Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2011.330.337In vitro Validation and Phyto-constituent Analysis of Turmeric Extract: An Ethnological Alternative for Eye Treatment]]>SinghS. SathpathyB.S. SahooR.K. SubudhiE. NayakS. 3201153The essential oil from the rhizomes of Roma cultivar of turmeric (Curcuma longa) from Orissa was examined for its antimicrobial activity against the pathogens causing eye infections. The purpose of this study was to authenticate the use of turmeric rhizome oil against eye infections so as to giving an approach to formulate turmeric rhizome oil as potential eye drop in place of traditional antibiotics after undertaking its in vivo pharmacological studies. Essential oil from rhizomes of Roma cultivar obtained by hydrodistillation extraction method using clenevger apparatus. The antimicrobial effects of oil towards Staphylococcus aureus, Pseudomonas aerugenosa, Candida albicans and Aspergillus niger were tested by Inhibition Zone Diameter (IZD) test to screen the antimicrobial activity, Minimum Inhibitory Concentration (MIC) test and Minimum Killing Time (MKT) test to determine the minimum concentration of oil and minimum time required to kill the pathogens. Oil showed very good activity against all four microbial strains used at concentration of 10 μL except Pseudomonas sp. Very low concentration of 1.95 μL mL-1 oil was needed to inhibit the growth of most highly infecting pathogen Staphylococcus aureus within 15 min of its exposure in comparison to other microbial strains. High turmerone content (49.76%) of elite turmeric cultivar Roma released from Orissa (India) might be assigned to be responsible for such excellent anti microbial activity against the tested pathogens.]]>Ajaiyeoba, E.O., W. Sama, E.E. Essien, J.O. Olayemi, O. Ekundayo, T.M. Walker and W.N. Setzer,2008Curcuma longa leaf and rhizome from Nigeria on Anopheles gambiae.]]>46279282Ammon, H.P.T., M.I. Anazodo, H. Safayhi, B.N. Dhawan and R.C. Srimal,19924 formation in rat peritoneal polymorphonuclear neutrophils (PMNL).]]>58226226Amphawan, A., N. Vanittanakom and D. Buddhasukh,1995Curcuma longa (Zingiberaceae).]]>49163169Anonymous,1979Vol. 33,pp: 275-83pp: 275-83Araujo, C.A.C. and L.L. Leon,2001Curcuma longa L.]]>96723728Baratta, M.T., H.J.D. Dorman, S.G. Deans, A.C. Figueiredo, J.G. Barroso and G. Ruberto,199813235244Chattopadhyay, I., K. Biswas, U. Bandyopadhyay and R.K. Banerjee,2004874453Chopra, R.N., J.C. Gupta and G.S. Chopra,194129769772Cosgrove, S.E., G.A. Vigliani, Jr. V.G. Fowler, E. Abrutyn and G.R. Corey et al.,200948713721Duke, J.A.,2003Eigner, D. and D. Scholz,1999Ferula asa-foetida and Curcuma longa in traditional medical treatment and diet in Nepal.]]>6716Groden, L.R., B. Murphy and J. Rodnite,1991105053Guenther, E.,1948Vol. 1,pp: 316-318pp: 316-318Gul, N., T.Y. Mujahid, N. Jehan and S. Ahmad,2004Curcuma longa against urinary tract infection isolates.]]>720552060Jorge, A.,2004633.8395403Kaur, G.J. and D.S. Arora,200993030Kim, H.S.,20051003739Babu, G.D.K., V. Shanmugam, S.D. Ravindranath and V.P. Joshi,2007Curcuma longaL. leaf oils produced by different water distillation techniques.]]>22191196Lowy, F.D.,1998Staphylococcus aureus infections.]]>339520532Mahady, G.B., S.L. Pendland, G. Yun and Z.Z. Lu,2002Curcuma longa) and curcumin inhibit the growth of Helicobacter pylori, of group 1 carcinogen.]]>2241794181Iwu, M.M.,19931st Edn.,pp: 464pp: 464Pattnaik, S., V.R. Subramanyam and C.C. Rath,1995E. coli (SP-11).]]>84195199Pattnaik, S., V.R. Subramanyam, M. Bapuji and C.R. Kole,1997893946Poole, K.,2004101226Prabu, G.R., A. Gnanmamni and S. Sadulla,2006Streptococcus mutans.]]>101487495Prakash, L., K.S. Satyan and S. Majeed,20031184146Ravindran, P.N., N.K. Babu and K. Sivaraman,2007Curcuma.]]>Rath, C.C., S. Mishra, S.K. Dash and R.K. Mishra,200542797801Stapleton, F., J.K. Dart, D.V. Seal and M. Matheson,1995Pseudomonas aeruginosa keratitis in contact lens wearers.]]>114395402Singh, R., R. Chandra, M. Bose and P.M. Luthra,2002Curcuma longa rhizome extract on pathogenic bacteria.]]>83737740Singh, G., S. Maurya, C.A.N. Catalan and M.P. de Lampasona,2005Curcuma longa essential oil and its oleoresin.]]>49441451Srimal, R.C. and B.N. Dhawan,19851985Usman, L.A., A.A. Hamid, O.C. George, O.M. Ameen, N.O. Muhammed, M.F. Zubair and A. Lawal,2009Curcuma longa L. growing in North Central Nigeria.]]>4178181