalyaputra, S., V. Sanansilp, N. Pechpaisit, R. Choavarartana and S. Sritisarn et al., 2002. Post operative analgesic effect of intravenous lornoxicam and morphine with pre-emptive ropivacaine skin infiltration and preperitoneal instillation after transabdominal hysterectomy. J. Med. Assoc. Thai, 3: 1010-1016.
- Sacerdote, P., M. Carrabba, A. Galante, R. Pisati, B. Manfredi and A.E. Panerai, 1995. Plasma and synovial fluid interleukin-1, interleukin-6 and substance P concentrations in rheumatoid arthritis patients: Effect of the non steroidal anti inflammatory drugs indomethacin, diclofenac and naproxen. Inflammat. Res., 44: 486-490.
- Bianchi, M.M., Broggini, P. Balzarini, E. Baratelli, P. Ferrario, A.E. Panerai and P. Sacerdote, 2003. Effects of tramadol on synovial fluid concentrations of substance P and Interleukin-6 in patients with knee osteoarthritis: Comparison with paracetamol. Int. Immunopharmacol., 3: 1901-1908.
CrossRef