Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2019.671.676Lawsonia inermis Linn. Extracts on Blood Glucose Level in Normal and Streptozotocin-Induced Diabetic Rats]]>WidyawatiT. PaneY.S. YusoffN.A. 72019187Background and Objective:Lawsonia inermis Linn. is one of the common medicinal plants that are being used widely to treat diabetes in Medan, North Sumatera, Indonesia. Scientific evidence to confirm its efficacy is still lacking. Hence, this study was designed to evaluate the effect of L. inermis leaf extracts on glucose level in normal and streptozotocin-induced diabetic rats. Materials and Methods: Serial extraction of Lawsonia inermis Linn. leaf was conducted to obtain the respective n-hexane (HE), ethyl acetate (EAE), ethanol (EE) and distilled water (A1E) extracts. Another water extract (A2E) was obtained using infusion technique. These extracts were administered orally at the dose of 1 g kg1 b.w. to normal and streptozotocin-induced diabetic rats. An acute hypoglycemic, glucose tolerance and antihyperglycemic effects were assessed. Qualitative phytochemical screenings were conducted prior to the in vivo tests. Results: The phytochemical screenings revealed the presence of steroid and triterpenoid in HE, alkaloid, tannin, flavonoids, saponin and glycoside in EE and EAE. While in A1E and A2E, tannin, flavonoid, saponin and glycoside were identified. Hypoglycemic test in normal rats showed HE was able to decrease blood glucose level (BGL) significantly (p<0.05). A1E and A2E were able to inhibit the rise of BGL after being challenged with glucose intraperitoneally. Furthermore, administration of single dose of extracts showed that only EAE exerted significant antihyperglycemic activity. Conclusion: All the extracts of Lawsonia inermis Linn. leaf, except for EE, have shown certain degree of antidiabetic activity with possible different mechanisms of antidiabetic action.]]>Cragg, G.M. and D.J. Newman,2013183036703695Kumar, M., P. Kaur, M. Chandel, A.P. Singh, A. Jain and S.J. Kaur,2017Lawsonia inermis L. leaves against 2-acetylaminofluorene induced hepatic damage in male Wistar rats.]]>2017Kamal, M. and T. Jawaid,2010Lawsonia inermis Linn.: A review.]]>16268Salih, A.M., F.H. Kakamad, R.Q. Salih, D.A. Hussein and H.A. Hassan et al.,2017Lawsonia inermis (Henna) on wound healing in Sprague-Dawley rats: A pilot study.]]>184142Widyawati, T., W.W. Purnawan, I.J. Atangwho, N.A. Yusoff, M. Ahmad and M.Z. Asmawi,2015Syzygium polyanthum (wight) leaf extract, the most commonly used herb among diabetic patients in Medan, North Sumatera, Indonesia.]]>616981704Goel, R., D. Bhatia, S.J. Gilani and D. Katiyar,20121100107Syamsudin, Inawati and H. Winarno,2008Lawsonia inermis Linn) leaves extract on blood sugar level: An experimental study.]]>22023Chikaraddy, A., Y. Maniyar and B. Mannapur,2012Lawsonia inermis Linn. (henna) in alloxan induced diabetic albino rats.]]>2287292Singh, S., N. Verma, R. Karwasra, P. Kalra, R. Kumar and Y.K. Gupta,2015Lawsonia inermis leaves on lipid profile in alloxan-induced diabetic rats.]]>36107112Imam, H., N.U. Mahbub, M.F. Khan, H.K. Hana and M.M.R. Sarker,2013Lawsonia inermis.]]>1617961800Pandey, A. and S. Tripathi,20142115119Handa, S.S., S.P.S. Khanuja, G. Longo and D.D. Rakesh,2008Pages: 260Pages: 260Evans, W.C.,200916th Edn.,Pages: 616Pages: 616Harborne, J.B.,19872nd Edn.,Pages: 354Pages: 354Farnsworth, R.N.,196655225276Chaudhary, G., S. Goyal and P. Poonia,2010Lawsonia inermis Linnaeus: A phytopharmacological review.]]>2119198Sasidharan, S., Y. Chen, D. Saravaran, K.M. Sundram and L.Y. Latha,20118110Raja, W., M. Ovais and A. Dubey,2013Lawsonia inermis leaf extract.]]>43336Tiong, S.H., C.Y. Looi, H. Hazni, A. Arya and M. Paydar et al.,2013Catharanthus roseus (L.) G. Don.]]>1897709784Daisy, P., R. Jasmine, S. Ignacimuthu and E. Murugan,2009Elephantopus scaber L. an ethnomedicinal plant with antidiabetic activity.]]>16252257Weinberg, S.L. and S.K. Abramowitz,20082nd Edn.,Pages: 780Pages: 780Babu, P.V.A., D. Liu and E.R. Gilbert,20132417771789Vinayagam, R. and B. Xu,20152015Benalla, W., S. Bellahcen and M. Bnouham,20106247254Uma, D.B., C.W. Ho and W.M.W. Aida,2010Lawsonia inermis) leaves.]]>39119128Widyawati, T., N.A.B. Roslan, N.A. Yusoff, K.N.B. Abdul Razak, Y.M. Fei, M.Z. Asmawi and M. Ahmad,2016Eugenia polyantha leaves extracts.]]>9465471Kumar, S., S. Narwal, V. Kumar and O. Prakash,201151929Goncalves, R., N. Mateus and V. de Freitas,2011125665672Bodmer, M., C. Meier, S. Krahenbuhl, S.S. Jick and C.R. Meier,20083120862091Tripathi, A.K. and S. Kohli,2014Syzygium cumini (Linn.) barks (Myrtaceae) on streptozotocin-induced diabetic rats.]]>117181Inzucchi, S.E., M.R. Bergenstal, J.B. Buse, M. Diamant and E. Ferrannini et al.,201538140149Brunton, L.L., John S. Lazo and L.L. Parker,200611th Edn.,Pages: 1984Pages: 1984