Md. Farid Uddin
Shere e Bangla Agriculture University, Bangladesh
Shah Md. Asraful Islam
Department of Plant Pathology, Bangladesh Agriculture University, Bangladesh
Naznin Sultana
Shere e Bangla Agriculture University, Bangladesh
M.H. Kabir Shiragi
Department of Horticulture, Bangladesh Agriculture University, Bangladesh
ABSTRACT
The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh for micropropagation of tomato plant to evaluate the effect of variety and Plant Growth Regulators (PGRs) in MS medium on callus proliferation from infected tomato plant. Three tomato varieties namely, Bahar, Binatomato-2 and Binatomato-3 were used as plant materials in the present study. Virus infected young internode segments of tomato plants were cultured on MS medium supplemented with different concentrations and combinations of Plant Growth Regulators (PGRs) for callus proliferation. The combination of 0.5 mg L-1 NAA + 2.0 mg L-1 BAP in MS medium was found the best for inducing healthy and light yellow callus after 15 days culture and callus turned into yellow at 30 Days After Inoculation (DAI) in all varieties. Calli were fully virus infected which was confirmed by ELISA test. These calli can be used for the production of virus free tomato plant by shoot derived meristem culture.
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How to cite this article
Md. Farid Uddin, Shah Md. Asraful Islam, Naznin Sultana and M.H. Kabir Shiragi, 2004. Effect of Variety and Plant Growth Regulators in MS Medium on Callus Proliferation from Virus Infected Tomato Plant. Biotechnology, 3: 181-186.
DOI: 10.3923/biotech.2004.181.186
URL: https://scialert.net/abstract/?doi=biotech.2004.181.186
DOI: 10.3923/biotech.2004.181.186
URL: https://scialert.net/abstract/?doi=biotech.2004.181.186
REFERENCES
- Gomez, K.A. and A.A. Gomez, 1984. Statistical Procedures for Agricultural Research. 2nd Edn., John Wiley and Sons Inc., Hoboken, New Jersey, ISBN: 978-0-471-87092-0, Pages: 704.
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