Trends in Medical Research1819-35872151-6065Science International10.3923/tmr.2007.167.175Katelysia opima]]>SomasundaramSomasundaram ThirugnanasambandanVijayabaskarPandian 4200724Glycosaminoglycan (GAG) of heparin family was localized in the tissues of bivalve Katelysia opima. Different histological staining procedures like alcian blue, aldehyde fuchsin and periodic acid-Schiffs reagent (PAS) were followed to test the presence of GAG in various tissues of this bivalve mollusk. The alcian blue with two different pH and combination of alcian blue-aldehyde fuchsin staining solutions indicated the presence of sulfated polysaccharide. The GAG was found in the tissues like gill, palp, foot, mantle and adductor muscle in an order of decreasing pattern. Crude GAG was isolated from these tissues by cetyl pyridinium chloride (CPC) precipitation method and the obtained product was tested for its anticoagulant property along with standard heparin. The isolated GAG was also compared with standard heparin through FT-IR to find its relevant functional groups. The bivalve GAG was subjected to fractionation for further purification and its chemical components were analyzed. The subsequent comparison with the commercial heparin showed that K. opima could be a potent source of heparin for commercial use.]]>Achilleas, D.T.N., K. Karamanos and T. Tsegenidis,1999268388Andre, V.F.D.S.G., R. Onofre, D.M.P.O. Ednildo, A.M. Silvana, A. Luiz-Claudio and F. Silva,2006Periplaneta americana.]]>374146Bancroft, J.D. and H.C. Cook,1994Barlow, G.H., L.J. Coen and M.M. Mozen,196483272277Bitter, T. and H.M. Muir,19624330334Brimacombe, J.S. and J.M. Webber,19646181181Bullock, A.M., R.J. Roberts and L.D.M. Gordon,197656213226Burson, S.L., M.J. Fahrenbach, L.H. Frommhagen, B.A. Riccardi and R.A. Brown et al.,19567858745879Chatziioannidis, C.C., N.K. Karamanos, S.T. Anagnostides and T. Tsegenidis,199981187196Conrad, H.E.,1998Cowman, M.K., M.F. Slahetka, D.M. Hittner, J. Kim, M. Forino and G. Gadelrab,1984221707716Dietrich, P.C., J.F. de Paiva, C.T. Moraes, H.K. Takahashi, M.A. Porcionatto and H.B. Nader,1985Anomalocardia brasiliana.]]>84317Frommahagen, L.H., M.J. Fahrenbach, J.A. Vrockman and E.L.R. Stokstad,195382280283Gallagher, J.T.,1989112011218Grant, A.C., R.J. Linhardt, G.L. Fitzgerland, J.J. Park and R. Langer,19841372532Hardingham, T.E. and A.J. Fosang,19926861870Hovingh, P. and A. Linker,1982Homarus americanus).]]>25798409844Hovingh, P. and A. Linker,1993Anodonta californiensis, a freshwater mussel.]]>185263276Iozzo, R.V.,199867609652Jackson, E.L. and C.S. Hudson,19375920492050Jaques, L.B., L.W. Kavanagh and A. Lavallee,196717774778Jia, J., T. Toida, Y. Muneta, I. Kosiishi and T. Imanari et al.,2001Achatina fulica.]]>130513519Kavanagh, L.W. and L.B. Jaques,197323605611Koshiishi, I., E. Horikoshi and T. Imanari,1999267222226Kresse, H. and E. Schonnherr,2001189266278Lang, E.R.,19851985pp: 431-452pp: 431-452Lev, R. and S.S. Spicer,196412309309Lindahl, U., M. Gullberg-Kusche and L. Kjelen,19982732497924982Linhardt, R.J. and N.S. Gunay,199925516Lopes-Lima, M., I. Ribeiro, R.A. Pinto and J. Machado,2005Anodonta cygnea.]]>141319326Mollelo, J.A., G.P. Epling and R.W. Davis,196324573579Nader, H.B., S.F. Chavante, E.A. dos-Santos, F.W. Oliveira and J.F. de-Paiva et al.,199932529538Pejler, G., A. Danielson, I. Bjork, U. Lindahl, H.B. Nader and C.P. Dietrich,1987Anomalocardia brasiliana and Tivela mactroides.]]>2621141311421Pereira, M.S. and B. Mulloy,199927476567667Perrimon, N. and M. Bernfield,2000404725728Rice, K.G., Y.S. Kim, A.C. Grant and R.J. Linhardt,1985150325331Rice, K.G., M.K. Rottink and R.J. Linhardt,1987244515522Scott, H.R. and B.P. Clayton,19531336336Silva, L.C.F.,20022002pp: 1-14pp: 1-14Spicer, S.S.,196081835Subramanian, A., L. Kannan, T. Pugalendhi and A. Muruganantham,199773566566Toida, T., T. Maruyama, Y. Ogita, A. Suzuki, H. Toyoda, T. Imanari and R.J. Linhardt,199926233241Tsuji, A., T. Kinoshita, M. Hoshino and M. Takeda,19701825442547Turnbull, J., A. Powell and S. Guimond,2001117582Ulrich, N.P. and J.K. Boon,2001Mercenaria mercenaria.]]>78155159Farias, W.R.L., A.P. Valente, M.S. Pereire and P.A.S. Mourao,2000Botryocladia occidentalis and comparison of its anticoagulant action with that of sulfated galactans from invertebrates.]]>2752929929307Yeong, S.K., Y.Y. Jo, I. Chang and T.K. Toidas,1996Achatina fulica.]]>2711175011755