Research Journal of Environmental Sciences1819-34122151-8238Academic Journals Inc.10.3923/rjes.2010.407.413In vitro Germination and Early Seedling Growth of Zinnia elegans]]>MahmoodzadehH. AbbasiF. RohaniS. 4201044In vitro seeds germination and plantlet establishment of Zinnia elegans were studied in this report. The seeds of Z. elegans were sterilized and cultured on Murashige and Skoog (MS) solid media supplemented with 1-3 μM of 6-benzylaminopurine (BAP) or Kinetin (KIN) and 3% (w/v) sucrose. The presence of KIN in the medium significantly affects seeds germination. High percentage of seeds germination (up to 90%) was successfully achieved after 3 days of culture on medium supplemented with 1 μM KIN. The average of 3.8 leaves per explant obtained on media treated with 3 μM BAP. Result showed that medium supplemented with 2 μM KIN promote the highest growth with an average of 2.72 cm shoot length and 1.6 cm root length after 3 weeks. In addition, medium added with 2 μM BAP and supplemented with 3-4% (w/v) of sucrose promote the best growth i.e., 8.6 mm leaf length. In conclusion, present results showed that it is possible to improve the production of Zinnia elegans plants in vitro with using different cytokinins and carbon sources.]]>Anis, M., H.M. Kashif and S. Anwar,2005In vitro plant regeneration of Pterocarpus marsupium Roxb., an endangered leguminous tree.]]>88861863Auer, C.A., V. Motyka, A. Brezinova and M. Kaminek,1999Petunia hybrida.]]>105141147Bhatt, I.D. and U. Dhar,2004Myrica esculenta Buch.-Ham. Ex D. Don: A high value wild edible of Kumaun Himalaya.]]>3534540Bilyeu, K.D., J.G. Laskey and R.O. Morris,200339195203Chaitanya, K.S.K. and S.C. Naithani,1998Shorea robusta Gaertn f.) seeds at low temperature: Role of kinetin in delaying membrane deterioration during desiccation-induced injury.]]>176369Chiwocha, S.D.S., A.J. Cutler, S.R. Abrams, S.J. Ambrose, J. Yang, A.R.S. Ross and A.R. Kermode,2005Arabidopsis thaliana affects the abscisic acid, auxin, cytokinin and gibberellin metabolic pathways during maintenance of seed dormancy, moist-chilling and germination.]]>423548Galuszka, P., I. Frebort and M. Sebela,200032315327Galuszka, P., I. Frebort, M. Sebela and P. Sauer,2001268450461Gulzar, S. and M.A. Khan,200245617619Khan, M.A. and I.A. Ungar,1997Zygophyllum simplex L. from Pakistan.]]>80395400Khan, M.A., B. Gul and D.J. Weber,2004Ceratoides lanata.]]>823742Mackay, W.A., J.L. Tipton and G.A. Thompson,1995Cercis canadensis var. mexicana.]]>43295299Malik, K.A. and P.K. Saxena,1992Phaseolus acutifolius A., P. aureus L. Wilezek., P. coccineus L. and P. wrightii L.]]>11163168Malik, K.A. and P.K. Saxena,1992Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris).]]>19731740Murashige, T. and F. Skoog,196215473497Neibaur, I., M. Gallo and F. Altpeter,2008Seashore paspalum (Paspalum vaginatum Swartz).]]>44480486Sinha, P. and S.K. Roy,2002in vitro cormel formation from callus culture of Gladiolus primulinus baker.]]>12139145Spichal, L., N.Y. Rakova, M. Riefler, T. Mizuno and G.A. Romanov,2004Arabidopsis thaliana, CRE1/AHK4 and AHK3, differ in their ligand specificity in a bacterial assay.]]>4512991305Stirk, W.A., J.D. Gold, O. Novak, M. Strnad and J. van Staden,2005Tagetes minuta L.]]>4717Stojicic, D. and S. Budimir,2004Pinus heldreichii.]]>48477479Victor, J.M.R., S.J. Murch, S. KrishnaRaj and P.K. Saxena,199928915Werner, T., V. Motyka, M. Strnad and T. Schmulling,2001981048710492Yoon, E.S. and Y.E. Choi,2002Polygonatum odoratum via the culture of seedling explant.]]>43337