Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2017.55.61Persea americana Mill.) Peel in vitro]]>AntasionastiIrma RiyantoSugeng RohmanAbdul 22017112Background: Synthetic-based antioxidants are suspected to cause toxic or mutagenic effects toward human health, therefore,
the demand for natural antioxidants increased. One of the natural antioxidant sources is Avocado Peel (AP). Objective: The
objective of this study is to determine antioxidant activities of the extract, fractions and isolates from AP using in vitro method
of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical
scavenging, reducing power of iron (III) as well as total phenolic and flavonoid contents. Materials and Methods: Ethyl acetate fraction
of AP is fractionated using vacuum liquid chromatography and gravitation column chromatography methods. To identify the chemical
structure of the active compound of antioxidants in AP, FTIR spectroscopy and gas chromatography-mass spectrometry (GC-MS) are used.
Results: Among extracts evaluated, methanol extract has the strongest antioxidant activity. Further fractionation of methanolic extract
showed that fraction 8 is the most active fraction with IC50 values of 4.221±0.137 mg mL1 (DPPH assay) and 0.855±0.013 mg mL1
(ABTS assay), with reducing power of iron (III) of 723.067±18.849 mg ascorbic acid g1 fraction. Phenolics contents contributed
37, 40.78 and 47.45%, compared with the contribution of flavonoids compounds of 26.71, 19.25 and 34.62% for DPPH radical scavenging,
ABTS radical scavenging and reducing power of iron (III) to iron (II), respectively. Isolation of the fraction 8 of methanol extract
indicated the presence of 1,2,4-trihidroksiheptadek-12,16-diyne and isolation of the ethyl acetate extract indicated the presence of
1,2,4-trihidroksiheptadek-16-yne-18-ene. Conclusion: The AP is potential to be used as food supplement due to its antioxidant activities.]]>Carocho, M. and I.C.F.R. Ferreira,2013511525Aktumsek, A., G. Zengin, G.O. Guler, Y.S. Cakmak and A. Duran,2013Centaurea L. species.]]>55290296Young, I.S. and J.V. Woodside,200154176186Arina, N.B. and A. Rohman,2013Phyllantus urinaria L.]]>2011191124Di Carlo, G., N. Mascolo, A.A. Izzo and F. Capasso,199965337353Alnajar, Z.A.A., M.A. Abdulla, H.M. Ali, M.A. Alshawsh and A.H.A. Hadi,2012Melastoma malabathricum.]]>1735473559Valentao, P., E. Fernandes, F. Carvalho, P.B. Andrade, R.M. Seabra and M.L. Bastos,2002Cynara cardunculus L.) infusion against superoxide radical, hydroxyl radical and hypochlorous acid.]]>5049894993Tachakittirungrod, S., S. Okonogi and S. Chowwanapoonpohn,2007103381388He, N., Z. Wang, C. Yang, Y. Lu and D. Sun et al.,2009Longan pericarp.]]>70219224Rohman, A., S. Riyanto, Mistriyani, Shuhaira and A.E. Nugroho,2017114247Permatasari, L. and A. Rohman,2016Nephelium lappaceum L.) peel.]]>107580Mfonobong, A., U. Ismail, I.H. Mairo, Z. Maimuna and D. Fatima,2013Persea americana Mill seeds in Wistar rats fed a high-fat high-cholesterol diet.]]>3110Saavedra, J., A. Cordova, R. Navarro, P. Diaz-Calderon and C. Fuentealba et al.,20171988190Ayala-Zavala, J.F., V. Vega-Vega, C. Rosas-Dominguez, H. Palafox-Carlos and J.A. Villa-Rodriguez et al.,20114418661874Dabas, D., R.M. Shegog, G.R. Ziegler and J.D. Lambert,2013Persea Americana) seed as a source of bioactive phytochemicals.]]>1961336140Calderon-Oliver, M., H.B. Escalona-Buendia, O.N. Medina-Campos, J. Pedraza-Chaverri, R. Pedroza-Islas and E. Ponce-Alquicira,2016654652Daiuto, E.R., M.A. Tremocoldi, S.M. de Alencar, R.L. Vieites and P.H. Minarelli,201436417424Kikuzaki, H., M. Hisamoto, K. Hirose, K. Akiyama and H. Taniguchi,20025021612168Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans,19992612311237Hinneburg, I., H.J.D. Dorman and R. Hiltunen,200697122129Chun, O.K., D.O. Kim and C.Y. Lee,20035180678072Zou, Y., Y. Lu and D. Wei,2004Hypericum perforatum L. in vitro.]]>5250325039Kosinska, A., M. Karamac, I. Estrella, T. Hernandez, B. Bartolome and G.A. Dykes,2012Persea americana Mill. peels and seeds of two varieties.]]>6046134619Floegel, A., D.O. Kim, S.J. Chung, S.I. Koo and O.K. Chun,20112410431048Biskup, I., I. Golonka, A. Gamian and Z. Sroka,201367958963, (In Polish)Silverstein, R.M., F.X. Webster and D.J. Kiemle,20057th Edn.,Pages: 502Pages: 502Adikaram, N.K.B., D.F. Ewing, A.M. Karunaratne and E.M.K. Wijeratne,1992319396Rodriguez-Sanchez, D.G., A. Pacheco, M.I. Garcia-Cruz, J.A. Gutierrez-Uribe, J.A. Benavides-Lozano and C. Hernandez-Brenes,2013Persea americana) lipid derivatives that inhibit Clostridium sporogenes endospore germination.]]>6174037411