Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2012.511.520Ricinus communis Seeds Extract Using Lipid Peroxidation and Free Radical Scavenging Methods]]>OloyedeGaniyat K.7201267The antioxidant activity of two methanolic fractions (C1, C2) of R. communis
was determined by three methods: Lipid peroxidation by ferric thiocyanate method
and free radical scavenging effect on 2, 2-diphenyl-1-picrylhydrazyl radical
(DPPH) and hydroxyl radical generated from hydrogen peroxide. C1 and C2 at various
concentration possessed significant antioxidant activity (p<0.05) when compared
with antioxidant standards Butylated Hydroxy Anisole (BHA), ascorbic acid and
α-tocopherol used in the assay. C1 had percentage inhibition of 93.98%
while C2 gave 90.10% inhibition at 0.8 mg mL-1 in the lipid peroxidation/ferric
thiocyanate test. In the DPPH assay, C1 had inhibition of 73.71% while C2 gave
87.92% at 1.0 mg mL-1. While in the hydroxyl radical scavenging assay,
the inhibition of C1 was 85.07% while that of C2 was 94.91% at 0.1 mg mL-1,
both extracts therefore, showed comparative antioxidant activities at the concentrations
used. C1 golden brown coloured oil was analysed using gas chromatography/gas
chromatography-mass spectrometry. Four components were obtained, Methyl ricinoleate
(46.68%), Ricinoleic acid (34.41%), (Z, Z)-9, 12-Octadecadienoic acid (12.99%)
and (Z, Z)-9, 12-Octadecadienoic acid, methyl ester (5.92%). These chemical
constituents were assumingly responsible for the observed antioxidant activities
of methanolic extracts from R. communis seeds.]]>Andrew, P.,20093rd Edn.,pp: 56-86pp: 56-86Ansari, J.A., M. Sayyed and F. Sayeed,20117579588Bors, W. and M. Saran,1991266668Boye, G.L. and O. Ampofo,19901990pp: 32-33pp: 32-33Brickell, C.,1996pp: 884-885pp: 884-885Cragg, G.M., D.J. Newman and K.M. Snader,1997605260Darmstadt, G.L., M. Mao-Qiang, E. Chi, S.K. Saha and V.A. Ziboh et al.,200291546554Devendra, C. and G.V. Raghavan,1978141127Duke, J.A. and K.K. Wain,1981Vol. 3,Duke, J.A.,19781978pp: 1-61pp: 1-61Egwuche, R.U., A.A. Odetola and O.L. Erukainure,2011Peperomia pellucida L.]]>54853Ganesh, S. and J.J. Vennila,2011Acanthus ilicifolius and Avicennia officinalis by GC-MS.]]>56065Gaydou, A.M., L. Menet, G. Ravelojaona and P. Geneste,198237135141Gulcin, I., M. Oktay, O.I. Kufrevioglu and A. Aslan,2002Cetraria islandica (L.) Ach.]]>79325329Gupta, V.K. and S.K. Sharma,2010In vitro antioxidant activities of aqueous extract of Ficus bangalensis Linn. Root.]]>4134140Halliwell, B. and J.M. Gutteridge,1984219114Heinrich, M., J. Barnes, S. Gibbons and E.M. Williamson,2004Koleva, I.I., T.A. van Beck, J.P. Linssen, A. de Groot and L.N. Evstaliva,200213817Krishna, K.L., K. Mruthunjaya and J.A. Patel,2009Justicia gendarussa burm.]]>399110Letawe, C., M. Boone and G.E. Pierard,1998235658Lugasi, A., P. Horvahovich and E. Dworschak,1999in vitro antioxidant activity of Ginkgo biloba.]]>13160162Mutee, A.F., S.M. Salhimi, M.F. Yam, C.P. Lim and G.Z. Abdullah et al.,2010In vivo anti-inflammatory and in vitro antioxidant activities of Peperomia pellucida.]]>6686690Namiki, M.,199029273300Oloyede, G.K., P.A. Onocha, J. Soyinka, O.W. Oguntokun and E. Thonda,20101114120Oloyede, K.G. and O.E. Farombi,2010Crinum ornatum bulb extract.]]>53236Oloyede, K.G., M.J. Oke, Y. Raji and A.T. Olugbade,2010Crinum ornatum bulb extract.]]>52631Onocha, P.A., G.K. Oloyede and Q.O. Afolabi,2011Dacryodes edulis).]]>5143149Reed, C.F.,19761976Sharma, R.K., P.K. Chauhan and A. Fulia,20117821828Shuid, A.N., L.K. Siang, T.G. Chin, N. Muhammad, N. Mohamed and I.N. Soelaiman,2011Eurycoma longifolia in male rats.]]>7641646Sies, H.,19971997pp: 3-18pp: 3-18Simpson, B.B. and M. Conner-Ogorzaly,19952nd Edn.,pp: 742-760pp: 742-760Smith, G.C., M.S. Clegg, C.L. Keen and L.E. Grivetti,1996474153Soare, J.R., T.C.P. Dinis, A.P. Cunha and L. Almeida,1997Thymus zygis.]]>26469478Vieira C., S. Evangelista, R. Cirillo, A. Lippi, C.A. Maggi and S. Manzini,20009223228Wedin, G.P., J.S. Neal, G.W. Everson and E.P. Krenzelok,19864259261Yen, G.C. and P.D. Duh,199442629632Sawadogo, W.R., R. Boly, M. Lompo, N. Some, C.E. Lamien, I.P. Guissou and O.G. Nacoulma,2006Dicliptera verticillata.]]>2435438Srivastava, J., J. Lambert and N. Vietmeyer,19961996