Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2012.318.325In vitro Regeneration of Aconitum balfourii Stapf: A Rare Medicinal Herb from Himalayan Alpine Through Root Explants]]>SharmaEti GaurKhushhaal PunethaHimanshu GaurA.K. 4201264Aconitum balfourii Stapf is a rare medicinal herb belonging to genus Aconitum and family Ranunculaceae. Excessive illegal collection from wild due to pharmacological significance along with quiescent nature of seeds and lengthy cultivation cycle are collectively responsible for its endangered status. Therefore, conservation applying tissue culture is a vital need and has been developed nowadays. In the present study, in vitro regeneration of A. balfourii from root explants has been attempted. Root explants were cultured on MS-Medium supplemented with different combinations of BA and NAA and callus induction took place at 13.4 μM NAA and 5.55 μM BA. Such induced calluses were transferred to shooting media. The best shooting were observed in 8.88 μM BA and 0.54 μM NAA. These shoots were subculture on 7.99 μM BAP. Furthermore the result showed that increasing the concentration of BA caused a reducing length of shoots; also presence of low concentration of NAA has been necessary for shoot induction. Some factors such as shoot length, no. of shoots and induction frequencies of shooting and rooting were also studied. The well developed micro shoots were cultured on rooting media for complete regeneration of plant. Rooting of micro shoots occurred in media containing 1.43 μM IAA and 1.23 μM IBA.]]>Ameri, A.,1998Aconitum alkaloids on the central nervous system.]]>56211235Bist, R., K. Pathak, D. Hatwal, H. Punetha and A.K. Gaur,2011In vitro propagation of Aconitum balfourii Stapf: A rare medicinal herb of the alpine Himalayas.]]>68394398Chaudhary, L.B. and R.R. Rao,1998Aconitum L. (Ranunculaceae) in North-West Himalaya (India).]]>109527537Chopra, R.N., R.L. Badhwar and S. Ghosh,1984Vol. I,CMAP,20032003Gaur, R.P.,1999Giri, A., P.S. Ahuja and P.V.A. Kumar,1993Aconitum heterophyllum Wall.]]>32213218Kadota, Y.,1987Aconitum subgenus Aconitum (Ranunculaceae) of East Asia.]]>Pages: 249Pages: 249Khetwal, K.S.,2007Aconitum balfuorii.]]>4613641366Morton, L. and J. Browse,1991Arabidopsis.]]>10235239Murashige, T. and F. Skoog,196215473497Nautiyal, B.P., V. Prakash, R. Bahuguna, U. Maithani, H. Bisht and M.C. Nautiyal,200243297303Jabeen, N., A.S. Shawl, G.H. Dar, A. Jan and P. Sultan,2006Aconitum heterophyllum- medicinal plant.]]>5287291Pandey, H., S.K. Nandi, A. Kumar, U.T. Palni, B. Chandra and L.M.S. Palni,2004In vitro propagation of Aconitum barfourii Stapf: An important aconite of Himalayan alpine.]]>793441Ranjan, R., S.S. Purohit and V. Prasad,2003Pages: 245Pages: 245Samant, S.S., U. Dhar and L.M.S. Palni,1998pp: 163pp: 163Samantary, S. and S. Maiti,2010In vitro organogenesis in Aloe barbadensis Mill.: An aloin a rich plant.]]>678084Watad, A.A., M. Kochba, A. Nissima and V. Gaba,1995Aconitum napellus micropropagation by liquid culture on floating membrane rafts.]]>14345348