Nighat Aslam
Molecular Biochemistry Laboratory, Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
Farooq Latif
National Institute for Biotechnology and Genetic Engineering, Jhang Road, Faisalabad, Pakistan
Sibtain Ahmed
Molecular Biochemistry Laboratory, Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
Amer Jamil
Molecular Biochemistry Laboratory, Department of Chemistry, University of Agriculture, Faisalabad, Pakistan
ABSTRACT
Trichoderma harzianum (E-58) was grown on Vogel`s medium containing glucose and xylan as carbon source. Glucose repressed the β-glucosidase production while xylan produced the enzyme in substantial amounts. Total RNA was isolated from mycelia of Trichoderma harzianum grown in medium containing xylan as carbon source. RT-PCR was performed on the isolated RNA. cDNA strand was synthesized by using oligo (dT)18 primer. Specific primers were used to amplify bgl gene from cDNA. The bgl gene was ligated at SmaI site of pUC18 and transformed into E. coli.
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How to cite this article
Nighat Aslam, Farooq Latif, Sibtain Ahmed and Amer Jamil, 2004. Molecular Cloning of β-glucosidase Gene from Trichoderma harzianum. Biotechnology, 3: 63-66.
DOI: 10.3923/biotech.2004.63.66
URL: https://scialert.net/abstract/?doi=biotech.2004.63.66
DOI: 10.3923/biotech.2004.63.66
URL: https://scialert.net/abstract/?doi=biotech.2004.63.66
REFERENCES
- Stotz, H.U., B.R. Pittendrigh, J. Kroymann, K. Weniger, J. Fritsche, A. Bauke and T. Mitchell-Olds, 2000. Induced plant defense responses against chewing insects. Ethylene signaling reduces resistance of Arabidopsis against Egyptian cotton worm but not diamondback moth. Plant Physiol., 124: 1007-1017.
- Esterbauer, H., W. Steiner, I. Labudova, A. Hermann and M. Hayn, 1991. Production of Trichoderma cellulase in Laboratory and pilot scale. Bioresour. Technol., 36: 51-56.
CrossRefDirect Link - Mandels, M. and D. Sternberg, 1976. Recent advances in cellulase technology. J. Ferment. Technol., 54: 267-286.
Direct Link - Ikram-ul-Haq, S. Khurshid, S. Ali, H. Ashraf, M.A. Qadeer and M.I. Rajoka, 2000. Mutation of Aspergillus niger for hyperproduction of citric acid from black strap molasses. World J. Microbiol. Biotechnol., 17: 35-37.
CrossRefDirect Link - Ximenes, E.A., C.R. Felix and J. Ulhoa, 1996. Production of cellulase by Aspergillus fumigatus and characterization of one β-glucosidase. Curr. Microbiol., 32: 199-223.
Direct Link