Pakistan Journal of Nutrition1680-51941994-7984Asian Network for Scientific Information10.3923/pjn.2019.1014.1020El-BashitiTarek A. MasadAtef A. MoslehFarida N. MadiYasmeen M. Abu 1120191811Background and Objective: Biofilm is a complex microbial community highly resistant to antimicrobials agents. The formation of biofilms in biotic and abiotic surfaces is associated with high rates of morbidity and mortality and considered as a major factor of bacterial pathogenicity. In the present study the antimicrobial and antibiofilm activities of 5 plant extracts (Glycyrrhiza glabra roots, Laurus nobili, Malus domestica peels, Melissa officinalis and Lagenaria siceraria peels) were evaluated against clinical isolated microorganisms (E. coli, S. aureus, P. aeruginosa and K. pneumonia). Materials and Methods: The extracts of medicinal plants were prepared using microwave assisted method by ethanol and water. Results: The results of this study revealed that, the aquatic extract of M. domestica peels showed powerful antimicrobial activity against E. coli with MIC value 1.56 mg mL1, the ethanolic extract of G. glabra roots showed good antimicrobial activity against P. aeruginosa with minimum inhibitory concentration (MIC) of 12.5 mg mL1. Most plant extracts in this study gave the minimum bactericidal concentration (MBC) ≥200 mg mL1 against tested bacteria. However, the aquatic extract of L. nobili at concentration of 12.5 mg mL1 showed the highest antibiofilm activity (86.7%) against S. aureus biofilm formation. The ethanolic extract of M. domestica peels at concentration of 25 mg mL1 showed the highest inhibition rate (90%) against P. aeruginosa biofilm formation. Conclusion: These results revealed the importance of the tested extracts in the control of common human pathogenic micro-organisms. Plant extracts used in this study may contain potential antimicrobial and antibiofilm components that may be of great use for the development of new therapies against the most common infectious bacterial isolates.]]>Abiramasundari, P., V. Priya, G.P. Jeyanthi and S.G. Devi,2011Cocculus hirsutus.]]>32631Davies, J. and D. Davies,201074417433Ruban, P. and K. Gajalaksmi,20168208211Vasudevan, R.,201418498Parsek, M. and P.K. Singh,200357677701Rasmussen, T.B. and M. Givskov,2006296149161Sun, F., F. Qu, Y. Ling, P. Mao, P. Xia, H. Chen and D. Zhou,20138877886Musk, D.J., D.A. Banko and P.J. Hergenrother,2005Pseudomonas aeruginosa.]]>12789796Bobbarala, V., P.K. Katikala, K.C. Naidu and S. Penumajji,2009Aspergillus niger F2723.]]>208790Elbashiti, T.A., A.A. Elmanama and A.A. Masad,2011Escherichia coli and Staphylococcus aureus.]]>55762Jouda, M.M., T. Elbashiti, A. Masad and M. Albayoumi,201652333Elbashiti, T.,2016Punica granatum, Actinidia deliciosa and Citrus maxima fruit extracts against some human pathogens.]]>5169180Jameela, M., A. Mohideen, K. Sunitha and M. Narayanan,201125760Bauer, A.W., W.M.M. Kirby, J.C. Sherris and M. Turck,196645493496Shohayeb, M. and E. Halawani,2012N. sativa L.]]>20182189Mostafa, A.A., A.A. Al-Askar, K.S. Almaary, T.M. Dawoud, E.N. Sholkamy and M.M. Bakri,201825361366Pour, N.K., D.H. Dusane, P.K. Dhakephalkar, F.R. Zamin, S.S. Zinjarde and B.A. Chopade,2011Acinetobacter baumannii strains isolated from urinary tract infection and urinary catheters.]]>62328338Elmanama, A.A., R.I. Abu-Dan, R.N. Eqtifan, A.A. Shomar and M.R. Rifi,2019Pseudomonas aeruginosa isolated from Al-Shifa hospital and their susceptibility to acetic acid.]]>271925Musleh, R.M. and A.Q. Jebur,2014Klebsiella pneumoniae on catheter, glass and stainless-steel surfaces.]]>5512081212Bazargani, M.M. and J. Rohloff,2016Staphylococcus aureus and Escherichia coli biofilms.]]>61156164Nitalikar, M.M., K.C. Munde, B.V. Dhore and S.N. Shikalgar,2010Glycyrrhiza glabra root extract.]]>2899901Mahady, G.B., S.L. Pendland, A. Stoia, F.A. Hamill, D. Fabricant, B.M. Dietz and L.R. Chadwick,2005In vitro susceptibility of helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders.]]>19988991Al-Mariri, A. and M. Safi,2014In vitro antibacterial activity of several plant extracts and oils against some gram-negative bacteria.]]>393643Sibanda, T. and A.I. Okoh,2007628862896Sigei, E.C., M. Muturi and C. Bii,2015Camellia sinensis crude extract, mixture with milk, on selected pathogenic and mycotoxic fungi.]]>910701080Adwan, G. and M. Mhanna,2008Staphylococcus aureus strains isolated from clinical specimens.]]>3134139Namasivayam, S.K.R. and E.A. Roy,2013Escherichia coli.]]>5486489Chakotiya, A.S., A. Tanwar, A. Narula and R.K. Sharma,2016Pseudomonas aeruginosa: Effects of Glycyrrhiza glabra on membrane permeability and inhibition of efflux activity and biofilm formation in Pseudomonas aeruginosa and its in vitro time-kill activity.]]>9898105Waldrop, R., A. McLaren, F. Calara and R. McLemore,2014in vitro.]]>47233053310 Flemming, H.C., J. Wingender, T. Griegbe and C. Mayer,20002000pp: 19-34.pp: 19-34.