Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2010.197.205 Terminalia muelleri Benth. Leaves]]>AnamK. SugandaA.G. SukandarE.Y. KardonoL.B.S. 4201044The objective of this study was to know the antibacterial compound from T.muelleri. Extraction of leaf of T. muelleri using solvents of increasing polarity, namely, n-hexane, ethyl acetate and methanol, respectively, yielded dry extracts. The extracts were tested for antibacterial activity. Ethyl acetate extract exhibited the strongest activity against standard strains of Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. The ethyl acetate extracts had been fractionated, yielded 9 fractions.The fraction EH exhibited the strongest activity against S. aureus. The separation of chemical contents of fraction EH was carried out by Sephadex LH-20 column chromatography, yielded a pure substance. The substances was identified as 3,4,5-trihidroxybenzoic acid by chromatographic and spectroscopic analysis. This substance is a well-known antibacterial activities. The activity of 1 mg 3,4,5-trihydroxybenzoic acid was equal to 0.1396 μg tetracycline HCl against S. aureus and 0.6455 μg against Methicilin-Resistant Staphylococcus aureus, also equals to 40.6035 μg penicillin G against S. aureus and equals to 2.9823 and 2.1213 μg vancomycin HCl against S. aureus and Methicilin-Resistant Staphylococcus aureus, respectively.]]>Akiyama, H., K. Fujii, O. Yamasaki, T. Oono and K. Iwatsuki,2001Staphylococcus aureus.]]>48487491Anam, K., A.G. Suganda, E.Y. Sukandar and L.B.S. dan Kardono,2009Terminalia muelleri Benth. leaves.]]>74043Betina, V.,1973784151Chanwitheesuk, A., A. Teerawutgulrag, J.D. Kilburn and N. Rakariyatham,2007Caesalpinia mimosoides Lamk.,]]>10010441048Goun, E., G. Cunningham, D. Chu, C. Nguyen and D. Miles,200374592596Kandil, F.E. and M.I. Nassar,1998Terminalia arjuna.]]>4715671568Kroes, B.H., A.J.J. van den Berg, H.C.Q. van Ufford, H. van Dijk and R.P. Labadie,199258499504Masoko, P., J. Picard and J.N. Eloff,2005Terminalia species (Combretaceae).]]>99301308Kawada, M., Y. Ohno, Y. Ri, T. Ikoma, H. Yuugetu and T. Asai,200112847852Moshi, M.J. and Z.H. Mbwambo,2005Terminalia sericea roots.]]>974347Muschietti, L., M. Derita, V. Sulsen, J. de Dios Munoz, G. Ferraro, S. Zacchino and V. Martino,2005In vitro antifungal assay of traditional argentine medicinal plants.]]>102233238Panizzi, L., C. Caponi, S. Catalano, P.L. Cioni and I. Morelli,2002In vitro antimicrobial activity of extracts and isolated constituents of Rubus ulmifolius.]]>79165168Penna, C., S. Marino, E. Vivot, M.C. Cruanes and J.D. Munoz et al.,2001Sebastiania brasiliensis.]]>773740Shukla, Y.N., A. Srivastava, S. Kumar and S. Kumar,1999Argyreia speciosa and Oenothera biennis.]]>67241245Srivastava, S.K., S.D. Srivastava and B.K. Chouksey,2001Terminalia alata.]]>72106112Suganda, A.G., E.Y. Sukandar and L. dan Ratna,2006Terminalia species.]]>311823Suganda, A.G., E.Y. Sukandar and R.S. Hardhiko,2004Terminalia catappa L. Leaves.]]>29129133Tang, X.H., J. Gao, Y. Wang, Y.M. Fan and L.Z. Xu et al.,2006Terminalia catappa L. leaves from damage induced by carbon tetrachloride in liver mitochondria.]]>17177182Tyler, E.V., J.E. Robber and L.R. Brady,19889th Edn.,pp: 312-318pp: 312-318Lemmens, R.H.M.J. and N. Wulijarni-Soetjipto,1992pp: 23pp: 23