Research Journal of Nanoscience and Nanotechnology 1996-5044 xxxx-xxxx Asian Network for Scientific Information 10.3923/rjnn.2013.1.18 K.Chandrasekharan Dhanya Krishnan Krishnan Nair Cherupally 1 2013 3 1 Silver nanoparticles were prepared from silver nitrate using Glycyrrhizic Acid (GLY), under sonication. The resultant golden yellow solution containing glycyrrhizic acid-silver nanoparticle complex (GLY-Ag) of particle size of less than 100 nm was analyzed for antioxidant activity by DPPH free radical scavenging activity and it was shown that GLY-Ag possesses higher free radical scavenging activity than GLY. Oral administration of GLY-Ag offered significant protection against gamma radiation induced cellular DNA damage as shown by the results of comet assay performed in bone marrow cells and blood leukocytes of mice exposed to various doses of whole body gamma radiation (0-6 Gy). GLY-Ag was also found to offer protection against the genotoxic effects of ionising radiation as shown by the results of micronucleus assay and chromosomal aberration analysis in whole body gamma irradiated mice (0-2 Gy). The complex offered significant protection to the hemopoeitic system from radiation injury (6 Gy) as shown by bone marrow cellularity, total blood count and endogenous spleen colony formation. Oral administration of GLY-Ag enhanced the survival of animals following a lethal dose of whole body gamma irradiation (8 Gy).

]]> Schmidt-Ullrich, R.K., P. Dent, S. Grant, R.B. Mikkelsen and K. Valerie, 2000 Radiat. Res., 153 245 257 Mettler, Jr. F.A. and G.L. Voelz, 2002 N. Engl. J. Med., 346 1554 1561 Maurya, D.K., T.P. Devasagayam and C.K. Nair, 2006 Indian J. Exp. Biol., 44 93 114 Nair, C.K.K., D.K. Parida and T. Nomura, 2001 J. Radiat. Res., 159 812 834 Coleman, C.N., H.B. Stone, J.E. Moulder and T.C. Pellmar, 2004 Science, 304 693 694 Fujisawa, Y., M. Sakamoto, M. Matsushita, T. Fujita and K. Nishioka, 2000 Microbiol. Immunol., 44 799 804 Dehpour, A.R., M.E. Zolfaghari, T. Samadian, F. Kobarfard, M. Faizi and M. Assari, 1995 Int. J. Pharm., 119 133 138 Ito, A., N. Hayashi, K. Katayama, H. Hagiwara and A. Kasahara et al., 1997 Int. Hepatol. Commun., 50 233 238 Cinatl, J., B. Morgenstern, G. Bauera, P. Chandra, H. Rabenau and H.W. Doerr, 2003 Lancet, 361 2045 2046 Gandhi, N.M., D.K. Maurya, V. Salvi, S. Kapoor, T. Mukherjee and C.K. Nair, 2004 J. Radiat. Res., 45 461 468 Lin, H., D.M. Hau, W.C. Chen, K.T. Chen and J.G. Lin, 1996 Chinese Med. J., 109 138 142 Bhatia, A.L., 2008 Asian J. Exp. Sci., 22 33 44 Atiyeh, B.S., M. Costagliola, S.N. Hayek and S.A. Dibo, 2007 Burns, 33 139 148 Khaydarov, R.A., R.R. Khaydarov, O. Gapurova, Y. Estrin and T. Scheper, 2009 J. Nanopart. Res., 11 1193 1200 Khanna, P.K. and C.K.K. Nair, 2009 Int. J. Green Nanotechnol. Phy. Chem., 1 P3 P9 Von Gadow, A., E. Joubert and C.T. Hannsman, 1997 Aspalanthus linearis) α-tocoferol BHT and BHA.]]> J. Agric. Food Chem., 45 632 638 Singh, N.P. and R.E. Stephens, 1997 Mutat. Res./DNA Repair, 383 167 175 Chandrasekharan, D.K., T.V. Kagiya and C.K. Nair, 2009 in vitro, ex vivo, in vivo and oxidative stress in vivo.]]> J. Radiat. Res., 50 203 212 Konca, K., A. Lankoff, A. Banasik, H. Lisowka and T. Kuszewski et al., 2003 Mutat. Res. /Genet. Toxicol. Environ. Mutagenesis, 534 15 20 Hayashi, M., T. Morita, Y. Kodama, T. Sofuni and M. Ishidate, 1990 Mutat. Res., 245 245 249 Devi, P.U., K.S. Bisht and M. Vinita, 1998 Br. J. Radiol., 71 782 784 Till, J.E. and E.A.M. Culloch, 1961 Radiat. Res., 14 213 222 Draganic, I.G. and Z.D. Draganic, 1971 The Radiation Chemistry of Water. Pages: 242 Pages: 242 Widel, M., S. Jedrus, B. Lukaszczyk, K. Raczek-Zwierzycka and A. Swierniak, 2003 Radiat. Res., 159 713 721 Chen, Y.M., S.L. Lin, W.C. Chiang, K.D. Wu and T.J. Tsai, 2006 Kidney Int., 69 1410 1415 Floersheim, G.L., N. Chiodetti and A. Bieri, 1988 Br. J. Radiol., 61 501 508 Coogle, J.E., 1983 Biological Effects of Radiation. 2nd Edn., Pages: 247 Pages: 247 Schubert, D., R. Dargusch, J. Raitano and S.W. Chan, 2006 Biochem. Biophys. Res. Commun., 342 86 91 Tarnuzzer, R.W., J. Colon, S. Patil and S. Seal, 2005 Nano Lett., 5 2573 2577 Das, M., S. Patil, N. Bhargava, J.F. Kang, J.M. Riedel, S. Seal and J.J. Hickman, 2007 Biomaterials, 28 1918 1925 Rzigalinski, B.A., D. Bailey, L. Chow, S.C. Kuiry, S. Patil, S. Merchant and S. Seal, 2003 FASEB, 17 A606 A606 Ali, S.S., J.I. Hardt, K.L. Quick, J.S. Kim-Han and B.F. Erlanger et al., 2004 Free Radical Bio. Med., 37 1191 1202 Daroczi, B., G. Kari, M.F. McAleer, J.C. Wolf, U. Rodeck and A.P. Dicker, 2006 In vivo radioprotection by the fullerene nanoparticle df-1as assessed in a zebrafish model.]]> Clin. Cancer Res., 12 7086 7091 Trajkovic, S., S. Dobric, A. Djordjevic, V. Dragojevic-Simic and Z. Milovanovic, 2005 Mater. Sci. Forum, 494 549 554 Trajkovic, S., S. Dobric, V. Jacevic, V. Dragojevic-Simic, Z. Milovanovic and A. Djordjevic, 2007 Colloids Surf. B: Biointerfaces, 58 39 43 Mirkov, S.M., A.N. Djordjevic, N.L. Andric, S.A. Andric, T.S. Kostic et al., 2004 60(OH)24.]]> Nitric Oxide, 11 201 207 Kataoka, Y., J.S. Murleya, K.L. Bakera and D.J. Grdinaa, 2007 Radiat. Res., 168 106 114 Mahrhofer, H., S. Burger, U. Oppitz, M. Flentje and C.S. Djuzenova, 2006 Int. J. Radiat. Oncol., 64 573 580 Divyasree, S. and C.K.K. Nair, 2010 Acorus calamus Linn.: An in vitro study.]]> Environ. Toxicol. Pharmacol., 29 302 307 Mendiola-Cruz, M.T. and P. Morales-Ramirez, 1999 in vivo.]]> Mutat. Res., 433 45 55 Till, J.E. and E.A.M. Culloch, 1963 in vivo.]]> Radiat. Res., 18 96 105 Injac, R., N. Radic, B. Govedarica, A. Djordjevic and B. Strukelj, 2008 Afr. J. Biotechnol., 7 4940 4950 Rzigalinski, B.A., K. Meehan, R.M. Davis, Y. Xu, W.C. Miles and C.A. Cohen, 2006 Nanomedicine, 1 399 412 Chandrasekharan, D.K. and C.K.K. Nair, 2010 Int. J. Low Radiat., 7 453 466 Chandrasekharan, D.K., P.K. Khanna and C.K.K. Nair, 2011 Mutat. Res. Gen. Toxicol. Environ. Mutagen., 723 51 57 Khanna, P.K., N. Singh, D. Kulkarni, S. Deshmukh, S. Charan and P.V. Adhyapak, 2007 Mat. Lett., 61 3366 3370