Trends in Bioinformatics1994-79412077-2254Asian Network for Scientific Information10.3923/tb.2013.45.61In silico Structural Analysis of 16S rDNA Sequences of Bacteria Isolated from Keratitis Patients]]>BhaskarAnusha NithyaV. 2201362Keratitis is a condition in which the cornea of the eye becomes inflamed. It is caused by bacteria, viruses, fungi and parasites. The diagnosis of the causative organism of keratitis remains a problem due to lack of advanced techniques. In this study PCR and sequence analysis of 16S rDNA was used to ascertain the bacteria isolated in eye swabs of keratitis patients. The bacterial genes were sequenced and deposited in the GenBank (NCBI) with the accession numbers JN378393, JN378392, HM204502, JQ039348, JN652127, JN652129, JQ039350, JN652128, JQ039349 and HQ404365 for Streptococcus viridan, Staphylococcus aureus, Micrococcus sp., Moraxella sp., Citrobacter koseri, Acinetobacter baumannii, Pseudomonas aeruginosa, Klebsiella sp., Propionibacterium sp. and Staphylococcus epidermidis, respectively. Sequences were analyzed and aligned by ClustalX and a phylogenetic relationship was studied. The secondary structure of the 16S DNA was developed using GeneBee which produces secondary structures prediction through the minimization of the potential energy of the system. These results may be useful in characterizing the micro evolutionary mechanisms of the species for researchers.]]>Whitcher, J.P. and M. Srinivasan,199781622623Dunlop, A.A., E.D. Wright, S.A. Howlader, I. Nazrul, R. Husain, K. McClellan and F.A. Billson,199422105110Upadhyay, M.P., P.C. Karmacharya, S. Koirala, N.R. Tuladhar, L.E. Bryan, G. Smolin and J.P. Whitcher,19911119299Wilhelmus, K.R.,19961996pp: 970-1031pp: 970-1031Asbell, P. and S. Stenson,19821007780McDonnell, P.J. and W.R. Green,19901990pp: 981-987pp: 981-987Jones, D.B.,198179153167Laibson, P.R.,197288553574Liesegang, T.J. and R.K. Forster,1980903847Baum, J.L.,19792497105Morlet, N. and M. Daniell,20038711691172Jones, D.B.,197924105116Baum, J.L.,19782992831Hyndiuk, R.A.,1981Pseudomonas keratitis.]]>79541624Bharathi, M.J., R. Ramakrishnan, S. Vasu, Meenakshi and R. Palaniappan,2002201924Thompson, J.D., T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins,19972548764882Mathews, D.H., J. Sabina, M. Zuker and D.H. Turner,1999288911940Zuker, M. and P. Stiegler,19819133148Niyyati, M., S. Rezaie, F. Rahimi, M. Mohebali, A.H. Maghsood, S.H. Farnia and M. Rezaeian,2008Acanthamoeba castellanii as a major agent of Acanthamoeba keratitis.]]>37914