Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2013.78.84Fusarium oxysporum Isolates from Three Cucurbit Species]]>ZhangShuo ZhaoBaixia LiuXian LiJibo GaoZenggui HuangXinyang 22013122Fusarium oxysporum is a main agriculture pathogen which
is responsible for wilting in cucumber, muskmelon and watermelon. In order to
study the genetic relationship and phylogeny of Fusarium oxysporum, 36
isolates were obtained from Liaoning province. The genetic relationship of Fusarium
was determined by using EF-1α and β-tubulin DNA sequencing as well
as a universally primed polymerase chain reaction (UP-PCR) analyses. The results
show that all 36 isolates were identified as being infected with F. oxysporum
by using the specific primers FOF1 and FOR1. Based on the combined sequence
and UP-PCR data, the 36 isolates and GenBank isolates were classified into three
major groups (Lineage1-3). These groups corresponded with the three clades of
F. oxysporum. Inspection of the sequence data revealed Lineage 1 and
Lineage 2 had similar sequences whereas Lineage 3 was significantly different
from the other two. And this finding matched the results of UP-PCR analysis.
However, the GenBank isolates were classified into Lineage 1 and none of the
three techniques applied in this study provided host-related resolution for
the pathotype isolates or the geographic origin of isolates. This study confirmed
that phylogenetic groups have little connection with virulent groups.]]>Baayen, R.P., K. O'Donnell, P.J.M. Bonants, E. Cigelnik, L.P. Kroon, E.J. Roebroeck and C. Waalwijk,2000Fusarium oxysporum complex identify monophyletic and non-monophyletic formae speciales causing wilt and rot diseases.]]>90891900Baixia, Z., G. Zenggui and Z. Jinghua,2009Fusarium Species from greenhouse soils of melon crops with UP-PCR.]]>104650Zhao, B.X., Z.G. Gao, J.H. Zhuang, X.F. Zhang and H. Zhao,2009Fusarium population isolated from greenhouse melon soils.]]>20857862Benthy, S., K.G. Pegg, N.Y. Moore, R.D. Davis and I.W. Buddenhagen, 1998Fusarium oxysporum f. sp. cubense analyzed by DNA fingerprinting.]]>8812831293Bulat,S.A., M. Lubeck, N. Mironenko, D.F. Jensen and P.S. Lubeckb,1998Trichoderma and Gliocladium.]]>102933943Elad, Y., I. Chet, P. Boyle and Y. Henis,1983Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii-Scanning electron microscopy and fluorescence microscopy.]]>738588Namiki, F., T. Shiomi, T. Kayamura and T. Tsuge, 1994Fusarium oxysporum causing wilts of cucurbits by dna fingerprinting with nuclear repetitive dna sequences. ]]>6026842691Hammerschmidt, R., J.P. Metraux and L.C. van Loon,200110716Brown, J.K. and C.G. Simpson,1994Erysiphe graminis f. sp. hordei.]]>26172178Abd-Elsalam, K., M. Khalil, A.H. Aly and A.M. Asran-Amal,2002Fusarium oxysporum f.sp. Vasinfectum isolates revealed by UP-PCR and AFLP markers.]]>41252258Kim, D.H., R.D. Martyn and C.W. Magil,1993Fusarium oxysporum in the cucurbitaceae.]]>839197Leslie, J.F., K.A. Zeller and B.A. Summerell,200159107117Liu, D., S. Coloe, R. Baird and J. Pedersen,200038471471Lubeck, M. and H. Poulsen,2001Rhizoctonia solani complex.]]>2018389Marlatt, M.L., J.C. Correll, P. Kaufmann and P.E. Cooper,1996Fusarium oxysporum f. sp. lycopersici race 3 in the United States.]]>8013361342McMillan, R.T.,1986Fusarium oxysporum from either cucumber or watermelon pathogenic to both crop species.]]>109101105Bogale, M., B.D. Wingfield, M.J. Wingfield and E.T. Steenkamp,2006Fusarium oxysporum isolates from Ethiopia using AFLP, SSR and DNA sequence analyses.]]>235166Namiki, F., M. Matsunaga, M. Okuda, I. Inoue, K. Nishi, Y. Fujita and T. Tsuge,2001Fusairum oxsporum f.sp. Melonis.]]>14580584O'Donnell, K., H.C. Kistler, E. Cigelnik and R.C. Ploetz,1998Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies.]]>9520442049O'Donnell, K., H.C. Kistler, B.K. Tacke and H.H. Casper,2000Fusarium graminearum, the fungus causing wheat scab.]]>9779057910Zhang, S., Z.G. Gao, J.H. Zhuang, B.X. Zhao, X.F. Zhang, N.A. Ming-Hui,2010Fusarium oxysporum isolates from watermelon melon and cucumber in liaoning Province with UP-PCR.]]>11923Yangxiu, J. and G. Guanlin,2002284345Yli-Mattila, T., R.L. Mach, A. Alekhina, S.A. Bulat and S. Koskinen et al.,2004Fusarium langsethiae to Fusarium poae and Fusarium sporotrichioides as inferred by IGS, ITS, β-tubulin sequences and UP-PCR hybridization analysis.]]>95267285Lee, H.J., H.J. Cho, K.A., Lee, M.S. Lee and Y.S. Shin et al.,20011098105