The mutagenic effect of colchicine to improve leaf and seed yield in two varieties of sesame (Sesamum indicum L. var. Yandev and Ex-Sudan) was investigated. Seeds of the two sesame varieties were presoaked in five different colchicine concentrations (0.1, 0.5, 1.0, 1.5 and 0.0 mM as control). The seeds were planted in a completely randomized block design for two mutant generations (M1 and M2). The M2 mutants were characterized on the basis of morphological traits such as height at maturity, anthesis period, number of leaves/plant, leaf area, internodes length, number of capsules/plant, length of capsules, number of seeds/capsule and 1000 seeds weights. The results obtained revealed highly significant difference (p≤0.01) in the morphological traits of the mutants when compared with the controls, except in the number of seeds where the effect of the mutagen is significant (p≤0.05). More so, with the exception of internodes length and 1000 seeds weight, the leaf yield parameters of the M2 mutants were found to correlate significantly with the seed yield. Similarly, colchicine was found to induce changes in the chlorophylls and carotenoids bio-syntheses among the M2 mutants, leading to the formation of five forms of chimeras as chlorinas, xanthas, striatas, virescents and lustescents. It was therefore, inferred that, colchicine-induce mutants were characterized as early flowering with tall stature having larger leaves and which produced large number of capsules with numerous seeds. The improvement of the mutants traits is concentration dependent, increases with decrease in colchicine concentration. Thus, we therefore suggested that, 0.1 mM concentration should be employed in improving sesame growth and yield related traits.
How to cite this article:
S. Nura, A.K. Adamu, S. Mu`Azu, D.B. Dangora and L.D. Fagwalawa, 2013. Morphological Characterization of Colchicine-induced Mutants in Sesame (Sesamum indicum L.). Journal of Biological Sciences, 13: 277-282.
Sesame (Sesamum indicum L.) is one of the most important oil seeds crops in the world according to some archaeological findings (Bedigian and Harlan, 1986) as ancient seeds of the crop were identified in excavations at Harappa, Pakistan dated 2000 B.C. (Uzo, 1998). It originated from Africa (Purseglove, 1968; Zeven and de Wet, 1982; Mabberly, 1997; Ashri, 1998) with the Indian sub-region serving as the secondary center of its diversity (Bedigian, 1981). A member of the family Pedaliaceae, sesame is grown all over the world for its oily seeds (Burkill, 1997) and edible leaves (Mann et al., 2003) used as vegetable. Sesame is a plant of high nutraceutical, pharmaceutical and economic importance. Sesame seeds are important source of high quality edible oil, minerals, vitamins (FAO, 1988; Pamplona-Roger, 1999; Katung and Asiribo, 2002) and proteins for poor farmers of major sesame growing countries such as Sudan, Nigeria, Ethiopia, Uganda, Mexico, Venezuela, India, China, Pakistan and Turkey (Kumar and Yadav, 2010). Besides being used in fish and beef canning, sesame oil is an important edible oil in Europe (Busari et al., 2005). The oil extracted from sesame accounts for about 2% of the world edible vegetable oil market in Europe (Busari et al., 2005). The oil substitutes olive oil and other oils in cosmetic creams and in Oleo-margarine and iodized oil (Budavari, 1996) because of its high quality for resisting oxidative rancidity due to the presence of phenylpropanoid lignans sesamin and sesamolin (Anonymous, 2005).
Even though, Nigeria is characterized by Weiss (1971) among the major sesame exporting countries, ranking second to Sudan in production and export of sesame seed (FAO, 2008); with an annual earning of over $US878 million in foreign exchange; its intensive cultivation and uses in Nigeria are not beyond subsistence level. This is attributed to the lack of high yielding varieties in conjunction with high agronomic in-puts needed for its cultivation. There is therefore, the need for improving the available germ-plasm to meet the demand of both subsistence and commercial production of the plant. Moreover, Begum and Dasgupta (2010) stressed that, several sesame genotypes are generally unimproved and many collections have been made of land races, with little or no genetic information that can lead to sesame utilization in breeding programmes. These brought about the lack of high yielding varieties compared to the high agronomic in-puts needed for sesame cultivation. Therefore, efforts need to be concerted in the improvement of sesame germplasm (Van Rheenen, 1973; Ashri, 1998). This will pave way for the genetic improvement of the crop to meet the needs of the world's growing population.
Induced mutation both in seeds and vegetatively propagated crops is one of the techniques employed in the improvement of traits of economic plants. It facilitates the isolation, identification and cloning of genes which would ultimately help in designing crops with improved yield, increased stressed tolerance and longer life span as well as reduced agronomic in-puts usage (Ahloowalia and Maluszynski, 2001). Over the years, man relied upon spontaneously occurring variants, arising from mutations to improve the yield and quality of crop plants (Herper, 1999). Effectiveness and efficiency are two distinct properties of mutagens that have been extensively discussed elsewhere (Kawai, 1986; Shah et al., 2008; Girija and Dhanavel, 2009). A number of chemicals have been found to be equally and even many times more effective and efficient mutagens (Thakur and Sethi, 1995; Kharkwal, 1998; Solanki, 2005; Rekha and Langer, 2007; Basu et al., 2008; Ganapathy et al., 2008; Wani, 2009). Therefore, this study aimed at investigating the effect of colchicine (an alkaloid derivative of Colchicum autumnale) on yield of sesame as a strategy for its improvement through chemical mutagenesis.
MATERIALS AND METHODS
The study was conducted in the Botanical Garden of the Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria (Lat11°111 N; Long 7°N 381E) in 2007 and 2008 growing season. The seeds of sesame (Sesamum indicum L. var. Yandev and Ex-Sudan) were obtained from the Jigawa State Agricultural and Rural Development Authority (JARDA) Ringim. The seeds were treated by soaking for four hours at five different treatments of colchicine concentrations including a control (0.1, 0.5, 1.0, 2.0 mM and control) and thoroughly washed in running water. The treated seeds were sown in a Completely Randomized Block Design (CRBD) with three replications for two mutant generations (M1 and M2). All cultural practices followed the protocols described in the Kano State Agricultural and Rural Development Authority (KNARDA, 2005) crop production guide. All the data obtained were subjected to analysis of variance using SAS (1988). Duncans Multiple Range Test was used to separate the means. The test of relationship was done using Pearsons product moment correlation.
The result obtained from the M2 generation analysis of variance (Table 1) indicated highly significant improvement (p≤0.01) in the effect of the mutagen on all the selected traits of sesame except in the number of seeds/capsule, where the effect is significant.
Furthermore, the result of the mean effect of colchicine on Ex-Sudan (Table 2) revealed the emergence of early flowering mutants with tall stature and substantial number of leaves (58-73 leaves/plant). The mutants leaves are larger in size (50.47-66.47 cm2) and which have longer internodes (12.87-14.53 cm).
|Table 1:||M2 generation mean squares on the effects of colchicine on some traits of sesame|
|ns: No significant difference *Significant difference (p≤0.05) **Highly significant difference (p≤0.01)|
|Table 2:||M2 generation mean effects of colchicine on some traits of ex-Sudan|
|*1Means within the columns with the same letter(s) are not significantly different at p≤0.05|
|Table 3:||M2 generation mean effects of colchicine on some traits of Yandev|
|*1Means within the columns with the same letter(s) are not significantly different at p≤0.05|
|Table 4:||Correlation co-efficients for the various traits of M2 sesame varieties treated with colchicine|
|Table 5:||Percentages of chlorophyll deficient mutants induced by colchicine in the M2 generation of sesame|
Similarly, the mutants produced high number of capsules (19-26 capsules/plant) which are larger (2.10-2.30 cm) and which produced 46-48 seeds/capsule. More so, the 1000 seeds weights of the mutants are higher than that of the control.
Similarly, the result of the mean effects of colchicine on the traits of Yandev (Table 3) showed the emergence of early flowering mutants with tall stature. The mutants produced 62-72 leaves/plant that are 47.53-59.93 cm2 in cross sectional area with a internodes length of 13.13-14.60 cm. The mutants also produced 21-24 capsules/plant that are 2.14-2.26 cm in length and which produced 43-45 seeds/capsule. More so, the mutants produced seeds that weigh higher than that of the control.
Furthermore, the leaf yield parameters of the M2 generation were found to correlate significantly with the seed yield (except between the internodes length and 1000 seeds weight where no significant correlation was found) (Table 4).
More so, the mutants were also characterized by the deficiency in chloropylls and carotenoids forming chimeras in form of chlorine, xantha, striata, virescent and lustescent as presented in Table 5.
Chemical mutagenesis has proved vital in the improvement of crop plants. The use of chemical mutagen in crop improvements initiatives have been reported in a number of species by Sander and Muchlbever (1977) and Biswas and Datta (1988). The emergence of early flowering mutants in sesame was in agreement with the work of n Rutger (1982) who reported early flowering mutants in medium grain Japonica cultivar; but was in contrast to that of Archana et al. (2004) who reported increase in the number of days required for maturity due to increased doses of mutagen treatments in Glycine max (soya beans). This may probably be due to the tendency of the mutagen to turn-on the gene responsible for inducing flowering, by making the plant to respond to environmental signals such as photoperiodism and hormonal actions as suggested by Lewis et al. (2002). Artificial induction of mutation by colchicine leads to the alteration of plant genome integrated by environmental signals as reported by Uno et al. (2001); probably by increasing the rates of cellular division and expansion at their meristematic regions. This on the other hand can be viewed as the mechanism through which colchicine-induced tall mutants emerged. This was in agreement with the findings of Hoballah (1999) who reported increased in plant heights of sesame due to irradiation mutagenesis; but was in contrast to the findings of Maluszynski et al. (2001) who reported decreased in plant height due to induced mutation in rice. The mutagen might have probably influenced the activities of cytokinin which is of paramount importance in the fundamental processes of plant development including cell division, morphogenesis as suggested by Deikman and Ulrich (1995). Sesame mutants with increased leaf number and size were induced by different colchicine concentrations. This was in agreement with the findings of Nura et al. (2011) who reported increase in leaf number and area among mutants of jute. The increased leaf number and area provides an increase in the surface area for gaseous ex-change which considerable affect the process of photosynthesis as reported by Lockhart et al. (1996). However, the increased in the internodes length among the sesame mutants is contrary to the findings of Mensah et al. (2007) who discovered internodes among colchicine-induced mutants of sesame.
Furthermore, the increase in the number of capsules and size among sesame mutants was in agreement with the work of Hoballah (1999) who reported increase in the number of capsules per plant among sesame mutants due to gamma irradiation; which considerably facilitate improvement in the sesame seeds yield. The increase in number and size of the capsules permit a substantial increase in the number of seeds produced; thereby facilitating the production of mutants producing large number of seeds. Moreover, the increase in 1000 seeds weight of sesame mutants due to colchicine treatment was in line with the work of Shen et al. (1995) who reported increase in grain weight of rice due to gamma rays in in vitro mutagenesis. This was also in conformity with the work of Pathirana et al. (2000) who reported increased in seed yield of sesame due to gamma irradiation. The results obtained in this study were therefore in agreement with those of Antoun (1980), Gautam et al. (1998); Asmahan (2000), Rascio et al. (2001) and Osama (2002), who individually reported that the improvement of yield components in various plants such as tomato, maize, rice and wheat was induced after various mutagenic treatments such as E.M.S, sodium azide and gamma rays. Moreover, the correlation between the yield and yield components of the mutants is in conformity with the findings of Akram et al. (1982) and Brar and Saini (1976) among the irradiated mutants of rice. This indicated that selection should be based on the height, leaves number and size. Beside this, induced mutation was reported by Bartley et al. (1994) to be useful in the genetic control of carotenoid bio-synthesis in certain plants like Arabidopsis thaliana, Lycopersicon esculentum, Zea mays and other species of plants. The whole range of chlorophyll mutation occurred due to the deficiency in either: chlorophylls, carotenoids or combination of both in plastid genes causing variegation as reported by Kirk and Tilney-Bassett (1978).
In conclusion, the colchicine-induced mutants of sesame were found to be characterized with advantageous traits such as increased height, leaf, capsules and seeds number as well as increased size of leaves internodes which are needed for sesame improvement. Lower colchicine concentration (0.1 mM) was found to be more beneficial and effective in improving the yield traits of sesame. We therefore, recommend the use of lower colchicine concentration in the genetic improvement of sesame.
The authors are grateful to the Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria for providing the necessary facilities and appreciate the financial assistance granted by the MacArthur Foundation, ABU, Zaria.
Ahloowalia, B.S. and M. Maluszynski, 2001. Induced mutations-A new paradigm in plant breeding. Euphytica, 118: 167-173.
Akram, M., A. Rehman and A.A. Cheeme, 1982. Correlation between yield and yield attributing characters in some induced dwarf mutants of rice (Oryza sativa L.). Pak. J. Agric. Res., 3: 141-144.
Anonymous, 2005. Importance of the oil seed (sesame) in Africa. http//www.dbedigian.com/sesame.
Antoun, S., 1980. Genetic studies in tomato (Lycopericon spp). M.Sc. Thesis, Department of Genetics, Faculty of Agriculture Ain shams University.
Archana, P., S.P. Taware and V.M. Raut, 2004. Induced variation in quantitative traits due to physical (γ rays), chemical (EMS) and combined mutagen treatments in soybean [Glycine max (L.) Merrill]. Mutation Res., 334: 49-55.
Ashri, A., 1998. Sesame Breeding. In: Plant Breeding Reviews, Janick, J. (Ed.). John Wiley and Sons, USA.
Asmahan, A.M.A., 2000. The effect of ethyl mathane sulphonate (EMS) on geneticImprovement on tomato. J. Agric. Sci., 25: 6177-6185.
Bartley, G.E., P.A. Scolnik and G. Giulio, 1994. Molecular biology of carotenoid biosynthesis in plants. Ann. Rev. Plant Physiol. Plant Mol. Biol., 45: 287-301.
Basu, S.K., S.N. Acharya and J.E. Thomas, 2008. Genetic improvement of Fenugreek (Trigonella foenum-graecum L.) through EMS induced mutation breeding for higher seed yield under Western Canada Prairie conditions. Euphytica, 160: 249-258.
Bedigian, D. and J.R. Harlan, 1986. Evidence for cultivation of sesame in the ancient world. Econ. Bot., 40: 137-154.
Bedigian, D., 1981. Origin, Diversity, Exploration and Collection of Sesame. In: Sesame: Status and Improvement, Ashri, A. (Ed.). FAO, Rome Italy, pp: 164-169.
Begum, T. and T. Dasgupta, 2010. A comparison on the effect of physical and chemical mutagenic treatments in sesame (Sesamum indicum L.). Genet. Mol. Biol., 33: 761-766.
Biswas, A.K. and A.K. Datta, 1988. Induced mutation in two Trigonella species. Bangladesh J. Bot., 17: 211-214.
Brar, G.S. and S.S. Saini, 1976. Association of grain yield and some economic characters in segregating populations of two crosses between tall and dwarf varieties of rice. Indian J. Agric. Sci., 46: 303-370.
Budavari, S., 1996. The Merck Index: An Encyclopedia of Chemicals, Drugs and Biologicals. 12th Edn., Merck and Co. Inc., Whitehous Station, New Jersey, USA.
Burkill, H.M., 1997. Pedaliaceae. In: The Useful Plants of West Tropical Africa: Families M-R, Burkill, H.M. and J.M. Dalziel (Eds.). 2nd Edn., Vol. 4, Royal Botanic Garden, Uk., pp: 414-423.
Busari, L.D., V.I.O. Olowe and A.A. Idowu, 2005. Sesame. In: Major Legumes and Oil-Seeds of Nigeria: Principles of Production and Utilization, Idem, N.U.A. and F.A. Showemimo (Eds.). Institute for Agricultural Research, ABU Zaria, Nigeria, pp: 136-167.
Deikman, J. and M. Ulrich, 1995. A novel cytokinin-resistant mutant of Arabidopsis with Abbreviated shoot development. Planta, 195: 440-449.
FAO, 1988. Traditional food plants. FAO food and nutrition paper 42. Food and Agricultural Organization, pp: 445- 450.
FAO, 2008. Sesame production. Food and Agriculture Organization of the United Nation. http://faostat.fao.org/site/567/default.aspx#ancor.
Ganapathy, S., A. Nirmalakumari, N. Senthil, J. Souframanien and T.S. Raveendran, 2008. Isolation of macromutations and mutagenic effectiveness and efficiency in Little Millet varieties. World J. Agric. Sci., 4: 483-486.
Gautam, R.K, G.S. Sethi, M.K. Rana and S.K. Shama, 1998. Induction inheritance pattern and agronomic performance of awned mutants in multiple disease resistant bread wheat cultivar. Indian J. Genet. Plant Breed, 58: 417-422.
Girija, M. and D. Dhanavel, 2009. Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combined treatments in cowpea (Vigna unguiculata L. Walp). Global J. Mol. Sci., 4: 68-75.
Herper, F., 1999. Principles of Arable Crop Production. Cambridge University Press, Cambridge, UK., pp: 50-100.
Hoballah, A.A., 1999. Selection and agronomic evaluation of induced mutant lines of sesame. Induced Mutations for Sesame Improvement IAEA-TECDOC, IAEA, Vienna, Austria, pp: 71-84.
KNARDA., 2005. Crop production guide for extension agents and farmers. Kano State Agricultural and Rural Development Authority (KNARDA), Nigeria, pp: 1-2.
Katung, P.D. and O.E. Asiribo, 2002. Correlation and path co-efficient analysis in sesame (Sesamum indicum L.). J. Trop. Bio. Sci., 2: 113-118.
Kawai, T., 1986. Radiation breeding-25 years and further on Gamma Field. Symp, 25: 1-36.
Kharkwal, M.C., 1998. Induced mutations in chickpea (Cicer arietinum L.). I. Comperative mutagenic effectiveness and efficiency of physical and chemical mutagens. Indian J. Genet., 58: 159-167.
Kirk, J.T.O. and R.A.E. Tilney-Bassett, 1978. The Plastids, their Chemistry, Structure, Growth and Inheritance. 2nd Edn., Elsevier Science Ltd., North Holland, ISBN-10: 0444800220, pp: 980.
Kumar, G. and R.S. Yadav, 2010. EMS induced genomic disorders in sesame (Sesamum indicum L.). Rom. J. Biol., 55: 97-104.
Lewis, R., D. Gaffin, M. Hoefnagels and B. Parker, 2002. Life. Mc Graw Hill, New York, USA.
Lockhart, P.J., M.A. Steel and A.W.D. Larkum, 1996. Gene duplication and the evolution of photosynthetic reaction centre proteins. FEBS Lett., 385: 193-196.
Mabberly, D.J., 1997. The Plant Book. 2nd Edn., Cambridge University Press, Cambridge, UK., Pages: 32.
Maluszynski, M., I. Szarejko, P. Barriga and A. Balcerzyk, 2001. Heterosis in crop mutant crosses and production of high yielding lines using doubled haploid systems. Euphytica, 120: 387-398.
Mann, A., M. Gbate and A.N. Umar, 2003. Medicinal and Economic Plants of Nupe land. Jube-Evans Books and Publications, Bida, Nigeria, Pages: 212.
Mensah, J.K., B.O. Obadoni, P.A. Akomeah, B. Ikhajiagbe and J. Ajibolu, 2007. The effects of sodium azide and colchicine treatments on morphological and yield traits of sesame seed (Sesame indicum L.). Afr. J. Biotechnol., 6: 534-538.
Nura, S., A.K. Adamu, S. Mu'Azu and D.B. Dangora, 2011. Chemical Mutagenesis for Improved Quality Traits in Jute (Corchorus olitorius L.). Cont. J. Biol. Sci., 4: 22-27.
Osama, M.S., 2002. Molecular genetic studies on irradiated wheat plants. Ph.D. Thesis, Department of Genetics, Faculty of Agriculture, Ain Shams University.
Pamplona-Roger, G.D., 1999. Encyclopedia of Medicinal Plants, Education and Health Library. Editorial Sofeliz, Madrid, Spain, pp: 611-612.
Pathirana, R., L.A. Weerasena and P. Bandara, 2000. Development and release of Gamma ray induced sesame mutants in Sri Lanka. Trop. Agric. Res. Ext., 3: 19-24.
Purseglove, J.W., 1968. Tropical Crops: Dicotyledons. Vol. 2, Wiley, New York, USA., pp: 430-435.
Rascio, A., M. Russo, L. Mazzucco, C. Platani, G. Nicastro and N. Di Fonzo, 2001. Enhanced osmotolerance of a wheat mutant selected for potassium accumulation. Plant Sci., 160: 441-448.
Rekha, K. and A. Langer, 2007. Induction and assessment of morpho-biochemical mutants in Artemisia pallens Bess. Genet. Resour. Crop Evol., 54: 437-443.
Rutger, J.N., 1982. Use of Induced and Spontaneous Mutations in Rice Genetics and Breeding. In: Semi-Dwarf Cereal Mutants and their Use in Cross Breeding, Kawai, T. (Ed.). International Atomic Energy Agency, Vienna, Austria, pp: 105-117.
SAS., 1988. SAS User's Guide: Statistics, Version 6.03. SAS Institute Inc., Cary, NC., USA.
Sander, C. and F.G. Muchlbever, 1977. Mutagenic effect of sodium azide and gamma irradiation in Pisum. Envl. Exp. Bot., 17: 43-47.
Shah, T.M., J.I. Mirza, M.A. Haq and B.M. Atta, 2008. Induced genetic variability in chickpea (Cicer arietinum L.). II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens. Pak. J. Bot., 40: 605-613.
Shen, Y., M. Gao, Q. Cai and Z. Liang, 1995. Isolation and genetic characterization of somaclonal mutants with large-sized grain in rice. Cereal. Res. Commun., 23: 235-241.
Solanki, I.S., 2005. Isolation of macromutations and mutagenic effectiveness and efficiency in lentil (Lens culinaris Medik.). Indian J. Genet. Pl. Breed., 65: 264-268.
Thakur, J.R. and G.S. Sethi, 1995. Comparative mutagenicity of gamma rays, ethyl methane sulphonate and sodium azide in barley (Hordeum vulgare L.). Crop. Res., 9: 350-357.
Uno, G., R. Storey and R. Moore, 2001. Principles of Botany. Mc-Graw Hill, New York, USA., pp: 1-550.
Uzo, J.O., 1998. Beniseed: A neglected oil wealth of Nigeria. Proceedings of the 1st National Workshop on Beniseed (Sesame), March 3-5, 1998, Badeggi, Nigeria, pp: 1-17.
Van Rheenen, H.A., 1973. Major problems of growing sesame (Sesamum indicum L.) in Nigeria. Bibliotheek der Landbouwhogeschool Gen. Foulkesweg la Wageningen, Netherlands, pp: 130.
Wani, A.A., 2009. Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulphonate and their combination treatments in chickpea (Cicer arietinum L.). Asian J. Plant Sci., 8: 318-321.
Weiss, E.A., 1971. Castor, Sesame and Safflower. Leanard Hill Publisher, London, UK., pp: 6110-6130.
Zeven, A.C. and J.M.J. de Wet, 1982. Dictionary of Cultivated Plants and their Regions of Diversity. Centre for Agricultural Publishing and Documentation, Wageningen, DC., USA., ISBN: 9022007855, pp: 263.