Research Journal of Environmental Toxicology1819-3420xxxx-xxxxAcademic Journals Inc.10.3923/rjet.2009.159.169Alcaligenes faecalis Degradation of Chrysene and Diesel Oil with Concomitant Production of Biosurfactant]]>Igwo-EzikpeM.N. GbenleO.G. IloriM.O. OkpuzorJ. OsuntokiA.A. 4200934Alcaligenes faecalis was evaluated for its potential to degrade varying concentrations of chrysene and diesel oil with concomitant biosurfactant production. Biodegradation was set up for 7 days utilizing the substrates as sole carbon and energy sources. Residual chrysene obtained after degradation of 30, 50 and 100 mg L-1, respectively was 17.4±1.5, 27.2±1.2 and 28.7±1.4 mg L-1 while total petroleum hydrocarbon remaining after degradation of 3, 5, 15 and 30% (v/v) diesel oil respectively was 2.58±0.5, 3.09±1.2, 21.65±5.4 and 63.92±8.1%. Microbial cells of A. faecalis and sterilized cell-free extract from diesel oil media showed emulsifying activities against kerosene, diesel oil, engine oil, hexadecane, dodecane, xylene and hexane whereas no emulsifying activity was observed of microbial cells and sterilized cell-free extract from chrysene media. Alcaligenes faecalis cells harvested from diesel oil media also showed haemolytic activity unlike the microbial cells from chrysene media. Growth of the isolate in chrysene and diesel oil media induced secretion of protein and carbohydrate into the media which were statistically significantly (p<0.05) different compared to controls. This study portrays the potential of Alcaligenes faecalis to degrade and grow on chrysene and diesel oil and induce extracellular protein and carbohydrate with concomitant production of biosurfactant for industrial purposes and in hydrocarbon bioremediation.]]>Abdel-El-Haleem, D., 200327174Abouseouda, M., R. Maachi, A. Amranec, S. Boudergua and A. Nabia,2008Pseudomonas fluorescens.]]>223143151Al-Turki, A.I.,2009318Gutnick, D., H. Bach and Y. Berdichevsky,2003Acinetobacter venetianus RAG-1 enhances the emulsifying activity of the polymeric bioemulsifier emulsan.]]>6926082615Barkay, T., S. Navon-Venezia, E. Ron and E. Rosenberg, 19996526972702Bento, F.M., F.A.D.O. Camargo, B.C. Okeke and W.T. Frankenberger,2005160249255Bicca, F.C., L.C. Fleck and M.A.Z. Ayub, 1999Rhodococcus rubber and Rhodococcus erythropolis.]]>30231236Dean-Ross, D., 2005743239Ziel, E., G. Paquette, R. Villemur, F. Lepine, J. Bisaillon,1996Pseudomonas strain growing on polycyclic aromatic hydrocarbons. ]]>6219081912Gogoi, B.K., N.N. Duttaa, P. Goswamia and K.T.R. Mohanb, 20037767782Igwo-Ezikpe, M.N., O.G. Gbenle and M.O. Ilori,20065823828Ilori, M.O.N. and D.I. Amund, 2001Pseudomonas aeruginosa grown on hydrocarbons. ]]>56547552Ilori, M.O. and D.L. Amund,200055890897Jayaraman, J.,1981pp: 53pp: 53Kanaly, R.A. and S. Harayama,200018220592067Lee, M., M.K. Kim, M. Kwon, B.D. Park, M.H. Kim, M. Goodfellow and S. Lee, 2005Gordonia nitida strain LE31. ]]>100429436Lin, S.C., 199666109120Lu, J.R., X.B. Zhao and M. Yaseen,2007126067Makkar, R.S. and K.J. Rockne, 20032222802292Maneerat, S.,20052712631272Masih, A. and A. Taneja,200665449456Moraes, I.D., M. Benincass and R.M. Alegre, 2002Pseudomonas aeruginosa. ]]>5145149Nwachukwu, S.C.U., P. James and T.R. Gurney, 2001Lepidium sp.) after bioremediation of agricultural soil polluted with crude petroleum using adapted Pseudomonas putida. ]]>222936Nwachukwu, S.C.U., 2001Pseudomonas putida, with inorganic nutrient supplementations.]]>42231236Okoh, A.I. and M.R. Trejo-Hernandez,2006525202525Pornsunthorntawee, O., P. Wongpanit, S. Chavadej, M. Abe and R. Rujiravanit, 2008Pseudomonas aeruginosa SP4 isolated from petroleum-contaminated soil. ]]>9915891595Rahman, K.S.M., T.J. Rahman, P. Lakshmanaperumalsamy, R. Marchant and I.M. Banat, 200323335345Saeed, T. and M. Al-Mutairi, 20004141145Sepahy, A.A., M.M. Assadi, V. Saggadian and A. Noohi, 2004Bacilli. ]]>1287293Sikkema, J., J.A. de Bont and B. Poolman,199559201222Tabatabaee, A., M.A. Mazaheri, A.A. Noohi and V.A. Sajadian,20052612Toren, A., S. Navon-Venezia, E.Z. Ron and E. Rosenberg, 20016711021106Volkering, F., A.M. Breure, A. Sterkenburg and J.G. van Andel, 199236548552Willumsen, P.A. and U. Karlson,19967415423Xue, W. and D. Warshawsky,20052067393Yu, S.H., L. Ke, Y.S. Wong and N.F.Y. Tam, 200531149154Zhang, G., Y. Wu, X. Qian and Q. Meng, 2005Pseudomonas aeruginosa in the presence of rhamnolipids. ]]>6725730Igwo-Ezikpe, M.N., O.G. Gbenle and M.O. Ilori,2007Acinetobacter mallei.]]>2007