Plant Pathology Journal1812-53871812-5425Asian Network for Scientific Information10.3923/ppj.2007.44.50FarragEman S.ZiedanEl-Sayed H.MahmoudSabry Y.1200761Systemic Acquired Resistance (SAR) in cucumber plants against powdery mildew disease, caused by Sphaerotheca fuliginea (Schlechtend Fr.) Pollacci, was induced by localized infection in cucumber cotyledons with Tobacco Necrosis Virus (TNV). Inoculation of the two cotyledon leaves significantly reduced powdery mildew severity on cucumber true leaves similar to the effect of fungicide. TNV-local lesions developed plants still protected against powdery mildew for 16 days. The level of protection was higher in the upper true leaves when compared with the bottom ones. Antifungal protein was extracted and partially purified by DEAE-cellulose column chromatography from cucumber TNV-inoculated plants only. Bioassay detection for antifungal activity indicated that, 0.6 M NaCl fraction had the highest activity. SDS-PAGE of partially purified 0.6 M NaCl fraction protein indicated the presence of a single protein band with a molecular weight of about 30 kDa. This protein was extracted from upper TNV-uninoculated true leaves of plants inoculated on the cotyledon leaves, which acquired systemic resistance against powdery mildew challenge inoculation. In vitro study of the antifungal activity of these proteins showed that only 0.6 M NaCl fraction has direct antifungal activity towards S. fuliginea conidial spores. The induced systemic resistance was not accompanied with the activity of β-1, 3 glucanase.]]>Abeles, F.B. and L.E. Forrence,1970Phaseolus vulgaris L.]]>45395400Anfoka, G. and H. Buchenauer,1997Phytophthora infestans by pre-inoculation with tobacco necrosis virus.]]>5085101Bradford, M.M.,197672248254Cheng, J. and J. Kuc,1995Colletotrichum lagenarium and tobacco necrosis virus.]]>8899905Dean, R.A. and J. Kuc,198676966970Dean, R. and J. Kuc,19871987pp: 410-484pp: 410-484Ehara, Y., T. Kato and S. Yamanka,1982331421Enkerli, J., U. Gisi and E. Mosinger,1993Phtophthora infestans in tomato and the role of pathogenesis related proteins.]]>356783Faccioli, G. and R. Capponi,1983Chenopodium amaranticolor locally infected by tobacco necrosis virus: 1. Extraction, partial purification biological and chemical properties.]]>106289301Fegla, G.I., H.M. Sheir and S.A.E. Kazzaz,19853014611474Gorlach, J., S. Volrath, G. Knaufbeiter, G. Hengy and U. Beckhou,19968629643Hammerschmidt, R. and J. Kuc,1982206171Ivanovic, D., M. Radosavlyevic, J. Levic and M. Ivanovic,1994Fusarium graminearum Development in Maize Plants Infected by Maize Dwarf Mosaic Virus Ninth Congress of the Mediterranean Phytopathological.]]>pp: 97-99pp: 97-99Jeun, Y.C. and H. Buchenauer,2001Phytophthora infestans after pre-treatment with 3-aminobutyric acid or tobacco necrosis virus.]]>149141153Kessmann, H., T. Staub, C. Hofmann, T. Maetzke and J. Herzog et al.,199432439459Kessmann, H., M. Oostendrop, T. Staup, J. Goerlach, L. Friedrich, K. Lawton and J. Ryals,19961996pp: 961966Kimati, H., C.O.M. Cardoso and A. Bergamin Filho,19801980pp: 251-269pp: 251-269Kimati, H., N.G. Fernandes, J. Soave, C. Kurozawa, F.B. Neto and W. Bettiol,19972nd Edn.,pp: 213pp: 213Kogel, K.H., U. Beckhove, J. Dreschers, S. Munch and Y. Romme,199410612691277Kuc, J.,19901990pp: 355-373pp: 355-373Laemmli, U.K.,1970227680685Grath, M.T.M.,1996Sphaerotheca fuliginea populations following fungicide usage over one season.]]>80633639Grath, M.T.M., H. Staniszewska and N. Shishkoff,1996Sphaerotheca fuliginea populations in the united states.]]>80697703Grath, M.T.M., and N. Shiskoff,199686S53S53Mosa, A.A.,199742241255Pan, S.Q., S.X. Ye and J. Kuc,1989182136140Pan, S.Q., S.X. Ye and J. Kuc,1993Prenospora tabacina or leaf inoculation with tobacco mosaic virus.]]>83906909Russel, G.H.,1965Alternaria species on leaves of sugar beet infected with yellowing viruses 1Some effects of four fungicides on two beet varieties.]]>56111118Ryals, J., S. Uknes and E. Ward,199410411091112Schmele, I. and H. Kauss,199037221228Sela, I., I. Harpaz and Y. Birk,1966287178Siegrist, J., W. Jeblick and H. Kauss,1994Cucumis sativus L.) segments exhibiting acquired resistance.]]>10513651374Siegrist, J., D. Glenewinkel, C. Kolle and M. Schmitke,1997104599610Sitterly, W.R.,19781978pp: 259-379pp: 259-379Snedecor, G.W. and W.G. Cochran, 19806th Edn.,Sticher, L., B.M. Mani and J.P. Metraux,199735235270Loon, L.C.V.,1997103753765Ye, X.S., U. Jarlfors, S. Tuzum, S.Q. Pan and J. Kuc,1992Prenospora tabacina) induced by tobacco mosaic virus.]]>704957