Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2011.557.566Hibiscus sabdariffa Calyx]]>Prosper AnokwuruChinedu EsiabaIjeoma AjibayeOlusola AdesuyiAyobami O. 5201155The polyphenolic content and antioxidant activity of Hibiscus sabdariffa calyx in methanol, ethanol, acetone and water extract were studied. The Total Phenolic Content (TPC) was determined using folin Ciocalteu method while the Total Flavonoid Content (TFC) was determined using aluminum chloride method. Antioxidant activities were determined using 2,2-diphenyl-1-picryl hydrazine (DPPH) inhibition and lipid peroxidation inhibition. Methanol extracted the highest total phenolic content (29.2 mg GAE/g DW) and was significantly different (p<0.05) from the water and acetone extracts only. Acetone extracted the highest flavonoid content (53.6 mg QE/g DW) and was significantly different from the other solvents used. Methanol extract gave the highest inhibition to DPPH (78%) and was only significantly different (p<0.05) from acetone and water extracts. Ethanol gave the highest inhibition to lipid peroxidation (26%) but was not significantly different (p<0.05) from the other solvent extracts. There was a stronger correlation obtained between TPC and inhibition of DPPH (r = 0.969) compared to TFC and DPPH (r = 0.742). The study showed that methanol and ethanol were better solvents for the extraction of phenols of Hibiscus sabdariffa calyx compared to water and acetone. It also showed that phenols contributed more to the antioxidant activity of Hibiscus sabdariffa calyx compared to flavonoids.]]>Baskar, R., V. Rajeswari and T.S. Kumar,2007In vitro antioxidant studies in leaves of Annona species.]]>45480485Braughler, J.M., L.A. Duncan and R.L. Chase,19862611028210289Cai, Y., Q. Luo, M. Sun and H. Corke,20047421572184Christian, K.R. and J.C. Jackson,2009Hibiscus sabdariffa) during maturity.]]>22663667D'Abrosca, B., S. Pacifico, G. Cefarelli, C. Mastellone and A. Fiorentino,200710413331337Dewanto, V., X. Wu, K.K. Adom and R.H. Liu,20025030103014Es-Safi, N.E., J. Bauhaire, C.L. Guerneve and P.H. Ducrot,20072618629Ghimeray, A.K., C. Jin, B.K. Ghimine and D.H. Cho,2009830843091Hatano, T., R. Edamatsu, M. Hiramatsu, A. Mori and Y. Fujita et al.,19893720162021Huda-Faujan, N., A. Noriham, A.S. Norrakiah and A.S. Babji,20098484489Jakopic, J., R. Veberic and F. Stampar,2009931115Jang, H.D., K.S. Chang, Y.S. Huang, C.L. Hsu, S.H. Lee and M.S. Su,2007103749756Joshi, H. and M. Parle,2006Hibiscus sabdariffa linn.]]>51520Josiah, S.J., O. Omotuyi, K.A. Oluyemi, U.I. Ezea and E.S. Uhunmwangho et al.,2010Hibiscus sabdariffa calyx against potassium bromated induced tissue damage in winster rats.]]>932183222Kahl, R. and H. Kappus,1993196329338Kaur, R., S. Arora and B. Singh,2008Chukrasia tabularis A. Juss.]]>9976927698Koffi, E., T. Sea, Y. Dodehe and S. Soro,20105550558Mahadevan, N.S. and K. Pradeep,2009Hibiscus sabdariffa: An overview.]]>87783Mensor, L.L., F.S. Menezes, G.G. Leitao, A.S. Reis, T.C. dos Santos, C.S. Coube and S.G. Leitao,200115127130Miller, N.J. and M.B. Ruiz-Larrea,2002123951Mohd-Esa, N., F.S. Hern, A. Ismail and C.L. Yee,2010Hibiscus sabdariffa L.) extracts and potential exploitation of the seeds.]]>12210551060Mudgal, V., N. Madaan, A. Mudgal and S. Mishra,20105154162Oboh, G.,2008Hibiscus sabdariffa calyx) and green (Camellia sinensis) teas on some pro-oxidant induced oxidation stress in brain.]]>14049Ochani, P.C. and P. D`Mello,2009Hibiscus sabdariffa Linn. Leaves and calyces extracts in rats.]]>47276282Ruberto, G., M.T. Baratta, S.G. Deans and H.J. Dorman,2000Foeniculum vulgare and Crithmum maritimum essentials oils.]]>66687693Singleton, V.L., R. Orthofer and R.M. Lamuela-Raventos,19991999pp: 152-178pp: 152-178Singleton, V.L. and J.A. Rossi,196516144158Subhasree, B., R. Baskar, R.L. Keerthana, R.L. Susan and P. Rajasekaran,200911512131220Sultana, B., F. Anwar and M. Ashraf,20091421672180Tawaha, K., F.Q. Alali, M. Gharaibeh, M. Mohammad and T. El-Elimat,200710413721378Veeru, P., M.P. Kishor and M. Meenakshi,20093608612Wong, S.P., L.P. Leong and J.H.W. Koh,200699775783Yao, L., Y. Jiang, N. Datta, R. Singanusong and X. Liu et al.,2004Camellia sinensis) grown in Australia.]]>84253263Zheng, W. and S.Y. Wang,20014951655170Kubola, J. and S. Siriamornpun,2008Momordica charantia L.) leaf, stem and fruit fraction extracts in vitro.]]>110881890Cao, G., E. Sofic and R.L. Prior,199722749760Blois, M.S.,195818111991200