Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2016.45.57NgengeTamfu Alfred CarolDomgnim Mokam Elisabeth EmmanuelTalla VernyuyTan Paul JosephMbafor Tanyi PopovaMilena BankovaVassya 22016102Three extracts of propolis harvested from Nkambe, North-West region of Cameroon were characterized by GC-MS analysis and their gastric cytoprotective, antisecretory and antioxidant properties evaluated using experimentally-induced gastric ulcers in rats. The propolis extracts were rich in phytoconstituents occurring as sugars, triterpenes and a mixture (fatty acids+triterpenes+alkenyl resorcinols) in the methanol, hexane and acetone extracts, respectively with Mangifera indica as major plant source. Three triterpenes, lupeol, lupenone, 27-hydroxymangiferonic acid and an ester of fatty acid heptadecyl butanoate were isolated and characterized. The methanol, acetone and hexane extracts (200-600 mg kg1) dose-dependently prevented the formation of ethanol-induced gastric lesions (percentage of inhibition, 61, 54 and 55%, respectively, at the dose of 600 mg kg1). Increasing doses of the extracts inhibited pylorus ligation-induced lesions by 64.5, 73.1 and 16.2%, respectively for the highest dose but none of them showed antisecretory activity compared with controls. The most further significantly (p<0.01) reduced HCl/ethanol-induced ulcer indices from 4.33+0.32 in cytoprotective (acetone) extract (56.6-73.1% inhibition under highly acidic gastric environments), the controls to 1.25+0.53 and 0.6+0.04 at the dose of 400 and 600 mg kg1, respectively (percentage of inhibition: 71-86%). Furthermore, upon pretreatment of the rats with indomethacin prior to HCl/ethanol, the acetone extract significantly (p<0.001) decreased ulcer index from 5.55+0.73 in the controls to 1.89+0.15 at the dose of 600 mg kg1. Although pretreatment with indomethacin reduced the protective effect of the acetone extract by 23-27% and cytoprotection remained high (62-66% inhibition). The cytoprotective action of the most active (acetone) extract may involve the mediation of endogenous prostaglandins.]]>Del Valle, J.,20052005pp: 1746-1762pp: 1746-1762Yuan, Y., I.T. Padol and R.H. Hunt,200638089Wallace, J.L. and D.N. Granger,199610731740Lima, Z.P., J.A. Severi, C.H. Pellizzon, A.R.M.S. Brito and P.N. Solis et al.,20061062937Bandyopadhyay, D., K. Biswas, M. Bhattacharyya, R.J. Reiter and R.K. Banerjee,20011501513Kaunitz, J.D. and Y. Akiba,200420526532Tarnawski, A.S.,200550S24S33Wolfe, M.M. and G. Sachs,2000118S9S31Dharmani, P., P.K. Mishra, R. Maurya, V.S. Chauhan and G. Palit,2005Allophylus serratus: A plant with potential anti-ulcerogenic activity.]]>99361366Sharma, R.K., S. Mandal, G.P. Rajani, N. Gupta and D.P. Srivastava,2011Polyalthia longifolia in rats.]]>3351359Srinivas, T.L., S.M. Lakshmi, S.N. Shama, G.K. Reddy and K.R. Prasanna,201329197Gilani, A.H. and Atta-ur-Rahman,20051004349Sanpa, S., M. Popova, V. Bankova, T. Tunkasiri, S. Eitssayeam and P. Chantawannakul,2015Tetragonula laeviceps and Tetrigona melanoleuca (Hymenoptera: Apidae) from Thailand.]]>2015Talla, E., B. Dabole, G.S. Taiwe, T. Ngo Bum and J.T. Mbafor et al.,20134218227Mbawala, A., D. Roger, F.N.T. Fohouo and J.B. Milliere,2010In vitro susceptibility of some bacteria strains to ethanol extracts of propolis collected from two different geographic origins in Cameroon.]]>8217222Paul, S., T. Emmanuel, C. Matchawe, T.T. Alembert and Z.M. Elisabet et al.,2014419Lotfy, M.,200672231Nolkemper, S., J. Reichling, K.H. Sensch and P. Schnitzler,201017132138Paulino, N., A.P. Dantas, V. Bankova, D.T. Longhi, A. Scremin, S.L. de Castro and J.B. Calixto,2003in vitro contraction of airway smooth muscle.]]>93307313Bankova, V.,200522932Bankova, V.,2005100114117Awale, S., F. Li, H. Onozuka, H. Esumi, Y. Tezuka and S. Kadota,200816181189Kujumgiev, A., I. Tsvetkova, Y. Serkedjieva, V. Bankova, R. Christov and S. Popov,199964235240Orsolic, N.,2010in vivo and in vitro.]]>2120Ahn, M.R., S. Kumazawa, Y. Usui, J. Nakamura, M. Matsuka, F. Zhu and T. Nakayama,200710113831392Njintang, Y.N., N.L. Tatsadjieu, A. Ngakou, D. Danra and F.N. Tchuenguem-Fohouo,2012Propolis.]]>25663Banskota, A.H., Y. Tezuka, I.K. Adnyana, K. Midorikawa and K. Matsushige et al.,200072239246Farooqui, T. and A.A. Farooqui,20106186199De Barros, M.P., M. Lemos, E.L. Maistro, M.F. Leite, J.P.B. Sousa, J.K. Bastos and S.F. de Andrade,2008120372377Segueni, N., A.A. Magid, M. Decarme, S. Rhouati and M. Lahouel et al.,2011779991004Abo-Salem, O.M., R.H. El-Edel, G.E. Harisa, N. El-Halawany and M.M. Ghonaim,200922205210Sforcin, J.M. and J.M. Bankova,2011133253260Sforcin, J.M.,2007113114Bankova, V.S., S.L. de Castro and M.C. Marcucci,200031315Hara, N. and S. Okabe,198585443446Tan, P.V., N.G. Nditafon, M.P. Yewah, T. Dimo and F.J. Ayafor,1996Eremomastax speciosa: Effects of leaf aqueous extract on ulcer formation and gastric secretion in rats.]]>54139142Shay, H., S.A. Komarow, S.S. Fels, D. Meranze, M. Gruenstein and H. Siplet,194554361Ellman, G.L.,1959827077Wilbur, K.M., F. Bernheim and O.W. Shapiro,194924305310Gomez-Caravaca, A.M., M. Gomez-Romero, D. Arraez-Roman, A. Segura-Carretero and A. Fernandez-Gutierrez,20064112201234Savka, M.A., L. Dailey, M. Popova, R. Mihaylova and B. Merritt et al.,20152015Rushdi, A.I., N. Adgaba, N.I. Bayaqoob, A. Al-Khazim, B.R. Simoneit, A.H. El-Mubarak and K.F. Al-Mutlaq,20142014Papachroni, D., K. Graikou, I. Kosalec, H. Damianakos, V. Ingram and I. Chinou,2015106770Silva-Carvalho, R., F. Baltazar and C. Almeida-Aguiar,20152015Kardar, M.N., T. Zhang, G.D. Coxon, D.G. Watson, J. Fearnley and V. Seidel,2014106156163Almutairi, S., B. Eapen, S.M. Chundi, A. Akhalil and W. Siheri et al.,2014103539Zhang, T., R. Omar, W. Siheri, S. Al Mutairi and C. Clements et al .,2014120181190De Castro Ishida, V.F., G. Negri, A. Salatino and M.F.C.L. Bandeira,2011125966972Abd El Hady, F.K. and A.G. Hegazi,200257386394Hegazi, A.G. and F.K. Abd El Hady,200257395402Hernandez-Vazquez, L., S. Mangas, J. Palazon and A. Navarro-Ocana,201031476480Kartal, M., S. Kaya and S. Kurucu,200257905909Baykara, M., S. Silici, M. Ozcelik, O. Guler, N. Erdogan and M. Bilgen,2015In vivo nephroprotective efficacy of propolis against contrast-induced nephropathy.]]>21317321Popova, M., R. Dimitrova, H.T. Al-Lawati, I. Tsvetkova, H. Najdenski and V. Bankova,20132013Araujo, M.J.A.M., M.C. Bufalo, J.C. Bruno, A. Fernandes Jr., B. Trusheva, V. Bankova and J.M. Sforcin,2015Melipona fasciculata Smith in Northeast Brazil.]]>41220Trusheva, B., M. Popova, E.B. Koendhori, I. Tsvetkova, C. Naydenski and V. Bankova,201125606613Dongock, D.N., J. Foko, J.Y. Pinta, L.V. Ngouo, J. Tchoumboue and P. Zango,200422139145Focho, D.A., M.C. Newu, M.G. Anjah, F.A. Nwana and F.B. Ambo,20092009Silva, M.S.S., S.G. de Lima, E.H. Oliveira, J.A.D. Lopes, M.H. Chaves, F.A.M. Reis and A.M.G.L. Cito,2008335358Teerasripreecha, D., P. Phuwapraisirisan, S. Puthong, K. Kimura and M. Okuyama et al.,2012In vitro antiproliferative/cytotoxic activity on cancer cell lines of a cardanol and a cardol enriched from Thai Apis mellifera propolis.]]>2012Christov, R., B. Trusheva, M. Popova, V. Bankova and M. Bertrand,200620531536Kurek-Gorecka, A., A. Rzepecka-Stojko, M. Gorecki, J. Stojko, M. Sosada and G. Swierczek-Zieba,20141978101Li, F., S. Awale, Y. Tezuka and S. Kadota,20081654345440Oates, P.J. and J.P. Hakkinen,1988941021Srikanth, J. and P. Muralidharan,2009Morinda citrifolia Linn fruit extract.]]>1345352Tulassay, Z. and L. Herszenyi,20102499108Amang, P.A., P.V. Tan, S.A. Patamaken and M.N. Mefe,2014Eremomastax speciosa in rats.]]>11165171Perfusion, A.A., P.V. Tan, N. Ernestine and N. Barthelemy,2014Eremomastax speciosa (Acanthaceae) occurs through antihistaminic and anticholinergic pathways.]]>2014Gharzouli, K., A. Gharzouli, S. Amira and S. Khennouf,200153175180Gharzouli, K., S. Amira, A. Gharzouli and S. Khennouf,200254217221Gharzouli, K., A. Gharzouli, S. Amira and S. Khennouf,199939151156Lee, B.I., A. Nugroho, M.S. Bachri, J. Choi and K.R. Lee et al.,2010Ligularia stenocephala.]]>33493497Lewis, D.A. and P.J. Hanson,199128201231Gupta, M.B., R. Nath, G.P. Gupta and K.P. Bhargava,198173649652Favier, A.,2003269-270108115Morikawa, T., N. Li, A. Nagatomo, H. Matsuda, X. Li and M. Yoshikawa,2006Camellia sinensis).]]>69185190Sun, H., W.S. Fang, W.Z. Wang and C. Hu,200647339368Rice-Evans, C.A., N.J. Miller and G. Paganga,199620933956Akinmoladun, A.C., E.O. Ibukun, E. Afor, B.L. Akinrinlola and T.R. Onibon et al.,2007Alstonia boonei.]]>611971201Whittle, B.J.,197760455460Flemstrom, G., A. Garner, O. Nylander, B.C. Hurst and J.R. Heylings,19823- transport by mammalian duodenum in vivo.]]>243G348G358Miller, T.A.,1983245G601G623Selling, J.A., D.L. Hogan, A. Aly, M.A. Koss and J.I. Isenberg,1987106368371Robert, A.,19811981pp: 1407-1437pp: 1407-1437Konturek, S.J., I. Piastucki, T. Brzozowski, T. Radecki, A. Dembinska-Kiec, A. Zmuda and R. Gryglewski,19818049Robert, A., J.E. Nezamis, C. Lancaster, J.P. Daris, S.O. Field and A.J. Hanchar,1983245G113G121Yamamoto, K., H. Kakegawa, H. Ueda, H. Matsumoto, T. Sudo, T. Miki and T. Satoh,199258389393