Trends in Bioinformatics1994-79412077-2254Asian Network for Scientific Information10.3923/tb.2018.17.24Gopal DasSasti Jyoti ChakrabortyHirak DattaAbhijit 12018111Background and Objective: Precursor microRNA expressions vary depending on their cellular environment and a large amount of genome segments can be folded in similar pseudo precursors microRNA hairpins like structure. Therefore, detection of true precursor microRNA in a genome is challenging task. The computational prediction of precursor MicroRNAs first distinguishes a large amount of similar folded hairpins like structure in genome sequence as a pseudo or true precursor miRNAs. However, researchers need to be improving methods for identification of precursor MicroRNA in a genomic sequence. Materials and Methods: In this computational method, supervised machine learning approach was used as a classifier for classifying the true precursor miRNAs using sequence and secondary structure information. Results: The support vector machine (SVM) classifier achieved accuracy (Q) of 96.28% for predicting true pre-miRNAs. Here, a new precursor miRNA identification tool-PremipreD was developed which performs better in comparison to existing tools, in terms of overall performance and specificity. Conclusion: The PremipreD algorithm reduces the number of false positive prediction rate by using effective Support vector machine methods.]]>Kim, V.N., J. Han and M.C. Siomi,200910126139Ruby, J.G., A. Stark, W.K. Johnston, M. Kellis, D.P. Bartel and E.C. Lai,2007Drosophila microRNAs.]]>1718501864Gulyaeva, L.F. and N.E. Kushlinskiy,20162016Ryu, S., N. Joshi, K. McDonnell, J. Woo and H. Choi et al.,20112011Melamed, Z.E., A. Levy, R. Ashwal-Fluss, G. Lev-Maor and K. Mekahel et al.,201350869881Friedlander, M.R., W. Chen, C. Adamidi, J. Maaskola, R. Einspanier, S. Knespel and N. Rajewsky,200826407415Stark, A., M.F. Lin, P. Kheradpour, J.S. Pedersen and L. Parts et al.,2007Drosophila genomes using evolutionary signatures.]]>450219232Gupta, H., T. Tiwari, M. Patel, A. Mehta and A. Ghosh,2015H. Annuus EST sequences.]]>6139144Wan, Y., M. Kertesz, R.C. Spitale, E. Segal and H.Y. Chang,201112641655Auyeung, V.C., I. Ulitsky, S.E. McGeary and D.P. Bartel,2013152844858Jinek, M. and J.A. Doudna,2009457405412Ding, J., S. Zhou and J. Guan,20102010Wu, Y., B. Wei, H. Liu, T. Li and S. Rayner,20112011Jiang, P., H. Wu, W. Wang, W. Ma, X. Sun and Z. Lu,200735W339W344Huang, K.Y., T.Y. Lee, Y.C. Teng and T.H. Chang,20152015Batuwita, R. and V. Palade,200925989995Huang, T.H., B. Fan, M.F. Rothschild, Z.L. Hu, K. Li and S.H. Zhao,20072007Liu, B., L. Fang, F. Liu, X. Wang, J. Chen and K.C. Chou,20152015Liu, B., L. Fang, F. Liu, X. Wang and K.C. Chou,201634223235Liu, B., L. Fang, S. Wang, X. Wang, H. Li and K.C. Chou,2015385153159Liu, B., L. Fang, J. Chen, F. Liu and X. Wang,20151111941204Xue, C., F. Li, T. He, G.P. Liu, Y. Li and X. Zhang,20052005Kozomara, A. and S. Griffiths-Jones,201139D152D157Wei, L., M. Liao, Y. Gao, R. Ji, Z. He and Q. Zou,201411192201Chan, P.P. and T.M. Lowe,200837D93D97Tempel, S., B. Zerath, F. Zehraoui and F. Tahi,201521775785Lorenz, R., S.H. Bernhart, C.H. Zu Siederdissen, H. Tafer, C. Flamm, P.F. Stadler and I.L. Hofacker,20162016Jorquera, R., R. Ortiz, F. Ossandon, J.P. Cardenas, R. Sepulveda, C. Gonzalez and D.S. Holmes,20162016