Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2020.211.220Turnip mosaic virus in India Through Biological and Serological Evidences]]>KapoorShelly HandaAnil A. WalshJohn SharmaRajnish 42020194Background and Objective: Oilseed brassica are one of the most exploited agricultural commodities in International trade with diversified use in human and animal consumption besides their potential use in producing green energy in the form of biofuels. Turnip mosaic virus is one of the limiting factors for declining oil content in brassica. The present studies were therefore conducted to confirm the presence of this important virus in brassica through biological and serological assays. Materials and Methods: A total of 518 samples collected from 84 locations spanning across 5 states and 1 union territory from symptomatic plants were collected and assayed in DAS-ELISA using Turnip mosaic virus (TuMV) specific polyclonal antiserum. Biological and serological host range of the virus isolate was established and different varieties/breeding lines of oilseed brassica were screened for developing a resistance panel against TuMV. Results:Turnip mosaic virus incidence ranged between 0.6-8.3% in oilseed brassica and 0.2-17.6% in crucifer vegetables. Turnip mosaic viruswas recorded in very high concentration from radish as indicated by the optical density values. Mustard variety Tender Green was established as the best propagative host of Indian radish isolate of Turnip mosaic virus. Out of 32 varieties/breeding lines of oilseed brassica collected from different sources in India, 25 varieties/lines were found to be susceptible to Turnip mosaic virus under glasshouse conditions and DAS-ELISA further confirmed these findings. Conclusion: A radish isolate of Turnip mosaic virus has been identified on the basis of biological and serological assays and results obtained for screening of brassica germplasm against Turnip mosaic virus are expected to help in ascertaining the sources of resistance against this virus.]]>Jat, R.S., V.V. Singh, P. Sharma and P.K. Rai,20192019Kayum, M.A., H. Kim, U.J. Nath, J. Park, K.H., Kho, Y. Cho and I. Nou,2016Brassica rapa and B. oleracea: A review.]]>4135144Spencer, N.J., N.A. Phiri, S.L. Hughes, A. Mwaniki and S. Simons,2007Turnip mosaic virus, Cauliflower mosaic virus and Beet mosaic virus in three kenyan vegetables.]]>56317323Hughes, S.L., S.K. Green, D.J. Lydiate and J.A. Walsh,2002Turnip mosaic virus in Brassica rapa and B. napus and the analysis of genetic inheritance in selected lines.]]>51567573Ohshima, K., Y. Yamaguchi, R. Hirota, T. Hamamoto and K. Tomimura et al.,2002Turnip mosaic virus: Evidence of host adaptation, genetic recombination and geographical spread.]]>8315111521Nguyen, H.D., Y. Tomitaka, S.Y.W. Ho, S. Duchene and H.J. Vetten et al.,2013Turnip mosaic potyvirus probably first spread to Eurasian Brassica crops from wild orchids about 1000 years ago.]]>2013Tomlinson, J.A.,1987110661681Zherdev, A.V., S.V. Vinogradova, N.A. Byzova, E.V. Porotikova, A.M. Kamionskaya and B.B. Dzantier,20182018Haq, Q.M.R., K.M. Srivastava, R.K. Raizada, B.P. Singh, R.K. Jain, A. Mishra and D.D. Shukla,1994Turnip mosaic virus disease of Brassica compestris and B. juncea in India.]]>1405564Sevik, M.A.,20192019Astier, S., J. Albouy, Y. Maury, C. Robaglia and H. Lecoq,200857989989Mckinney, H.H.,1923Helminthosporium sativum.]]>26195217Dijkstra, J. and C.P. de Jager,19981998pp: 373-375pp: 373-375Walsh, J.A. and J.A. Tomlinson,1985Brassica napus ssp. oleifera).]]>107485495Nyalugwe, E.P., R.A.C. Jones, M.J. Barbetti and M.A. Kehoe,2015Turnip mosaic virus isolates.]]>6412151223Singh, R., A. Banerjee, S.K. Sharma, R. Bhagawati, Baruahs and S.V. Ngachan,2015Turnip mosaic virus occurrence in cole crops (Brassica spp.) from Arunachal Pradesh, India.]]>26211213Park, S., J. Park, T. Park and S. Hong,2020Turnip mosaic virus infecting Perilla frutescens in Korea.]]>2020Raybould, A.F., L.C. Maskul, M.L. Edwards, J.I. Cooper and A.J. Gray,1999Brassica oleracea.]]>141265275Farzadfar, S. and R. Pourrahim,2014Turnip mosaic virus from the Asian-BR population in Iran.]]>162824828Pallett, D.W., M.I. Thurston, M.C. Borja, M.L. Edwards and M. Alexander et al.,2002Brassica rapa ssp. sylvestris in southern England.]]>141163170Al-Saleh, M.A., I.M. Al-Shahwan, O.A. Abdalla and M.A. Amer,2009Eruca sativa in Saudi Arabia.]]>32738Sevik, M.A.,2016Brassica crops in the black sea region of Turkey.]]>66553557Parmar, N., A.K. Thakur, P. Kumar, P.D. Thakur and S.V. Bhardwaj,2017Turnip mosaic potyvirus (TuMV)-infecting radish (Raphanus sativus L.) crop in India.]]>2017Shevchenko, O., R. Yasaka, O. Tymchyshyn, T. Shevchenko and K. Ohshima,2019Turnip mosaic virus in Turkey.]]>156559569Korkmaz, S., Y. Tomitaka, S. Onder and K. Ohshima,2008Turnip mosaic virus.]]>5711551162Green, S.K.,1985Turnip Mosaic Virus strains in cruciferous hosts in Taiwan.]]>692831Tomimura, K., A.J. Gibbs, C.E. Jenner, J.A. Walsh and K. Ohshima,2003Turnip mosaic virus; comparison of 38 genomic sequences reveal a Eurasian origin and a recent emergence in east Asia.]]>1220992111Sardaru, P., L. Sinausia, S. Lopez-Gonalez, J. Zindovic, F. Sanchez and F. Ponz,2018Turnip mosaic virus is largely determined by the C-terminal region of P3 viral Protein.]]>1919841994Walsh, J.A. and C.E. Jenner,2002Turnip mosaic virus and the quest for durable resistance.]]>3289300Li, G., H. Lu, S. Zhang, S. Zhang and F. Li et al.,20196810351044