Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2011.717.727Aspilia mossambicensis, Ocimum gratissimum and Toddalia asiatica Extracts on Selected Pathogenic Bacteria]]>MunyendoW.L.L. OrwaJ.A. RukungaG.M. BiiC.C. 6201156Aspilia mossambicensis (Oliv.) Wild, Ocimum gratissimum (L.) Labiatae. and Toddalia asiatica (L.) Lam. (Rutaceae) were investigated with an overall aim of identifying and evaluating the bioactive antibacterial agents. Hexane, ethyl acetate and methanol realized yields of the range 0.5% Ocimum gratissimum stem bark ethyl acetate extract to 2.7% for Toddalia asiatica root bark methanol extract. The extracts were assayed for in vitro activity against gram positive; Methicillin Resistant Staphylococcus aureus and the gram negative; Pseudomonas aeruginosa, using disc diffusion method. The highest activity was with T. asiatica stem bark methanol extract (15 mm diameter zone of inhibition) against Methicillin Resistant Staphylococcus aureus. Bioactivity-guided fractionation of the T. asiatica (stem bark methanol extract) yielded up to four isolates labeled F1-C, F2-C, F3-C and F4-C. Preliminary phytochemical analysis gave positive results for mostly alkaloids, flavonoids, steroids and amines. By bioautographic selection the isolates showed antibacterial activity against Methicillin Resistant Staphylococcus aureus (0.3125 mg mL-1) which compared well to that of the standard drug gentamycin (0.5 mg mL-1).These results validate the ethno botanical use of Toddalia asiatica, a Kenyan medicinal plants for conditions that may be of bacterial etiology.]]>Adebolu, T.T. and S.A. Oladimeji,2005Ocimum gratissimum on selected diarrhea causing bacteria in Southwestern Nigeria.]]>4682684Adeniyi, B.A. and R.O. Odufowora,2000In vitro antimicrobial properties of Aspilia africana (compositae).]]>3167170Blasi, F.,200424171181Bourrel, C., F. Perineau, G. Michel and J.M. Bessiere,1993Nepeta cataria L.) essential oil: Analysis of chemical constituents, bacteriostatic and fungistatic properties.]]>5159167Calixto, J.B.,200033179189Campo, S.M., A. Zullo, C. Hassan and S. Morini,2007Helicobacter pylori infection.]]>21117Chanda, S., M. Kaneria and R. Nair,2011Psoralea corylifolia L. seed and aerial parts with various extraction methods.]]>60124131Coulidiati, T.H., H. Millogo-Kone, A. Lamien-Meda, M. Yougbare-Ziebrou, J. Millogo-Rasolodimby and O.G. Nacoulma,2011Combretum species from burkina faso.]]>54253Cowan, M.M.,199912564582Elvin-Lewis, M.,200175141164Jana, S. and G.S. Shekhawat,2010in vivo and in vitro extracts of Indian medicinal herb: Anethum graveolens.]]>4206212Harborne, J.B.,19983rd Edn.,Pages: 302Pages: 302Iwu, M.M., A.R. Duncan and C.O. Okunji,19991999pp: 457-462pp: 457-462Jabra-Rizk, M.A.,20062006Kokwaro, J.O.,19932nd Edn.,Pages: 401Pages: 401Kristjansson, M., B. Viner and J.N. Maslow,199426411416Mariita, R.M., C.K.P.O. Ogol, N.O. Oguge and P.O. Okemo,201155464Oketch-Rabah, H.A., J.W. Mwangi, J. Lisgarten and E.K. Mberu,2000Toddalia asiatica roots.]]>71636640Olila, D., Olwa-Odyek and J. Opuda-Asibo,2001Zanthoxylum chalybeum and Warburgia ugandensis, Ugandan medicinal plants.]]>16672Rajakaruna, N., C.S. Harris and G.H.N. Towers,200240235244Shailaja, V.V., L.A. Pai, D.R. Mathur and V. Lakshmi,2004222833Sharifi-Mood, B., R. Alavi-Naini, M. Salehi, M. Hashemi and F. Rakhshani,20062713621366Sharma, P.N., A. Shoeb, R.S. Kapil and S.P. Popli,1981Toddalia asiatica.]]>20335336Tomas-Barberan, F., E. Iniesta-Sanmartin, F. Tomas-Lorente and A. Rumbero,1990Helichrysum species.]]>2910931095Todar, K.,20022002Tsai, I.L., M.F. Wun, C.M. Teng, T. Ishikawa and I.S. Chen,1998Toddalia asiatica.]]>4813771382Vaghasiya, Y. and S. Chanda,2010Mangifera indica L. seeds.]]>512071212Karim, A., M.N. Sohail, S. Munir and S. Sattar,20117419439Nostro, A., M.P. Germano, V. D'Angelo, A. Marino and M.A. Cannatelli,200030379384