Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2021.23.28in vitro Growth of Pestalotiopsis mangiferae from Mango Tree]]>MilanMariany D. SouzaDinelí P. H.P.C. MunizPaulo PeixotoGustavo H.S. AhmadSareer GuimarãesGesiane R. OliveiraThiago A.S. A.A. DuarteElizabeth SantosSolange X. RodriguesFabrício CarvalhoDaniel D.C. 12021201Background and Objective: The fungus Pestalotiopsis mangiferae causes a gray leaf spot, which can reduce fruit production. This work aimed to carry out the pathogenic and physiological characterization of Pestalotiopsis mangiferae. Materials and Methods: For the physiological evaluation, isolates were streaked on the Petri dishes containing PDA medium and incubation in BOD chamber at 25°C under three light regimes (0, 12 and 24 h) for 6 days. Evaluations were performed daily with a digital caliper to obtain growth measures. After 6 days of incubation, a total of 10 mL of sterile distilled water was added to each Petri dish for sporulation evaluation. For the pathogenic characterization, the isolates were inoculated in leaves and kept in transparent acrylic boxes. The design was completely randomized, with 6 leaves of Mangifera indica per isolate of P. mangiferae. The lesions were measured at 2, 4, 6, 8 and 10 days of inoculation with the aid of a digital caliper. Results: The N-01-15 isolate was statistically superior to the other isolates in terms of the spotted leaf area and the area under the progress disease curve, all isolates were able to cause injuries to the mango leaves. Regarding physiological evaluation, the daily light hour regime directly influences mycelial growth and spore production of P. mangiferae. Conclusion: The amount of daily light hours directly influences mycelial growth and spore production of P. mangiferae. The isolates were capable of lesions ranging from 0.44-1.46 cm2 in mango leaves.]]>Dar, M.S., P. Carvalho, H. Chidley, A. Deshpande, A. Giri and V. Gupta,2016Mangifera indica L.) cultivars.]]>2016Pages: 445-467Pages: 445-467Pereira, S.C.F., M.R.S. Scarpin and J.F. Neto,202031111Faria, L.N., A.A.S.I. memoriam, S.L.R. Donato, M.R. dos Santos and L.G. Castro,201636387398da Silva Caldana Nathan, F., R.N. Pablo, G.B.F. Luiz, C.M. Alan, H.C. Paulo, V.C.Z. Paulo and A.M. Jorge,2020Mangifera indica) in the hydrographic basin of Paran river III, Brazil.]]>16983991Dias, A.F., V. Giongo, V. da Silva Barros, J.M. Carneiro and M.C.B. de Figueirêdo,20202515881604Lima, L.C., M.S.C. Dias, M.V. de Castro, P.M.R. Júnior and E. de Barros Silva,2007Mangifera indica L.) cv. Haden, after hydrothermic treatment and storage under refrigeratio and in modified atmosphere. ]]>31298304Carvalho, C.,20172017Silva, C.R.R., E.B.A. Fonseca and M.A. Moreira,20022002Silvério, M.L., M.A. de Queiroz Calvacanti, G.A. da Silva, R.J.V. de Oliveira and J.L. Bezerra,2016Neopestalotiopsis from Brazil.]]>28151158Smith, G.J.D., E.C.Y. Liew and K.D. Hyde,2003Xylariales: A monophyletic order containing 7 families.]]>13185218Maharachchikumbura, S.S.N., L.D. Guo, E. Chukeatirote, A.H. Bahkali and K.D. Hyde,2011Pestalotiopsis-morphology, phylogeny, biochemistry and diversity.]]>50167187Carmo, A.L.M., F.A.O. Garcia and F.S.B. Peres,2013Eucalyptus viminalis labill. in Brazil.]]>916341640Lopes, H.V., Á.F. dos Santos, E.D.M.N. Luz and D.J. Tessmann,201945164171Yang, X.L., J.Z. Zhang and D.Q. Luo,2012Pestalotiopsis genus.]]>29622641Maharachchikumbura, S.S.N., K.D. Hyde, J.Z. Groenewald, J. Xu and P.W. Crous,2014Pestalotiopsis revisited.]]>79121186Molen, K.M.V., H.A. Raja, T. El-Elimat and N.H. Oberlies,20132013da Cruz, M.F.A., A.M. Prestes and J.L.N. Maciel,20093915621564Carvalho, D.D.C., E. Alves, T.R.S. Batista, R.B. Camargos and E.A.G.L. Lopes,2009Alternaria alternata from citrus.]]>39792798Ferreira, D.F.,20113510391042, (In Portuguese)Moura, M.A.E., A.M.C. Castilho and M.E. Fraga,201618335349da Silva, J.L. and R.N.V. Teixeira,2012Fusarium solani in different culture media and steady bright.]]>64752Poletto, T., M.F.B. Muniz, V.S. Fantinel, R.F. Favaretto, I. Poletto, L.R.S. Reiniger and E. Blume,2018Sirosporium diffusum.]]>10310318Ding, R., Y.L. Wang, B.X. Li and C.P. Xie,2010Pestalotiopsis mangiferae.]]>392024Ismail, A.M., G. Cirvilleri and G. Polizzi,2013Pestalotiopsis uvicola and Pestalotiopsis clavispora causing grey leaf spot of mango (Mangifera indica L.) in Italy.]]>135619625Steinrucken, T.V., A.K.H. Raghavendra, J.R. Powell, A. Bissett and R.D. van Klinken,201746157170de Assis Reges, J.T., M.N. de Jesus, S.D.R. da Silva, M.H. de Souza and J.W. Rodrigues,2019Pyricularia oryzae in the hosts of wheat, barley, rice and braquiaria.]]>281928Lima, J.S., J.E. Cardoso, R.C. Moreira, E.S. Alves and J.G.M. Melo,2012Lasiodiplodia theobromae and pathogenicity on acerola plants.]]>61016