Research Journal of Medicinal Plants1819-34552151-7924Science International10.3923/rjmp.2008.1.9 Naravelia zeylanica (L.) DC.]]>NaikaH. RajaKrishnaV. 1200821An in vitro regeneration protocol was standardized using leaf explants of Naravelia zeylanica (Ranunculaceae) a rare medicinal plant of the Western Ghats, India. The adventitious shoot buds were organized directly from mid veins and margin of the excised leaf explants cultured on MS-medium fortified with a range of 2.0 to 3.0 mg L-1 BAP and 0.3 to 0.7 mg L-1 IBA. The frequency of shoot bud organogenesis was the highest (14.9±0.27 shoots per explants) at the concentration of 2.5 mg L-1 BAP and 0.5 mg L-1 IBA. The excised micro shoots were pretreated in 0.5 mg L-1 IBA and transferred to MS half strength semisolid medium induced root initials from the cut ends. A mean of 13.5±0.30 root intact plantlets was recovered per explants showed morphological similarity with the in vivo plants. Two hundred and fifty gram of the powdered leaves from in vivo plants of N. zeylanica was subjected to Soxhlet extraction using methanol. Ten gram of each of the extracts were used for total alkaloid isolation and the yield was 270 mg. An alkaloid berberine was isolated by preparative TLC method using the solvent system methanol, water and ammonium hydroxide in the ratio 8: 1: 1 v/v. The characterization of the constituent was confirmed by IR, 1HNMR and Mass spectral studies.]]>Anis, K.V., N.V. Rajeshkumar and R. Kuttan,200153763768Bansal, Y.K. and P. Pandey,1993Sesbania acculeata Pers. adventitious organogenesis.]]>32351355Biondo, R., A.M. Soares, Bertoni, S.C. Franca and A.M.S. Pereira,2004Mandevilla illustris (Vell) woodson and effects of its aqueous extract on the enzymatic and toxic activities of Crotalus durissus terrificus snake venom.]]>22549552Biswas, T.K. and B. Mukherjee,200322539Duncan, D.B.,195511142Emma, M.J., A. Sevdalina and A.C.J. Verkleij,2005Silene vulgaris (Caryophyllaceae).]]>802531Faisal, M. and M. Anis,2003Tylophora indica Merrill via leaf callus culture.]]>75125129Gilani, A.H. and K.H. Janbaz,1992Berberis aristata leaves.]]>20347S347SHarsha, V.H., S.S. Hebbar, V. Shripathi and G.R. Hegde,2003843740Ludwig-Muller, J.,200032219230Kokate, C.K., A.P. Purohith and S.B. Gokhale,1990Lau, C.W., X.Q. Yao, Z.Y. Chen, W.H. Ko and Y. Huang,200119234244Li, J., M. Jain, R. Vunsh, J. Vishnevetsky, U. Hanaia and M. Flaishman, A. Perl and M. Edelman,2004Spirodela and Lemna.]]>22457464Manickam, V.S., R.E. Mathavan and R. Antonisamy, 200062181185Cernokova, M., D. Kostalava, V. Kettmann, M. Plodova, J. Toth and J. Drimal,2002Mahonia aquifolium.]]>222Mishra, M.K. and H.L. Sreenath,2003In vitro induction of adventitious shoot buds and plant regeneration from leaf explants of Coffea bengalensis Roxb -A wild coffee species.]]>45762Murashige, T. and F. Skoog,196215473497Nickell, G.L. and O. Kirk,1982Pi, L.T. and T.H. Tsai,200432405412Praveendhar, T. and S.N. Ashalatha,2003In vitro regeneration and detection of berberine from Naravelia zeylanica.]]>4155160Rani, G. and I.S. Grover,1999In vitro callus induction and regeneration studies in Withania somnifera.]]>572327Ravikumar, K. and D.K. Ved,2000pp: 123-126pp: 123-126Rout, G.R., S. Samantaray and P. Das,2000In vitro manipulation and propagation of medicinal plants.]]>1891120Saldanha, C.J. and D.J. Nicolson,1976Saldanha, C.J.,1984Scaramuzzi, F. and S.D. Emerico,2003Clerodendrum fragrans and stability of ploidy level of in vitro plantlets.]]>5416Shankarmurthy, K., V. Krishna, K.R. Maruthi and B.A. Rahiman,2004Embelia ribes burm -A threatened medicinal plant.]]>4917Sivarajan, V.V. and I. Balachadran,1958Sriskandarajah, S.S., Frello and B. Serek,2001in vitro in Campanula carpatica.]]>67295298Vespasiano, B.P., C. Tiagoribeiro and O. Wagner,2003Annatto (Bixa orellana).]]>75159167Warrier, P.K., V.P.K. Nambiar and C. Ramankutty,1995Vol. 4,pp: 100-102pp: 100-102