Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2020.200.210Meike JayantiRuth JokoTri 32020193Background and Objective: Bacterial endophytes, bacteria that reside within plant tissues without causing disease, can act as plant beneficial microbes. This study was carried out to determine the melon endophytic bacteria for their potential in plant growth enhancement and disease management strategies. Materials and Methods: The bacterial endophytes were explored from healthy melon plants in Yogyakarta and Central Java, Indonesia. The isolated bacteria were screened for their ability to produce extracellular enzymes (proteases and endoglucanase) and Indole-3-Acetic Acid phytohormone (IAA), to solubilize inorganic phosphate and to perform antagonistic effect against Acidovorax citrulli and Fusarium oxysporum f.sp. melonis. Bacteria that have the potential to be plant growth promoters and antagonists were molecularly identified by partial 16S rDNA sequence analysis. Results: Seven endophytic bacterial isolates were obtained, which were isolated from the roots, stems, petioles and leaves of healthy melon plant tissues. All the bacterial isolates were able to produce extracellular protease enzymes, to synthesis IAA and to inhibit the growth of F. oxysporum f.sp. melonis. Meanwhile, some of them could produce endoglucanase enzymes, solubilize inorganic phosphate and inhibit the growth of A. citrulli. Two representative isolates that showed the best results (KP B51 and Klt D01) were identified as genera Bacillus and Burkholderia,respectively. Conclusion: Molecular identification based on 16S rRNA gene sequences analysis suggested that the potential endophytic bacteria were close related to genera Bacillus and Burkholderia. The explored beneficial bacteria have possibility as alternatives for sustainable eco-friendly plant disease management in melon.]]>Schaad, N.W., E. Postnikova, A. Sechler, L.E. Claflin and A.K. Vidaver et al.,2008Acidovorax avenae as A. avenae (Manns 1905) emend., A. cattleyae (Pavarino, 1911) comb. nov., A. citrulli Schaad et al., 1978) comb. nov., and proposal of A. oryzae sp. nov.]]>31434446Yan, L., B. Hu, G. Chen, M. Zhao and R.R. Walcott,2017Acidovorax citrulli groups based on a detached melon fruit pathogenicity assay.]]>10713051311Risser, G., Z. Banihashemi and D.W. Davis,1976Fusarium oxysporum f. sp. melonis races and resistance genes in Cucumis melo]]>6611051106Chikh-Rouhou, H., R. Sta-Baba, C. Ayed, S. Belgacem, N. Boughalleb and M. Cherif,2013Fusarium oxysporum f.sp. Melonis in Tunisia]]>41593596Feng, J.J., J.Q. Li, R.R. Walcott, G.M. Zhang and L.X. Luo et al.,2013Acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbits]]>41115Windari, U., T. Joko and S. Subandiyah,20151915Burdman, S. and R. Walcott,2012Acidovorax citrulli: generating basic and applied knowledge to tackle a global threat to the cucurbit industry]]>13805815Horuz, S., R. Cetinkaya-Yildiz, M. Mirik and Y. Aysan,2014Acidovorax citrulli from melon in Turkey.]]>50179183Zuniga, T.L., T.A. Zitter, T.R. Gordon, D.T. Schroeder and D. Okamoto,1997Fusarium oxysporum f.sp. melonis causing Fusarium wilt of melon in New York.]]>81592596Ntui, V.O, G. Thirukkumaran, P. Azadi, R.S. Khan, I. Nakamura and M. Mii,2010Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot.]]>29943954Jean-Yves, P., K.B. Douglas, B.D. Martin, M.H. Robert, K.K. Harjeet and L.D. James,2011Fusarium wilt in transgenic ‘Lady Finger’ bananas.]]>911411148Sebastiani, M.S., P. Bagnaresi, S. Sestili, C. Biselli and A. Zechini et al.,2017Fusarium oxysporum f. sp. melonis race 1.2 pathosystem in susceptible and resistant plants.]]>2017Anitha, A. and M. Rabeeth,2009Fusarium wilt of tomato by bioformulation of Streptomyces griseus in green house condition.]]>1914Junaid, J.M., N.A. Dar, T.A. Bhat, A.H. Bhat and M.A. Bhat,201313957Sturz, A.V., B.R. Christie and J. Nowak,200019130Siddiqui, Z.A.,20052005111-142111-142Potshangbam, M., D. Sahoo, P. Verma, S. Verma, M.C.Kalita and S.I. Devi,2018Bacillus altitudinis Lc5, a biocontrol and plant growth-promoting endophyte strain isolated from indigenous black rice of manipur.]]>6118Ansary, W.R., F.R.K. Prince, E. Haque, F. Sultana and H.M. West et al.,2018Bacillus spp. from medicinal plants inhibit mycelial growth of Sclerotinia sclerotiorum and promote plant growth. ]]>73247256Mardanova, A.M., G.F. Hadieva, M.T. Lutfullin, I.V.E. Khilyas and L.F. Minnullina et al.,2016Bacillus subtilis strains with antifungal activity against the phytopathogenic fungi.]]>8120de los Santos Villalobos, S., R.I. Robles, F.I.P. Cota, J. Larsen, P. Lozano and J.M. Tiedje,2019Bacillus cabrialesii sp. nov., an endophytic plant growth promoting bacterium isolated from wheat (Triticum turgidum subsp. durum) in the Yaqui Valley, Mexico.]]>6939393946Sivasakthi, S., G. Usharani and P. Saranraj,2014Pseudomonas fluorescens and Bacillus subtilis: A review.]]>912651277Weilharter, A., B. Mitter, M.V. Shin, P.S.G. Chain, J. Nowak and A. Sessitsch,2011Burkholderia phytofirmans strain PsJN.]]>19333833384Taghavi, S., C. Garafola, S. Monchy, L. Newman and A. Hoffman et al.,200975748757Ismiyatuningsih, I., T. Joko and S. Hartono,2016Pectobacterium atrosepticum in major potato-growing areas in central java province, Indonesia.]]>116Verma, V.C., S.K. Gond, A. Mishra, A. Kumar, R.N. Kharwar and A.C. Gange,2009Azadirachta indica A. Juss.: Isolation, diversity and anti-microbial activity.]]>57749756Nawangsih, A.A., I. Damayanti, S. Wiyono and J.G. Kartika,2011186670Masanto, A. Wibowo, T. Joko, S. Subandiyah and K. Kageyama,2020Phytophthora palmivora causing black pod rot disease on Cacao (Theobroma cacao L.) in Indonesia.]]>192241Joko, T., D.N. Anggraeni, M. Irianti, B.S. Daryono, J. Widada and S. Subandiyah,201820183740Wardhika, C.M., Suryanti and T. Joko,2014Fusarium solani and Meloidogyne incognita on pepper. ]]>188994Joko, T., S. Subandiyah and S. Somowiyarjo,2000Xanthomonas campestris pv. citri.]]>63238Ash, K., Sushma and P.W. Ramteke,201811187194Sharma, V., R. Salwan and V. Shanmugam,2018Trichoderma saturnisporum isolate GITX-Panog (C) induced under mycoparasitic conditions.]]>1497380Joko, T., M. Umehara, T. Murata, H. Etoh, K. Izumori and S. Tsuyumu,2018Dickeya chrysanthemi EC16 by plant-derived sugars.]]>13141150Kowalczyk, M. and G. Sandberg,2001Arabidopsis.]]>12718451853Patil, V.,2011Azotobacter sp.]]>31416Dwimartina, F., T. Arwiyanto and T. Joko,2017Ralstonia syzygii subsp. syzygii.]]>11191198Handiyanti, M., T. Joko and S. Subandiyah,2018Burkholderia glumae, penyebab penyakit hawar malai padi.]]>2298107Joko, T., A. Soffan and M.S. Rohman,2019Pectobacterium carotovorum subsp. brasiliense.]]>2030423048Joko, T., A. Subandi, N. Kusumandari, A. Wibowo and A. Priyatmojo,20144712391250Nurjanah, N., T. Joko and S. Subandiyah,2017Pantoea ananatis isolated from garlic and shallot.]]>21120126Trianom, B., T. Arwiyanto and T. Joko,2018Ralstonia syzygii subsp. syzygii [In Indonesian].]]>22124131Kumar, S., G. Stecher and K. Tamura,20163318701874Trianom, B., T. Arwiyanto and T. Joko,201930107118Sibarani, S.M.A., T. Joko and S. Subandiyah,201923148155Vasconcelos, N.G., J. Croda and S. Simionatto,2018120198203Sessitsch, A., P. Hardoim, J. Döring, A. Weilharter and A. Krause et al.,2011252836Sharma, K.,201131112Xie, S.S, H.J. Wu, H.Y. Zang, L.M. Wu, Q.Q. Zhu and X.W. Gao,2014Bacillus subtilis OKB105.]]>27655663Adelskov, J. and B.K.C. Patel,2017Bacillus subtilis using genome sequences and its application to Bacillus subtilis subspecies stecoris strain D7XPN1, an isolate from a commercial food-waste degrading bioreactor]]>7116Santoyo, G., G. Moreno-Hagelsieb, M.D.C. Orozco-Mosqueda and B.R. Glick,20151839299Poretsky, R., R.L.M. Rodriguez, C. Luo, D. Tsementzi and K.T. Konstantinidis,20142014Chun, J. and K.S. Bae,2000Bacillus subtilis and related taxa based on partial gyrA gene sequences.]]>78123127Dalmastri, C., L. Pirone, S. Tabacchioni, A. Bevivino and L. Chiarini,2005recA PCR tests in the identification of Burkholderia cepacia complex environmental isolates.]]>2463945Mahenthiralingam, E., J. Bischof, S.K. Byrne, C. Radomski, J.E. Davies, Y. Av-Gay and P. Vandamme,2000Burkholderia cepacia complex, Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacian genomovars I and III. ]]>3831653173