Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2015.41.55Juniperus Against H2O2 Induced Oxidative Damage in Human Erythrocytes and Leucocytes]]>BaisSouravh PrasharYash 2201592The aim of the present study was to investigate the protective effects of amentoflavone (AF), isolated from six species of Juniperus (AF1-AF6) (Cupressaceae) Juniperus communis L. (AF1), Juniperus wallichiana L. (AF2), Juniperus indica L. (AF3), Juniperus macropoda L. (AF4), juniperus recurva L. (AF5) and Juniperus polycarpos L. (AF6)] against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The experiment was set and designed as per method available in literature. Amentoflavone was isolated from aerial parts of Juniperus species (above six) and authenticated by TLC, IR, HPLC and physicochemical parameters. The two concentration of isolated AF (A = 250 μL, B = 500 μL) were used to assessed its protective effects against H2O2 induced oxidative damage in human erythrocytes and leucocytes. The measurements of different antioxidant parameters like, CAT, SOD and GPx, LPO and GSH levels were performed under each set extracts. Juniperus communis L. (AF1B) (500 μL) and Juniperus indica L. (AF3B) (500 μL) were the most significant one on CAT (28.35±2.980 and 27.73±1.580 AU g1 Hb), GPx (128.53±3.10 and 41.7±1.420 μg g1 Hb) and SOD (1193.5±19.7 and 1532.6±17.60 U g1 Hb) enzyme systems of erythrocytes. Among plant extracts, Juniperus communis L. (AF1B) showed the highest activities on CAT (13.79±1.832 AU mg1 protein) and SOD (820±14.50 U mg1 protein) while Juniperus indica L. (AF3B) showed significant effects on Gpx (152.72±3.70 U mg1 protein), GSH (25.93±1.560 μg mg1 protein) and LPO (10.54±2.90 nmol mg1 protein) enzyme systems of leucocytes. 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