ABSTRACT
In this experiment the screening material has been divided into three categories: I) Nine monosomic lines (1A, 1D, 2A, 2D, 3B, 4A, 4B, 5B, 7D) have shown resistance against powdery mildew, but still some plants were attacked by this disease. ii) Five monosomic lines (2B, 3D, 4D, 5D, 6A) have indicated susceptibility against this disease, but still some resistant plants were there. iii) The third group has included the monosomic line 7B, where all the plants were attacked by disease. The line 7B and the variety poros exhibited a 100 % susceptibility against powdery mildew, where all the plants (7B-119 and poros-200) tested were attacked by this disease. M30 has shown a 100 % resistance against this disease, where all the plants (200) responded positively to this disease.
PDF Abstract XML References Citation
How to cite this article
DOI: 10.3923/ajps.2003.823.825
URL: https://scialert.net/abstract/?doi=ajps.2003.823.825
INTRODUCTION
Powdery mildew, is divided into seven groups: four on cereals, hordei, tritici, avenae and secalis, and three on grasses, agropyri, bromi, and poae (Hiura, 1978). During the growing seasons the powdery mildew population is exposed to selection pressures from resistance genes in the host populations that favor the corresponding virulence genes, and from fungicides that favor fungicide-resistant powdery mildew genotypes. Studies in Europe (Limpert and Schwarzbach, 1981; Jorgensen, 1983; Wolfe, 1983) show a close association between the extent to which resistant genes and fungicides are used in cereal growing, and the frequency of corresponding virulence genes and fungicide-resistant genotypes in the powdery mildew population. Very little is known about the genetics. One of the reasons for lack of knowledge is inexpensive methods for monitoring genes for virulence and fungicide resistance are relatively new and that methods for monitoring fitness factors are yet to be developed. Moreover, the complexity of the open natural populations make it difficult to correctly interpret results. The number of chromosomes in the haploid Genome of Erysiphe graminis is unknown; it has been reported to be two and at least six. The chromosomes are very small, < 0.5 (m and therefore, difficult to detect by conventional cytological techniques (Jorgensen, 1988). Innes (1992) screened about 50 million live conidia of barley powdery mildew, but analysis of virulent mutants failed to confirm their mutational origin. In wheat powdery mildew Simmonds (1991b) reported four mutants for virulence; corresponding to three resistance genes simultaneously. Adult plant resistance of c.v. Moldwyn has been investigated very intensively. At the seedling stage the oat plants show moderately susceptibility towards powdery mildew (Jones and Hayes, 1971). In general resistance of the variety Diplomat is brought about by genes located on 14 chromosomes (Chae and Fischbech, 1979). Ellingboe (1981) reported that mildew resistance of Genesee could be traced back to one dominant gene. Sperling (1985) analyzed the resistance of M30 x Strubes Dickkopf at seedling stage inside the green house. She concluded that the resistance of plants is controlled by two independent dominant genes (15:1). Zedler (1990) reported a horizontal resistance in M30 x Carston v. Horizontal resistance in M30 and susceptibility of poros against powdery mildew is also reported by Khan and Bluethner (1994). The main objective of this project was to screen the wheat genotypes against powdery mildew at seedling stage.
MATERIALS AND METHODS
Project was conducted at the Cytogenetics Laboratory, Plant Protection Department, Martin-Luther-University, during a 3-months visit program to, Federal Republic of Germany. A wide range of techniques have been devised for studies on cereal powdery mildew, although primarily for elucidating host resistance of the gene-for-gene type.
Table 1: | Infection grade assessment scale for powdery mildew (Nover, 1941) |
Table 2: | Screening of wheat seedlings against powdery mildew inside the green-house |
%= 5% X2-3.84*Schmalz, 1989 |
In this project 120 F2 seeds/line were sown in plastic trays inside the green house. Twelve days older seedlings were inoculated by shaking heavily the diseased plants of variety Strubes Dickkopf over the seedlings (Hiura, 1978). For artificial infection the Race 2000 (Pm 2- Frauenstein et al., 1983) was used and screening of plants against the disease was done by using the assessment scale 0-4 (Table 1).
RESULTS AND DISCUSSION
As a result of artificial infection and spores inoculation the genotypes under observation have responded in three different ways: The first group being separated as a result of artificial inoculation include all those monosomic lines which carry resistant genes against powdery mildew and those lines are 1A, 1D, 2A, 2D, 3B, 4A, 4B, 5B, and 7D. The second group of plants is actually the combination of susceptible genotypes, and they are, 2B, 3D, 4D, 5D, and 6A. The third group includes only monosomic line 7B. In case of 7B 119 plants were tested against powdery mildew, which indicated 100% susceptibility to this disease. This line has got 100% similarity with variety poros, because poros is also susceptible to powdery mildew. Table 2 indicates that 140 poros plants were tested against this disease and all the plants were attacked by this disease. M30 has shown complete resistance against this disease. Similar type of results were reported by Sperling (1985). She crossed M30 with Strubus Dickkopf and concluded that two independent dominant genes (Pm 2 and Pm 6) are responsible for the resistance inheritance. These findings are in close agreement to those reported by Khan (1991), who identified chromosomes 2B, 4D, 5A, and 6A as a resistant and 1A, 2A, 3A, 4B, and 7D as susceptible to this disease. JHA (1969a) and (1969b) examined the variety Lerma Rajo and concluded that resistant genes are located on chromosome 6B in seedling and 2B, 3A, 3B, 4B and 5A in adult stage.
REFERENCES
- Ellingboe, A.H., 1981. Changing concepts in host-pathogen genetics. Annu. Rev. Phytopathol., 19: 125-143.
Direct Link - Frauenstein, K., H. Meyer, U. Walther and H. Wolfram, 1983. Neue Testsortimente und Rassenbezeichnungen bei Mehltau (New genotypes and races relationship of powdery mildew) Erysiphe graminis DC. f. sp. Tritici, hordei, secalis, Marchal) und Zwergrost (Puccinia hordei Otth). Arch. Phytopathol. Plant Prot., 19: 239-246.
- Innes, N.L., 1992. Gene banks and their contribution to the breeding of disease resistant cultivars. Euphytica, 63: 23-31.
Direct Link - Jones, I.T. and J.D. Hayes, 1971. The effect of sowing date on adult plant resistance to Erysiphe graminis f. sp. avenae in oats. Ann. Applied Biol., 68: 31-39.
Direct Link - Simmonds, N.W., 1991. Genetics of horizontal resistance to diseases of crops. Biol. Rev., 66: 189-241.
Direct Link