Research Journal of Immunology1994-79092077-2211Asian Network for Scientific Information10.3923/rji.2011.31.37PremalathaM. DhasarathanP. 1201141Probiotic efficiency of lactobacillus strains was isolated from milk sample and their antibacterial activities against human bacterial pathogens were screened in the present study. The milk samples were collected in sterile containers from different places of Tamil Nadu, India. The samples were analysed qualitatively and microbiologically. Lactobacillus was isolated from the milk samples and it was identified by biochemical tests. Methylene blue reduction test, which is colour sensitive to oxygen concentration is added to the milk. Resazurin test used to screen milk quality based either on the colour production. The total bacterial population count of milk sample was enumerated by Pour plate technique. MRS agar plates were prepared and a loopful of milk sample is taken. Quadrant streak procedure was carried out to isolate Lactobacillus. The isolated strains are identified and used to study their antagonistic efficiency against human bacterial pathogens isolated from different pathologic medium from patients diagnosed to have various wound infection at the laboratory Joys, Nagercoil, Kanyakumari (district) Tamilnadu. The probiotic action of Lactobacillus was studied against some human pathogens like Eschericia coli, Staphylococcus aureus, Enterobacter aerogenes, Streptococcus pyogenes, Pseudomonas aeruginosa and Salmonella typhimurium and it was observed that Lactobacillus showed good antagonistic effect against these pathogens.]]>Klaenhammer, T.R.,2000130415S416SReuter, G.,2001114410419Strus, M., K. Pakosz, H. Gosciniak, A. Przondo-Mordarska and E. Rozynek et al.,2001Lactobacillus bacteria strains against anaerobic gastrointestinal tract pathogens (Helicobacter pylori, Compylobacter coli, Campylobacter jejuni, Clostridium difficile).]]>53133142Nowroozi, J., M. Mirzaii and M. Norouzi,2004Lactobacillus as probiotic bacteria.]]>3317Ghafoor, A., S. Naseem, M. Younus and J. Nazir,2005TM) on broiler chicken vaccinated against avaian influenza virus (H9).]]>4777780Forouhandeh, H., S.Z. Vahed, M.S. Hejazi, M.R. Nahaei and M.A. Dibavar,2010Lactobacillus species from various dairy products.]]>38488Kaila, M., E. Isolauri, E. Soppi, E. Virtanen, S. Laine and H. Arvilommi,1992Lactobacillus strain.]]>32141144Kantele, J.M., H. Arvilommi, S. Kontiainen, M. Salmi and S. Jalkanen et al.,199611010611067Murry, A.C., A. Hinton and H. Morrision,2004Eschericia coli, Salmonella typhimureum, Clostridia perfrigens on chicken feed media by Lactobacillus salivarius and Lactobacillus plantarum.]]>3603607Osuntoki, A.B., O.R. Ejide and E.A. Omonigbehin,2008Lactobacillus isolated from dairy products.]]>7311316Perdigon, G., M.E. de Macias, S. Alvarez, G. Oliver and A.P. de Ruiz Holgado,1998Lactobacillus casei and Lactobacillus acidophilus.]]>631723Quiding-Jarbrink, M., M. Lakew, I. Nordstrom, J. Banchereau, E. Butcher, J. Holmgren and C. Czerkinsky,199525322327Strobel, S. and A.M. Mowat, 199819173181Berg, R.D.,19964430435Helgeland, L., J.T. Vaaga, B. Rolstad, T. Midvedt and P. Brandtzead,199689494501Hoepelman, A.I. and E.I. Tuomanen,19926017291733Hook, M. and L. Switalski,1992Jiang, H.Q., N.A. Bos and J.J. Cebra,20016936113617Lemonier, L.,199631107133Moreau, M.C., R. Ducluzeau, D. Guy-Grand and M.C. Muller,197821532539Salminen, S., M. Deighton, Y. Berno and S.L. Gorbach,19986537633766Sanders,20001311271140Sharma, R. and U. Schumacher,19954025322539Sudo, N., S.A. Sawamura, K. Tanakai, Y. Aiba, C. Kubo and Y. Koga,199715717391745Svanborg, C.,19941994pp: 71-78pp: 71-78Tannode,1995Tournut, J.,1993169645652Gibbs, W.N.,19742311971202Nixon, M.C. and A.B. Lamb,194591823Aggarwal, S.,20062006Trachoo, N. and C. Boudreaux,20066202208Salminen, S., C. Bouley, M.C. Boutron, J.H. Cummings and A. Franck et al.,199880S147S171