Research Journal of Environmental Sciences1819-34122151-8238Academic Journals Inc.10.3923/rjes.2009.357.366Pycnoporus sanguineus Mycelia for Laccase Production and Biodegradation of Polycyclic Aromatic Hydrocarbons]]>Y.S. LowJaime AbdullahNoorlidah VikineswaryS. 3200933The ability of white rot fungus Pycnoporus sanguineus
to colonize support materials and subsequently produce laccase and degradation
of Polycyclic Aromatic Hydrocarbons (PAHs) was compared with free mycelia
culture. Natural support, Ecomat, was found to be the best support material
for P. sanguineus for mycelial colonization and laccase activity
with maximum activity of 39 nkat mL-1 on day nine of incubation.
Coconut husk and grey scouring sponge produces maximum laccase activity
of 9.17 and 6.67 nkat mL-1, respectively on day 15. Pycnoporus
sanguineus immobilized culture exhibited higher PAHs degradation efficiency
compared to the free mycelia culture during the 20 days of incubation.
The immobilized mycelia culture degraded 88% of phenanthrene, 93% of anthracene
and 85% of pyrene within 20 days. The good correlation between the amount
of PAHs degraded and laccase activity produced in the immobilization medium
indicated that laccase was solely responsible for degradation of the three
PAHs tested. In comparison, free mycelia culture rapidly degraded 42%
of phenanthrene, 92% of anthracene and 87% of pyrene at the cessation
of incubation. However, poor correlation between the amount of PAHs degraded
and laccase activity measured in the free cell culture was obtained. This
suggested that intracellular enzymes could be involved in PAHs degradation
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