Research Journal of Phytochemistry1819-34712151-6081Science International10.3923/rjphyto.2010.78.86Camellia sinensis)]]>ChakrabortyDevjani ChakrabortiSunanda 2201042The antibacterial activity of methanolic extract of green tea leaves (Camellia sinensis; Family: Theaceae) was studied on four different bacteria namely Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus and two fungi of Aspergillus species. Antibacterial activity was assigned by measuring the Zone of Inhibition (ZOI) as well as Minimum Inhibitory Concentration (MIC) at four different concentrations of the methanolic extract (10, 25, 50 and 100 mg mL-1) and erythromycin (10 mg mL-1) which was taken as the antibiotic control. Preliminary screening was done to find out the phytochemical components present in the extract. Thin Layer Chromatography (TLC) was performed to separate and isolate the bioactive components present in the extract. Further bioassay-guided fractionation of the methanolic extract with the help of column chromatography was performed using a stepwise gradient with Chloroform: Methanol against the bacteria. The fraction which gave us the best result was further taken for the HPTLC for the identification of the bioactive compound which was identified as catechin. Zone of inhibition was observed in most of the organism. Alcoholic extract of the leaves of Camellia sinensis was found to be most effective against Bacillus cereus.]]>Ahn, Y., S. Sakanaka and J. Kim,19903335338Daniel, M.,19911st Edn.,Hara, Y. and T. Ishigami,198936996999Harborne, J.B.,19732nd Edn.,pp: 85pp: 85Hironori, T.,1999593444Akiyama, H., K. Fujii, O. Yamasaki, T. Oono and K. Iwatsuki,2001Staphylococcus aureus.]]>48487491Horiba, N., Y. Makewa, M. Ito and T. Yamamoto,199117122124Marderosian, D.A.,1999John, T. and P. Mukundan,197969542545Chung, K.T., T.Y. Wong, C.I. Wei, Y.W. Huang and Y. Lin,199838421464Kuroda, Y. and Y. Hara,19994366997Mabata, T., I. Lu and A.D. Saikia,2006Camellia sinensis) on Listeria monocytogenes.]]>2234238Nakane, H. and K. Ono,19892928412845Nance, C.L., M. Williamson, T. Cormick, S. Paulson and S. Shearer,200624119124NCCLS.,2002Okpuzor, J., H. Ogbunugafor, G. Kareem and M.I. Ezikpe,2009In vitro investigation of antioxidant phenolic compound extracts of Senna alata.]]>36876Shetty, M., K. Subbannayya and P.G. Shivananda,1994Camellia sinensis and coffee Coffee arabica with special reference to Salmonella typhimurium.]]>26147150Stapleton, P.D., S. Shah, K. Ehlert, Y. Hara and P.W. Taylor,2007Staphylococcus aureus.]]>15320932103Sumpio, B.E., A.C. Cordova, D.W.B. Schlessel, F. Qin and Q.H. Chen,2006202813825Toda, M., S. Okubo, R. Ohnishi and T. Shimamura,198944669672Vinson J.A. and Y.A. Dabbagh,19984334446WHO Protocol CG-06,19831983Jane, H.,20012001Toda, M., S. Okubo, R. Hiyoshi and T. Shimamura,19898123125