Protective Effect of Kombucha Tea on Liver Damage Induced by Thioacetamide in Rats

Journal of Biological Sciences

Volume 14 (5): 343-348, 2014

Research Article

Protective Effect of Kombucha Tea on Liver Damage Induced by Thioacetamide in Rats

Najmeh Kabiri, Mahboobeh Ahangar Darabi, Mahmoud Rafieian-Kopaei, Mahbubeh Setorki and Monir Doudi

Abstract
The aim of this study is to evaluate the possible protective effects of Kombucha tea against thioacetamide induced liver damage in rats. A total of 24 male Wistar rats were divided into four groups: Control, treated with thioacetamide (TAA) treated with TAA and then Kombucha tea, treated with Kombucha tea and then TAA; following 3 weeks of treatment. All the animals were killed and liver tissue samples were obtained for histopathological investigation. The data showed that TAA significantly increased aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) but not bilirubin. The treatment by Kombucha tea promoted a significant reduction in serum enzyme levels AST, ALT, ALP, LDH and reduction in bilirubin content. The results show that the Kombucha tea has protective effects against the thioacetamide induced hepatotoxicity that might be due to antioxidant activities of these plants.

  How to cite this article:

Najmeh Kabiri, Mahboobeh Ahangar Darabi, Mahmoud Rafieian-Kopaei, Mahbubeh Setorki and Monir Doudi, 2014. Protective Effect of Kombucha Tea on Liver Damage Induced by Thioacetamide in Rats. Journal of Biological Sciences, 14: 343-348.

DOI: 10.3923/jbs.2014.343.348

URL: https://scialert.net/abstract/?doi=jbs.2014.343.348

INTRODUCTION

Liver is the most important organ which plays a pivotal role in regulating various physiological processes in the body. The liver is involved in several vital functions in human metabolism. Therefore, any damage to the liver indicted by hepatotoxic agents is of grave consequences. Liver cirrhosis associated with various pathological processes, is characterized by progressive fibrosis producing liver injury, portal hypertension and carcinoma (Shahani, 1999). Thioacetamide (TAA) is a commonly used chemical compound to induce liver fibrosis that mimics human liver cirrhosis (Aydin et al., 2010). TAA is a typical hepatotoxin, causing centrilobular necrosis. It induces apoptosis in the rat liver based on histochemical observations (Ledda-Columbano et al., 1991).

Medicinal plants have been used from ancient times for wide variety diseases (Khosravi-Boroujeni et al., 2012; Shirzad et al., 2011; Shamsi et al., 2011) as well as for hepatotoxicity (Kazemi et al., 2010; Heidarian and Rafieian-Kopaei, 2012) and renal toxicity (Rafiean-Kopaie and Nasri, 2013; Baradaran et al., 2013; Nasri et al., 2013) induced by other drugs. Recent studies have also shown promising effects one different complications such as hypoglycemic (Behradmanesh et al., 2012), antibacterial (Bahmani et al., 2013; Sharafati-Chaleshtori et al., 2011), lipid peroxidation (Madihi et al., 2013; Heidarian et al., 2013) and cancer (Shirzad et al., 2009) prevention and these effects have mostly been attributed to their antioxidants and radical scavenging properties (Rafieian-Kopaei et al., 2013a; Rafieian-Kopaei, 2012; Setorki et al., 2012).

Kombucha tea is a traditional fermented beverage lightly sweetened effervescent drink of black tea that is produced by fermenting the tea using a symbiotic colony of yeast and bacteria (Teoh et al., 2004). Kombucha is with a history of several thousand years in the East and yet is quite popular today in the West (Teoh et al., 2004). Numerous studies refer to Kombucha’s antimicrobial properties suggests that; it might influence the gastro-intestinal microbial flora of human body (Sreeramulu et al., 2001). The beneficial properties of Kombucha tea is attributed to the presence of tea polyphenols, gluconic acid, glucuronic acid, lactic acid, vitamins, amino-acids, antibiotics and variety of micronutrients produced during fermentation. This beverage is reported to have medicinal effects against metabolic diseases, arthritis, indigestion and various types of cancer (Sreeramulu et al., 2001). Recent studies suggest that Kombucha tea prevents paracetamol induced hepatotoxicity and chromate (VI) induced oxidative stress in rats (Pauline et al., 2001). In this study the hepatoprotective effects of Kombucha tea were evaluated against TAA induced liver toxicity in Wistar rats.

MATERIALS AND METHODS

One hundred grams of sugar was added to 1 L of distilled water and the solution was boiled for 15 min in a sterile conical flask. Black tea powder (Lipton is a brand of tea) was added to the flask (12 g L-1), which was then left to cool down at room temperature for 1 h. The mixture was filtered using a sterile nylon mesh and the filtrate was used as black tea. The kombucha cultures were purchased from a Exxon Company Isfahan. The cultures were stored at 4°C prior to fermentation. Black tea was poured into 1 L glass jars, which were sterilized and inoculated with 2.5% (w/v) of freshly grown kombucha mat that had been grown and maintained in the same medium. The fermentation, kept under aseptic conditions, was carried out by incubating the kombucha culture at 28±1°C for 12 days. The flask was covered with clean cheese cloth and fixed with rubber bands. The medium (brew) was then centrifuged aseptically at 1500xg for 30 min and stored in polypropylene vials at -20°C for further use. New kombucha mat developed from the mother culture.

Wistar male rats (250-200 g b.wt.), with free access in bread and tab water in the animal house in Isfahan University of Medical Sciences were used. Animals were kept under controlled temperature (23±2°C) and 12:12-h light-dark cycle conditions. Twenty four male Wistar rats were randomly selected to designate six equal groups as follows:

Group 1:
Control group
Group 2: Injected (i.p.) with TAA (400 mg kg-1 b.wt.) for 2 weeks
Group 3: Injectd with TAA (400 mg kg-1 b.wt.) and then Kombucha tea (50 mL kg-1 each rat, for 3 weeks)
Group 4: Kombucha tea (each rat for 3 weeks) and then TAA (400 mg kg-1 b.wt.)

At the end of the experiment, the rats are killed in order to collect samples of serum and liver. The study was reviewed and approved by the Ethics Committee of Isfahan University of Medical Sciences.

Biochemical evaluations: Serum samples from the rats in groups 1 to 4 were analyzed for alanin aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol (Ch), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C) and triglyceride (TG) and were measured applying special kits (DiaSys, Germany) which utilized the colorimetric method, in an autoanalyzer (Hitachi autoanalyzer, Hitachi Co., Tokyo). The serum bilirubin was measured as well.

Histopathological examinations: Animals in each group were killed at the end of third week; the liver was removed rapidly and fixed in 10% formalin. They were then dehydrated and paraffin embedded. Two to three micron thick tissues were cut and sectioned. The sectioned tissues were routinely stained with Hematoxilin and Eosin for quantitative assessment of liver injuries. This assessment was performed a numerical scoring system based on injuries induced by TAA.

Statistical analysis: All values were expressed as Mean±SD. Significant differences among the groups were determined by one-way ANOVA using the SPSS 13.0 software package program. p<0.05 was considered as statistically significant.

RESULTS

Results are shown in Table 1. Significant decrease in body weight was observed in thioacetamide group in comparison to control group which restored in the group 4 (which received Kombucha tea and then TAA) in comparison to the group treated with thioacetamide (Table 1).

Rats treated with TAA developed significant hepatic impairment as observed from elevated serum levels of hepatospecific enzymes AST, ALT, ALP and LDH (Table 1). Serum bilirubin level was also enhanced by TAA treatment. Serum total cholesterol (Ch), HDL-cholesterol (HDL-C) and TG levels were considerably reduced and LDL-cholesterol (LDL-C) increased by TAA treatment (Table 1). Plasma AST, ALT, ALP and LDH were decreased in group 3 and 4 treated with Kombucha tea (respectively before and after TAA administration), compared to thioacetamide treated group and were even comparable with the control group regarding a reduction in bilirubin content (Table 1).

Histopathological observations: Histology of the liver sections of normal control animals (Group 1) showed normal hepatic cells with well preserved cytoplasm, prominent nucleus and nucleolus and well brought out central vein (Fig. 1). Histopathological studies demonstrated that animals in group 2 which were exposed to TAA showed focal necrosis, increased mitosis at cells, apoptosis, abnormally mitosis, inflammation at portal space and enlarged nucleus (Fig. 1). The prevention groups of kombucha tea+TAA and TAA+Kombucha tea, showed normal histology of liver sections.

Table 1: Effect of TAA, Kombucha tea treatment on rat body weight, liver weight and biochemical parameters
Group 1: Control group, Group 2: Injected (i.p.) with TAA (400 mg kg-1 b.wt.) for 2 weeks, Group 3: Injectd with TAA (400 mg kg-1 b.wt.) and then kombucha tea (for 3 weeks), Group 4: Kombucha tea (50 mL each rat, for 3 weeks) and then with TAA (400 mg kg-1 b.wt.), Body and liver weights (BW and LW) were recorded at the time of killing. ALT: Alanin aminotransferase, AST: Aspartataminotransferase, ALP: Alkalinphosphatase, LDH: Lactate dehydrogenase, Cho: Total cholesterol, LDL-C: LDL-cholesterol, HDL-C: HDL-cholesterol and TG: Triglyceride, Data is expressed as the Mean±SD, BW, Body weight, LW: Liver weight, *p>0.05 vs. controls **p>0.05 vs. TAA

Fig. 1(a-g):
Hepatoprotective effect Kombucha tea against Thioacetamide induced acute hepatotoxicity in rats. Liver sections were stained with H and E, (a) Normal, (b and c) TTA, (d and e) TAA and then kombucha tea and (f and g) kombucha tea and then TAA (Magnification 40), ba: Abnormal mitosis, bb: Cells with apoptosis, bc: Mitotic cells, bd: Inflammation around the port area

DISCUSSION

In this study the hepatoprotective effects of Kombucha tea were evaluated against TAA induced liver toxicity. TAA significantly increased AST, ALT, ALP, LDH but not bilirubin. The treatment by Kombucha tea promoted a significant reduction in serum enzyme levels (AST, ALT, ALP and LDH) and reduction in bilirubin content.

Thioacetamide is a classic hepatotoxic reagent used for liver cirrhosis induction. TAA is hepatotoxic and affects DNA, RNA, protein synthesis and glutathione content, which, in turn, induces intrahepatic metabolic changes (Ledda-Columbano et al., 1991). In this study TAA administration to rats for 10 days, other than biochemical changes, caused histopathologic changes such as necrosis, increase mitosis at cells, apoptosis, abnormally mitosis, enlarge nucleus as well as inflammation.

Free radicals are believed to play a major role in the development of TAA induced liver cirrhosis (Ledda-Columbano et al., 1991). TAA induced reduction of the number and function of mitochondria in hepatocytes of cirrhotic rats is considered to cause uncoupling in oxidative phosphorylation, leading to accumulation of NADH (Nicotinamide adenine dinucleotide) and lactate and diminishing energy synthesis rate. It is also suggested to decrease hepatic protein synthesis in cirrhosis, since most of the cell energy is used in the process (Ledda-Columbano et al., 1991). It has been shown that apoptosis caused by thioacetamide is involved the activation of caspase 3 along with extensive necrosis (Perez et al., 2004).

Different antioxidants are shown to prevent liver fibrosis and cirrhosis (Heidarian and Rafieian-Kopaei, 2013; Kazemi et al., 2011; Rafieian-Kopaei et al., 2013a). Studies have shown that diets rich in fruits and vegetables are associated with lower risk of cancer, suggesting that cancer risk can be modified/prevented by making appropriate dietary manipulations (Rafieian-Kopaei et al., 2013b).

The beneficial effects of kombucha tea are attributed to the presence of tea polyphenols, gluconic acid, glucuronic acid, lactic acid vitamins, aminoacids, antibiotics and a variety of micronutrients produced during fermentation (Vijayaraghava et al., 2000). Kombucha tea treatment, however, counteracts to the changes in mitochondrial membrane potential and prevented apoptotic cell death of the hepatocytes. Kombucha tea has the potential to ameliorate tertiary butyl hydroperoxide induced oxidative insult and cell death in murine hepatocytes more effectively than fermented black tea (Bhattacharya et al., 2011). Kombucha is a potent antioxidant proved to reduce the damage induced by oxidative stress (Bhattacharya et al., 2011). The antioxidant properties of Kombucha constituents and the protective effects of Kombucha on oxidative stress induced nephrotoxicity of Trichloroethylene treated rats have previously been documented. Kombucha tea could be used as a preventive and curative agent against carbon tetrachloride (CCl4-induced) hepatotoxicity (Gharib, 2007). Kombucha tea is rich in compounds known to be strong antioxidants and has been shown to ameliorate liver damage induced by CCl4 (Gharib, 2007). Recent studies have suggested that kombucha tea prevents paracetamol induced hepatotoxicity and chromate (VI) induced oxidative stress in rats (Pauline et al., 2001). Gluconic acid has been considered by several researchers to be the main therapeutic agent in kombucha (Loncar et al., 2000).

In this context a rise were observed in the levels of AST, ALT, ALP, LDH and bilirubin in thioacetamide treated rats. The Kombucha tea used in this study showed liver protection and maintained the structural integrity of hepatic cells. This was evident from the significant reduction in serum AST, ALT, ALP, LDH and bilirubin content. Based on the above findings it could be concluded that Kombucha tea proved a better antihepatotoxic activity against thioacetamide induced hepatic damage. More elaborate works are required to establish the exact mechanisms involved in Kombucha tea potent antihepatotoxic activities. Further experimental work is necessary to isolate and identify the active properties in the Kombucha tea which are responsible for the antihepatotoxic activities.

ACKNOWLEDGMENTS

The study has been derived from a MSc thesis and was supported in part by Grant No. 81123 from Isfahan Cardiovascular Research Center (ICRC) and partly by the Isfahan University. We would like to thank Isfahan Cardiovascular Research Center and staffs of ICRC Basic Science Laboratory.

 

References

Aydin, A.F., Z. Kusku-Kiraz, S. Dogru-Abbasoglu, M. Gulluoglu, M. Uysal and N. Kocak-Toker, 2010. Effect of carnosine against thioacetamide-induced liver cirrhosis in rat. Peptides, 31: 67-71.
CrossRef  |  PubMed  |  Direct Link  |  

Bahmani, M., N.V. Saatloo, R. Maghsoudi, H. Momtaz and K. Saki, 2013. A comparative study on the effect of ethanol extract of wild Scrophularia deserti and streptomycin on Brucellla melitensis. J. HerbMed Pharmacol., 2: 17-20.
Direct Link  |  

Baradaran, A., Y. Madihi, A. Merrikhi, M. Rafieian-Kopaei and M. Nematbakhsh et al., 2013. Nephrotoxicity of hydroalcoholic extract of Teucrium polium in Wistar rats. Pak. J. Med. Sci., 29: 329-333.

Behradmanesh, M., M. Ahmadi and M. Rafieian-Kopaei, 2012. Effect of glycogol on blood glucose level of patients with type II diabetes. Iran. J. Endocrinol. Metab., 14: 163-168.

Bhattacharya, S., P. Manna, R. Gachhui and P.C. Sil, 2011. Protective effect of kombucha tea against tertiary butyl hydroperoxide induced cytotoxicity and cell death in murine hepatocytes. Indian J. Exp. Biol., 9: 511-524.
PubMed  |  

Gharib, O.A., 2007. Does kombucha tea reduce the damage induced by radiation exposure. Egypt J. Sci. Appl., 20: 141-157.

Heidarian, E. and M. Rafieian-Kopaei, 2012. Effect of silymarin on liver phoshpatidate phosphohydrolase in hyperlipidemic rats. Biosci. Res., 9: 59-67.
Direct Link  |  

Heidarian, E. and M. Rafieian-Kopaei, 2013. Protective effect of artichoke (Cynara scolymus) leaf extract against lead toxicity in rat. Pharm. Biol., 51: 1104-1109.
CrossRef  |  

Heidarian, E., M. Rafieian-Kopaei and K. Ashrafi, 2013. The effect of hydroalcoholic extract of Allium latifolium on the liver phosphatidate phosphatase and serum lipid profile in hyperlipidemic rats. J. Babol Univ. Med. Sci., 15: 37-46.

Kazemi, S., S. Asgari, S.J. Moshtaghian, M. Rafieian-Kopaei and P. Mahzooni, 2011. Preventive effect of pumpkin (Cucurbita pepo L.) on diabetic index and histopathology of pancreas in alloxan-induced diabetes in rats. J. Isfahan Med. School, 28: 872-881.
Direct Link  |  

Kazemi, S., S. Asgary, J. Moshtaghian, M. Rafieian, A. Adelnia and F. Shamsi, 2010. Liver-protective effects of hydroalcoholic extract of Allium hirtifolium boiss. in rats with alloxan-induced diabetes mellitus. ARYA Atherosclerosis, 6: 11-15.
PubMed  |  Direct Link  |  

Khosravi-Boroujeni, H., N. Mohammadifard, N. Sarrafzadegan, F. Sajjadi and M. Maghroun et al., 2012. Potato consumption and cardiovascular disease risk factors among Iranian population. Int. J. Food Sci. Nutr., 63: 913-920.
CrossRef  |  Direct Link  |  

Ledda-Columbano, G.M., P. Coni, M. Curto, L. Giacomini and G. Faa et al., 1991. Induction of two different modes of cell death, apoptosis and necrosis, in rat liver after a single dose of thioacetamide. Am. J. Pathol., 139: 1099-1109.
PubMed  |  

Loncar, E.S., S.E. Petrovic, R.V. Malbaca and R.M. Verac, 2000. Biosynthesis of glucuronic acid by means of tea fungus. Food/Nahrung, 44: 138-139.
Direct Link  |  

Madihi, Y., A. Merrikhi, A. Baradaran, M. Rafieian-Kopaei and N. Shahinfard et al., 2013. Impact of Sumac on postprandial high-fat oxidative stress. Pak. J. Med. Sci., 29: 340-345.
CrossRef  |  

Nasri, H., M. Nematbakhsh, S. Ghobadi, R. Ansari, N. Shahinfard and M. Rafieian-Kopaei, 2013. Preventive and curative effects of ginger extract against histopathologic changes of gentamicin-induced tubular toxicity in rats. Int. J. Prev. Med., 4: 316-321.
PubMed  |  Direct Link  |  

Pauline, T., P. Dipti, B. Anju, S. Kavimani and S.K. Sharma et al., 2001. Studies on toxicity; anti-stress and hepatoprotective properties of Kombucha tea. Biomed. Environ. Sci., 14: 207-213.

Perez, M.J., F. Sanchez-Medina, M. Torres, A. Gil and A. Suarez, 2004. Dietary nucleotides enhance the liver redox state and protein synthesis in cirrhotic rats. J. Nutr., 134: 2504-2508.
Direct Link  |  

Rafiean-Kopaie, M. and H. Nasri, 2013. Ginger and diabetic nephropathy. J. Renal Injury Prev., 2: 9-10.

Rafieian-Kopaei, M., 2012. Medicinal plants and the human needs. J. HerbMed Pharmacol., 1: 1-2.
Direct Link  |  

Rafieian-Kopaei, M., A. Baradaran and M. Rafieian, 2013. Plants antioxidants: From laboratory to clinic. J. Nephropathol., 2: 152-153.
CrossRef  |  Direct Link  |  

Rafieian-Kopaei, M., A. Baradaran and M. Rafieian, 2013. Oxidative stress and the paradoxical effects of antioxidants. J. Res. Med. Sci., Vol. 18.

Setorki, M., M. Rafieian, E. Heidarian, K. Ghatreh, N. Shahinfard, R. Ansari and Z. Forouzandeh, 2012. Effect of Rhus Coriaria consumption with high cholesterol food on some atherosclerosis risk factors in rabbit. J. Babol Univ. Med. Sci., 14: 38-45.

Shahani, S., 1999. Evaluation of hepatoprotective efficacy of APCL-A poly-herbal formulation in vivo in rats. Indian Drugs, 36: 628-631.

Shamsi, F., S. Asgari, M. Rafieian, S. Kazemi and A. Adelnia, 2011. Effects of Cornus mas L. on blood glucose, insulin and histopathology of pancreas in alloxan-induced diabetic rats. J. Isfahan Med. School, 29: 929-938.
Direct Link  |  

Sharafati-Chaleshtori, R., F. Sharafati-Chaleshtori and M. Rafieian, 2011. Biological characterization of Iranian walnut (Juglans regia) leaves. Turk. J. Biol., 35: 635-639.
CrossRef  |  Direct Link  |  

Shirzad, H., F. Taji and M. Rafieian-Kopaei, 2011. Correlation between antioxidant activity of garlic extracts and WEHI-164 fibrosarcoma tumor growth in BALB/c mice. J. Med. Food, 14: 969-974.
CrossRef  |  PubMed  |  Direct Link  |  

Shirzad, H., S. Mehrdad and R.K. Mahmoud, 2009. Comparison of morphine and tramadol effects on phagocytic activity of mice peritoneal phagocytes in vivo. Int. Immunophamacol., 9: 968-970.
CrossRef  |  PubMed  |  Direct Link  |  

Sreeramulu, G., Y. Zhu and W. Knol, 2001. Characterization of antimicrobial activity in Kombucha fermentation. Acta Biotechnol., 21: 49-56.
CrossRef  |  

Teoh, A.L., G. Heard and J. Cox, 2004. Yeast ecology of Kombucha fermentation. Int. J. Food Microbiol., 95: 119-126.
CrossRef  |  

Vijayaraghava, R., M. Singh, P.V.L. Rao, R. Bhattacharya and P. Kumar et al., 2000. Subacute (90 days) oral toxicity studies of Kombucha tea. Biomed. Environ. Sci., 13: 293-299.