Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2016.175.180RohmanAbdul LumaksoFajar A. RiyantoSugeng 22016102The falsification of high price edible oils such as avocado oil become a serious problem in the oil trade. Simple and rapid methods for identifying and discriminating authentic Avocado Oil (AO) from its adulterants are increasing demand. This current work proposes the use of combination of mid infrared spectroscopy technique with Partial Least Square-Discriminant Analysis (PLS-DA) as direct and rapid tool to discriminate authentic AO from the adulterants of Soybean Oil (SO) and Grape Seed Oil (GSO) in binary and ternary mixture. Discrimination was carried out using the chemometrics approach, namely PLS-DA based on the infrared spectral data. The selected frequency used for authentication of AO was 1500-750 cm1. Special spectral treatment of smoothing and offset correction was implemented to reduce the noise and background spectral. Furthermore, the high accuracy and clear discrimination were obtained with 100% correctness for binary mixture analysis and 93.3% for ternary mixture.]]>Alexa, E., A. Dragomirescu, G. Pop, C. Jianu and D. Dragos,200972024Aouidi, F., N. Dupuy, J. Artaud, S. Roussos, M. Msallem, I. Perraud-Gaime and M. Hamdi,2012Olea europaea leaves.]]>131360366Aparicio, R. and R. Aparicio-Ruiz,200088193104Ashworth, V.E.T.M., H. Chen and M.T. Clegg,20112011pp: 173-190pp: 173-190Barker, M. and W. Rayens,200317166173Broadhurst, D.I. and D.B. Kell,20062171196Chiavaro, E., E. Vittadini, M.T. Rodriguez-Estrada, L. Cerretani, L. Capelli and A. Bendini,200916227244Fang, G., J.Y. Goh, M. Tay, H.F. Lau and S.F.Y. Li,201313814611469Flores, G., M.L.R. del Castillo, G.P. Blanch and M. Herraiz,200697336342Giacomelli, L.M., M. Mattea and C.D. Ceballos,200683303308Hai, Z. and J. Wang,2006119449455Lee, D.S., B.S. Noh, S.Y. Bae and K. Kim,1998358163175Lee, D.S., E.S. Lee, H.J. Kim, S.O. Kim and K. Kim,2001429321330Lumaksoa, F.A., S. Riyanto, S. Ahmad, A.S. Rosman, F.M. Yusoff and A. Rohman,201531217Marina, A.M., Y.B.C. Man, S.A.H. Nazimah and I. Amin,2009165061Nunes, C.A.,201460255261Nurrulhidayah, A.F., Y.B.C. Man, H.A. Al-Kahtani and A. Rohman,2011Nigella sativa seed oil.]]>25243250Oliveri, P., M.A. Baldo, S. Daniele and M. Forina,200939511351143Quinones-Islas, N., O.G. Meza-Marquez, G. Osorio-Revilla and T. Gallardo-Velazquez,201351148154Requejo, A.M., R.M. Ortega, F. Robles, B. Navia, M. Faci and A. Aparicio,200357S54S57Rohman, A., I. Gupitasari, P. Purwanto, K. Triyana, A.S. Rosman, S.A.S. Ahmad and F.M. Yusof,201469113119Rohman, A. and Y.B.C. Man,201247113Rohman, A. and R. Ariani,2013Nigella sativa seed oil in binary and ternary mixtures with corn oil and soybean oil using FTIR spectroscopy coupled with partial least square.]]>2013Rohman, A. and Y.B. Che Man,200916618628Rohman, A. and Y.B.C. Man,201043886892Rohman, A. and Y.B.C. Man,2011129583588Man, Y.B.C. and A. Rohman,2013159Rohman, A., Sudjadi, Devi, D. Ramadhani and A. Nugroho,2015Curcuma longa and Curcuma xanthorriza using FTIR spectroscopy and chemometrics.]]>9179186Takenaga, F., K. Matsuyama, S. Abe, Y. Torii and S. Itoh,200857591597Vazquez, P.P., M.M. Galera, A.G. Frenich and J.L.M. Vidal,2000164955Vlachos, N., Y. Skopelitis, M. Psaroudaki, V. Konstantinidou, A. Chatzilazarou and E. Tegou,2006573-574459465Westerhuis, J.A., H.C.J. Hoefsloot, S. Smit, D.J. Vis and A.K. Smilde et al.,200848189Yan, Y.L., L.L. Zhao, D.H. Han and S.M. Yang,2005pp: 1-3pp: 1-3