C. Subathra Devi
Not Available
V. Mohana Srinivasan
Not Available
ABSTRACT
Bacterial and fungal contaminants create a major problem in plant tissue culture laboratory. Once the explants have been established in vitro, it is essential that the culture to be indexed for the presence of microbial contaminants before being multiplied. Besides increased morality, the presence of latent infection can result in variable growth, tissue necrosis, reduced shoot proliferation and reduced rooting. The percentage occurrence of contaminants in initial and establishment stage of sugarcane tissue culture process was studied. Fungal and bacterial contaminants were isolated from different varieties sugarcane explants. Contaminants were more in the initial stage when compared to the establishment stage. The most predominantly occurring bacteria were Bacillus sp. Serratia sp. Pseudomonas sp. and Staphylococcus sp. and the most dominating fungal contaminants were Aspergillus, Candida, Cladosporium, Fusarium, Microsporum and Pencillum. Highest percentage occurrence was recorded for bacterial contaminants.
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How to cite this article
C. Subathra Devi and V. Mohana Srinivasan, 2006. Studies on Various Atmospheric Microorganisms Affecting the Plant Tissue Culture Explants. American Journal of Plant Physiology, 1: 205-209.
DOI: 10.3923/ajpp.2006.205.209
URL: https://scialert.net/abstract/?doi=ajpp.2006.205.209
DOI: 10.3923/ajpp.2006.205.209
URL: https://scialert.net/abstract/?doi=ajpp.2006.205.209
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