Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2014.172.187Ficus luschnathiana
and its Application in the Generation of Whey Antimicrobial Peptides]]>BarrosM. CanteraA.M.B. 4201484Characterization studies were performed to a proteolytic extract, prepared
from latex of Ficus luschnathiana (Miq.) Miq., a Moraceae family tree.
The mechanism of action was determined, corresponding to serine proteinases
behaviour. Studies of optimum pH and temperature showed a maximum catalytic
activity at pH 8.0 and 75°C, respectively. The proteolytic activity exhibited
good stability when incubated at different pH and temperature conditions. At
7°C and pH 7.5, the extract kept a 100% of the initial activity for 5 months.
When comparing the effect of pH on the stability, maximum values were obtained
at pH 6.5, with 98% of the initial activity remaining after 5 h of incubation
at 60°C. After 1 min at 100°C total inactivation occurred. Regarding
the potential use in the dairy industry, milk clotting activity was determined
obtaining a very effective coagulant action. Analysis protocols which included
molecular exclusion chromatography, electrophoretic methods and mass spectrometry
revealed a complex protein profile. It was found that the molecules responsible
for the proteolytic activity of the extract were several proteins of different
molecular weight and isoelectric points, most of which still retained the activity
in presence of SDS and reducing agents. These proteic components displayed a
pH-dependant tendency to form aggregates. Using the proteolytic activity of
the crude extract under controlled hydrolysis conditions on whey proteins, yielded
peptidic fractions with antimicrobial activity against a S. aureus strain.
It was found that fractions of peptide concentration of 0.96 μmol mL-1
inhibited growth by 94%.]]>Andrews, B.A. and J.A. Asenjo,1986Cytophaga sp. In batch and continuous culture.]]>2813661375Azarkan, M., A. Matagne, R. Wattiez, L. Bolle, J. Vandenameele and D. Baeyens-Volant,2011Ficus carica latex.]]>7217181731Brunius, G. and B.L. Sundbom,19872+ on the thermal stability of trypsin in phosphate-buffered saline solution used for harvesting of human embryonic lung fibroblast cultures.]]>15265270Castro, S. and A.M.B. Cantera,1995234143Church, F.C., H.E. Swaisgood, D.H. Porter and G.L. Catignani,1983o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins.]]>6612191227Clare, D.A., G.L. Catignani and H.E. Swaisgood,2003912391255Devaraj, K.B., L.R. Gowda and V. Prakash,2008Ficus racemosa (L.).]]>69647655Hadacek, F. and H. Greger,200011137147Kumari, M., A. Sharma and M.V. Jagannadham,2012Ficus religiosa.]]>13112951303Layne, E.,19571957pp: 447-454pp: 447-454Liggieri, C., C.L. Natalucci, W.D. Obregon, C. Sequeiros, S. Trejo and N. Priolo,2009Asclepiadaceae.]]>2009pp: 13-23pp: 13-23Obregon, W.D., C.S. Liggieri, S.A. Trejo, F.X. Aviles, S.E. Vairo-Cavalli and N.S. Priolo,2009Asclepias curassavica by molecular biology validated by proteomic approach.]]>9114571464Pellegrini, A.,2003912251238Pereira, L.S., V.M. Gomes, K.V.S. Fernandes, M.P. Sales and J. Xavier-Filho,1999Manihot glaziovii Muell. Arg.]]>222730Recio, I. and S. Visser,200010597605Salvesen, G.S. and H. Nagase,20012001pp: 105-130pp: 105-130Sharma, A., M. Kumari and M.V. Jagannadham,2012Ficus religiosa.]]>47914921Singh, D., B. Singh and R.K. Goel,2011Ficus religiosa: A review.]]>134565583Singh, V.K., A.K. Patel, A.J. Moir and M.V. Jagannadham,2008Morus indica cv. K2.]]>6921102119Spadaro, A.C.C., W. Draghetta, S.N. del Lama, A. Camargo and L.J. Greene,197996317321